Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1347 and SMa1349 are separated by 25 nucleotides SMa1349 and SMa1351 are separated by 2 nucleotides SMa1351 and SMa1353 are separated by 9 nucleotides
SMa1347: SMa1347 - dehydrogenase, at 737,144 to 738,118
SMa1347
SMa1349: SMa1349 - GntR family transcriptional regulator, at 738,144 to 738,836
SMa1349
SMa1351: SMa1351 - enolase, at 738,839 to 740,047
SMa1351
SMa1353: SMa1353 - epimerase, at 740,057 to 740,944
SMa1353
Position (kb) 738
739
740
741 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 737.925 kb on - strand, within SMa1347 at 737.943 kb on - strand, within SMa1347 at 738.071 kb on - strand at 738.071 kb on - strand at 738.146 kb on - strand at 738.263 kb on - strand, within SMa1349 at 738.266 kb on + strand, within SMa1349 at 738.266 kb on + strand, within SMa1349 at 738.272 kb on + strand, within SMa1349 at 738.273 kb on - strand, within SMa1349 at 738.273 kb on - strand, within SMa1349 at 738.273 kb on - strand, within SMa1349 at 738.316 kb on + strand, within SMa1349 at 738.316 kb on + strand, within SMa1349 at 738.321 kb on - strand, within SMa1349 at 738.346 kb on + strand, within SMa1349 at 738.347 kb on - strand, within SMa1349 at 738.347 kb on - strand, within SMa1349 at 738.349 kb on + strand, within SMa1349 at 738.451 kb on + strand, within SMa1349 at 738.491 kb on - strand, within SMa1349 at 738.491 kb on - strand, within SMa1349 at 738.491 kb on - strand, within SMa1349 at 738.569 kb on - strand, within SMa1349 at 738.650 kb on - strand, within SMa1349 at 738.650 kb on - strand, within SMa1349 at 738.654 kb on - strand, within SMa1349 at 738.795 kb on + strand at 738.795 kb on + strand at 738.796 kb on - strand at 738.837 kb on - strand at 738.840 kb on + strand at 738.879 kb on - strand at 738.914 kb on + strand at 738.915 kb on - strand at 739.121 kb on - strand, within SMa1351 at 739.267 kb on + strand, within SMa1351 at 739.267 kb on + strand, within SMa1351 at 739.268 kb on - strand, within SMa1351 at 739.379 kb on - strand, within SMa1351 at 739.434 kb on + strand, within SMa1351 at 739.434 kb on + strand, within SMa1351 at 739.498 kb on - strand, within SMa1351 at 739.551 kb on + strand, within SMa1351 at 739.551 kb on + strand, within SMa1351 at 739.551 kb on + strand, within SMa1351 at 739.551 kb on + strand, within SMa1351 at 739.573 kb on + strand, within SMa1351 at 739.574 kb on - strand, within SMa1351 at 739.654 kb on + strand, within SMa1351 at 739.693 kb on + strand, within SMa1351 at 739.737 kb on + strand, within SMa1351 at 739.738 kb on - strand, within SMa1351 at 739.762 kb on + strand, within SMa1351 at 739.803 kb on + strand, within SMa1351 at 739.831 kb on - strand, within SMa1351 at 739.967 kb on - strand at 739.967 kb on - strand at 740.173 kb on + strand, within SMa1353 at 740.198 kb on - strand, within SMa1353 at 740.391 kb on + strand, within SMa1353 at 740.391 kb on + strand, within SMa1353 at 740.391 kb on + strand, within SMa1353 at 740.391 kb on + strand, within SMa1353 at 740.391 kb on + strand, within SMa1353 at 740.391 kb on + strand, within SMa1353 at 740.392 kb on - strand, within SMa1353 at 740.392 kb on - strand, within SMa1353 at 740.392 kb on - strand, within SMa1353 at 740.392 kb on - strand, within SMa1353 at 740.392 kb on - strand, within SMa1353 at 740.392 kb on - strand, within SMa1353 at 740.392 kb on - strand, within SMa1353 at 740.392 kb on - strand, within SMa1353 at 740.499 kb on + strand, within SMa1353 at 740.645 kb on + strand, within SMa1353 at 740.646 kb on - strand, within SMa1353 at 740.687 kb on + strand, within SMa1353 at 740.688 kb on - strand, within SMa1353 at 740.688 kb on - strand, within SMa1353 at 740.689 kb on + strand, within SMa1353 at 740.690 kb on - strand, within SMa1353 at 740.703 kb on + strand, within SMa1353 at 740.704 kb on - strand, within SMa1353 at 740.704 kb on - strand, within SMa1353 at 740.874 kb on + strand at 740.945 kb on - strand at 740.967 kb on - strand at 740.967 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 737,925 - SMa1347 0.80 -0.2 737,943 - SMa1347 0.82 -0.1 738,071 - +0.8 738,071 - -0.6 738,146 - -0.3 738,263 - SMa1349 0.17 +0.5 738,266 + SMa1349 0.18 -0.0 738,266 + SMa1349 0.18 +0.2 738,272 + SMa1349 0.18 +1.0 738,273 - SMa1349 0.19 +0.4 738,273 - SMa1349 0.19 -0.8 738,273 - SMa1349 0.19 -0.4 738,316 + SMa1349 0.25 +0.2 738,316 + SMa1349 0.25 +0.3 738,321 - SMa1349 0.26 -0.3 738,346 + SMa1349 0.29 -0.4 738,347 - SMa1349 0.29 +0.3 738,347 - SMa1349 0.29 +0.8 738,349 + SMa1349 0.30 +0.6 738,451 + SMa1349 0.44 -0.8 738,491 - SMa1349 0.50 -2.0 738,491 - SMa1349 0.50 -0.5 738,491 - SMa1349 0.50 +0.6 738,569 - SMa1349 0.61 -1.7 738,650 - SMa1349 0.73 -0.4 738,650 - SMa1349 0.73 +0.6 738,654 - SMa1349 0.74 -0.1 738,795 + +0.8 738,795 + -0.5 738,796 - +0.4 738,837 - +0.4 738,840 + +0.2 738,879 - +0.8 738,914 + -0.3 738,915 - -2.6 739,121 - SMa1351 0.23 -1.0 739,267 + SMa1351 0.35 -0.6 739,267 + SMa1351 0.35 +0.8 739,268 - SMa1351 0.35 +0.5 739,379 - SMa1351 0.45 +1.1 739,434 + SMa1351 0.49 +0.2 739,434 + SMa1351 0.49 +0.1 739,498 - SMa1351 0.55 +2.1 739,551 + SMa1351 0.59 +0.7 739,551 + SMa1351 0.59 -1.4 739,551 + SMa1351 0.59 +0.7 739,551 + SMa1351 0.59 +1.0 739,573 + SMa1351 0.61 +0.0 739,574 - SMa1351 0.61 +0.3 739,654 + SMa1351 0.67 -0.1 739,693 + SMa1351 0.71 -0.4 739,737 + SMa1351 0.74 -0.3 739,738 - SMa1351 0.74 +0.5 739,762 + SMa1351 0.76 -0.8 739,803 + SMa1351 0.80 -2.8 739,831 - SMa1351 0.82 +1.3 739,967 - -0.3 739,967 - -1.3 740,173 + SMa1353 0.13 -0.1 740,198 - SMa1353 0.16 +0.7 740,391 + SMa1353 0.38 +0.0 740,391 + SMa1353 0.38 -1.2 740,391 + SMa1353 0.38 +0.2 740,391 + SMa1353 0.38 -1.0 740,391 + SMa1353 0.38 -0.1 740,391 + SMa1353 0.38 -0.7 740,392 - SMa1353 0.38 -0.4 740,392 - SMa1353 0.38 +0.4 740,392 - SMa1353 0.38 +0.5 740,392 - SMa1353 0.38 +0.1 740,392 - SMa1353 0.38 -0.3 740,392 - SMa1353 0.38 +0.8 740,392 - SMa1353 0.38 +0.8 740,392 - SMa1353 0.38 -0.8 740,499 + SMa1353 0.50 +0.1 740,645 + SMa1353 0.66 +0.2 740,646 - SMa1353 0.66 +0.1 740,687 + SMa1353 0.71 +0.5 740,688 - SMa1353 0.71 -2.0 740,688 - SMa1353 0.71 -1.0 740,689 + SMa1353 0.71 +0.2 740,690 - SMa1353 0.71 +0.2 740,703 + SMa1353 0.73 -0.4 740,704 - SMa1353 0.73 +1.6 740,704 - SMa1353 0.73 +0.1 740,874 + -0.2 740,945 - +0.1 740,967 - -0.1 740,967 - -0.2
Or see this region's nucleotide sequence