Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20918 and SM_b20919 overlap by 4 nucleotides SM_b20919 and SM_b20920 overlap by 51 nucleotides SM_b20920 and SM_b20921 are separated by 54 nucleotides SM_b20921 and SM_b20922 overlap by 116 nucleotides
SM_b20918: SM_b20918 - transposase of insertion sequence ISRm1 orfA protein, at 1,325,890 to 1,326,282
_b20918
SM_b20919: SM_b20919 - transposase of insertion sequence ISRm1 orfB protein, at 1,326,279 to 1,327,109
_b20919
SM_b20920: SM_b20920 - hypothetical protein, at 1,327,059 to 1,327,481
_b20920
SM_b20921: SM_b20921 - hypothetical protein, at 1,327,536 to 1,327,778
_b20921
SM_b20922: SM_b20922 - hypothetical protein, at 1,327,663 to 1,328,715
_b20922
Position (kb)
1327
1328 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1327.187 kb on + strand, within SM_b20920 at 1327.191 kb on + strand, within SM_b20920 at 1327.192 kb on - strand, within SM_b20920 at 1327.192 kb on - strand, within SM_b20920 at 1327.192 kb on - strand, within SM_b20920 at 1327.440 kb on - strand at 1327.448 kb on + strand at 1327.449 kb on - strand at 1327.449 kb on - strand at 1327.451 kb on - strand at 1327.522 kb on + strand at 1327.522 kb on + strand at 1327.537 kb on + strand at 1327.618 kb on - strand, within SM_b20921 at 1327.618 kb on - strand, within SM_b20921 at 1327.900 kb on + strand, within SM_b20922 at 1327.901 kb on - strand, within SM_b20922 at 1327.901 kb on - strand, within SM_b20922 at 1328.016 kb on + strand, within SM_b20922 at 1328.016 kb on + strand, within SM_b20922 at 1328.017 kb on - strand, within SM_b20922 at 1328.211 kb on + strand, within SM_b20922 at 1328.212 kb on - strand, within SM_b20922 at 1328.438 kb on + strand, within SM_b20922 at 1328.438 kb on + strand, within SM_b20922 at 1328.439 kb on - strand, within SM_b20922 at 1328.439 kb on - strand, within SM_b20922
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 1,327,187 + SM_b20920 0.30 -1.2 1,327,191 + SM_b20920 0.31 +0.5 1,327,192 - SM_b20920 0.31 -0.5 1,327,192 - SM_b20920 0.31 -1.8 1,327,192 - SM_b20920 0.31 -1.2 1,327,440 - +0.6 1,327,448 + +0.3 1,327,449 - +0.9 1,327,449 - -0.3 1,327,451 - +0.0 1,327,522 + -1.8 1,327,522 + -0.4 1,327,537 + -0.4 1,327,618 - SM_b20921 0.34 -0.2 1,327,618 - SM_b20921 0.34 -0.9 1,327,900 + SM_b20922 0.23 +1.8 1,327,901 - SM_b20922 0.23 -0.5 1,327,901 - SM_b20922 0.23 -0.3 1,328,016 + SM_b20922 0.34 -0.0 1,328,016 + SM_b20922 0.34 +1.8 1,328,017 - SM_b20922 0.34 +0.2 1,328,211 + SM_b20922 0.52 +0.5 1,328,212 - SM_b20922 0.52 +0.1 1,328,438 + SM_b20922 0.74 -0.5 1,328,438 + SM_b20922 0.74 -1.0 1,328,439 - SM_b20922 0.74 +1.6 1,328,439 - SM_b20922 0.74 -0.0
Or see this region's nucleotide sequence