Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21488 and SM_b21489 overlap by 4 nucleotides SM_b21489 and SM_b22024 overlap by 4 nucleotides SM_b22024 and SM_b21490 are separated by 135 nucleotides SM_b21490 and SM_b21491 are separated by 20 nucleotides
SM_b21488: SM_b21488 - cytochrome o ubiquinol oxidase subunit I, at 1,409,489 to 1,411,498
_b21488
SM_b21489: SM_b21489 - cytochrome O ubiquinol oxidase subunit III, at 1,411,495 to 1,412,127
_b21489
SM_b22024: SM_b22024 - cytochrome O ubiquinol oxidase CyoD, at 1,412,124 to 1,412,525
_b22024
SM_b21490: SM_b21490 - hypothetical protein, at 1,412,661 to 1,413,416
_b21490
SM_b21491: SM_b21491 - hypothetical protein, at 1,413,437 to 1,413,967
_b21491
Position (kb)
1412
1413 Strain fitness (log2 ratio)
-2
-1
0
1 at 1411.166 kb on + strand, within SM_b21488 at 1411.216 kb on + strand, within SM_b21488 at 1411.256 kb on - strand, within SM_b21488 at 1411.256 kb on - strand, within SM_b21488 at 1411.256 kb on - strand, within SM_b21488 at 1411.341 kb on - strand at 1411.410 kb on - strand at 1411.550 kb on - strand at 1411.603 kb on + strand, within SM_b21489 at 1411.603 kb on + strand, within SM_b21489 at 1411.604 kb on - strand, within SM_b21489 at 1411.669 kb on + strand, within SM_b21489 at 1411.787 kb on - strand, within SM_b21489 at 1412.105 kb on - strand at 1412.566 kb on + strand at 1412.567 kb on - strand at 1412.632 kb on + strand at 1412.946 kb on + strand, within SM_b21490 at 1413.363 kb on + strand at 1413.364 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 1,411,166 + SM_b21488 0.83 -0.5 1,411,216 + SM_b21488 0.86 +0.8 1,411,256 - SM_b21488 0.88 +0.0 1,411,256 - SM_b21488 0.88 +0.2 1,411,256 - SM_b21488 0.88 +0.7 1,411,341 - -0.2 1,411,410 - +1.1 1,411,550 - -1.8 1,411,603 + SM_b21489 0.17 +0.2 1,411,603 + SM_b21489 0.17 +0.9 1,411,604 - SM_b21489 0.17 +0.6 1,411,669 + SM_b21489 0.27 +0.7 1,411,787 - SM_b21489 0.46 +0.9 1,412,105 - +0.1 1,412,566 + +0.4 1,412,567 - -0.5 1,412,632 + +0.8 1,412,946 + SM_b21490 0.38 -1.1 1,413,363 + -0.4 1,413,364 - +0.3
Or see this region's nucleotide sequence