Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc01704 and SMc01703 are separated by 513 nucleotides SMc01703 and SMc01702 are separated by 22 nucleotides SMc01702 and SMc01701 are separated by 88 nucleotides SMc01701 and SMc02181 are separated by 249 nucleotides
SMc01704: SMc01704 - hypothetical protein, at 508,266 to 508,943
SMc01704
SMc01703: SMc01703 - hypothetical protein, at 509,457 to 509,888
SMc01703
SMc01702: SMc01702 - oxidoreductase, at 509,911 to 510,897
SMc01702
SMc01701: SMc01701 - hypothetical protein, at 510,986 to 511,570
SMc01701
SMc02181: SMc02181 - bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase, at 511,820 to 515,521
SMc02181
Position (kb) 509
510
511 Strain fitness (log2 ratio) -2
-1
0
1 at 509.003 kb on - strand at 509.003 kb on - strand at 509.046 kb on + strand at 509.047 kb on - strand at 509.047 kb on - strand at 509.047 kb on - strand at 509.098 kb on + strand at 509.173 kb on - strand at 509.200 kb on - strand at 509.200 kb on - strand at 509.200 kb on - strand at 509.345 kb on + strand at 509.345 kb on + strand at 509.345 kb on + strand at 509.345 kb on + strand at 509.345 kb on + strand at 509.345 kb on + strand at 509.345 kb on + strand at 509.346 kb on - strand at 509.346 kb on - strand at 509.346 kb on - strand at 509.346 kb on - strand at 509.351 kb on + strand at 509.352 kb on - strand at 509.352 kb on - strand at 509.352 kb on - strand at 509.354 kb on - strand at 509.354 kb on - strand at 509.509 kb on + strand, within SMc01703 at 509.510 kb on - strand, within SMc01703 at 509.609 kb on + strand, within SMc01703 at 509.610 kb on - strand, within SMc01703 at 509.693 kb on - strand, within SMc01703 at 509.726 kb on - strand, within SMc01703 at 509.729 kb on - strand, within SMc01703 at 509.834 kb on - strand, within SMc01703 at 509.897 kb on + strand at 510.340 kb on + strand, within SMc01702 at 510.340 kb on + strand, within SMc01702 at 510.371 kb on + strand, within SMc01702 at 510.372 kb on - strand, within SMc01702 at 510.449 kb on + strand, within SMc01702 at 510.512 kb on + strand, within SMc01702 at 510.512 kb on + strand, within SMc01702 at 510.512 kb on + strand, within SMc01702 at 510.513 kb on - strand, within SMc01702 at 510.601 kb on - strand, within SMc01702 at 510.612 kb on + strand, within SMc01702 at 510.614 kb on + strand, within SMc01702 at 510.614 kb on + strand, within SMc01702 at 510.615 kb on - strand, within SMc01702 at 510.904 kb on + strand at 510.904 kb on + strand at 510.905 kb on - strand at 510.905 kb on - strand at 510.950 kb on + strand at 510.962 kb on + strand at 510.963 kb on - strand at 511.401 kb on + strand, within SMc01701 at 511.402 kb on - strand, within SMc01701 at 511.613 kb on + strand at 511.767 kb on - strand at 511.767 kb on - strand at 511.767 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 509,003 - -0.1 509,003 - +0.2 509,046 + +0.1 509,047 - -0.2 509,047 - +0.9 509,047 - +0.3 509,098 + -0.1 509,173 - +0.3 509,200 - -0.9 509,200 - +0.1 509,200 - +0.1 509,345 + +0.7 509,345 + +1.4 509,345 + +0.3 509,345 + +1.1 509,345 + -1.0 509,345 + -0.1 509,345 + -0.2 509,346 - +0.7 509,346 - +0.4 509,346 - +1.3 509,346 - -0.7 509,351 + +0.3 509,352 - +0.4 509,352 - -0.1 509,352 - -0.8 509,354 - +0.2 509,354 - -2.1 509,509 + SMc01703 0.12 -0.8 509,510 - SMc01703 0.12 +0.1 509,609 + SMc01703 0.35 -0.1 509,610 - SMc01703 0.35 -0.3 509,693 - SMc01703 0.55 +0.4 509,726 - SMc01703 0.62 +1.6 509,729 - SMc01703 0.63 +0.5 509,834 - SMc01703 0.87 -0.2 509,897 + -0.1 510,340 + SMc01702 0.43 +1.1 510,340 + SMc01702 0.43 +1.5 510,371 + SMc01702 0.47 +0.1 510,372 - SMc01702 0.47 +0.6 510,449 + SMc01702 0.55 -0.0 510,512 + SMc01702 0.61 +0.4 510,512 + SMc01702 0.61 -0.6 510,512 + SMc01702 0.61 +0.5 510,513 - SMc01702 0.61 -0.9 510,601 - SMc01702 0.70 +0.2 510,612 + SMc01702 0.71 +0.6 510,614 + SMc01702 0.71 +0.9 510,614 + SMc01702 0.71 +0.1 510,615 - SMc01702 0.71 +0.4 510,904 + +0.7 510,904 + -0.3 510,905 - +0.2 510,905 - +0.1 510,950 + -0.6 510,962 + -0.6 510,963 - -0.0 511,401 + SMc01701 0.71 +1.3 511,402 - SMc01701 0.71 -0.4 511,613 + +0.2 511,767 - -0.1 511,767 - -0.8 511,767 - +0.2
Or see this region's nucleotide sequence