Experiment: L-Histidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20045 and SM_b20046 are separated by 3 nucleotides SM_b20046 and SM_b20047 are separated by 366 nucleotides SM_b20047 and SM_b20048 are separated by 168 nucleotides
SM_b20045: SM_b20045 - replication protein B, at 56,556 to 57,560
_b20045
SM_b20046: SM_b20046 - replication protein A, at 57,564 to 58,760
_b20046
SM_b20047: SM_b20047 - hypothetical protein, at 59,127 to 59,315
_b20047
SM_b20048: SM_b20048 - transcriptional regulator, at 59,484 to 60,191
_b20048
Position (kb) 57
58
59 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 58.816 kb on - strand at 59.042 kb on - strand at 59.111 kb on - strand at 59.122 kb on - strand at 59.134 kb on - strand at 59.134 kb on - strand at 59.310 kb on + strand at 59.310 kb on + strand at 59.310 kb on + strand at 59.310 kb on + strand at 59.310 kb on + strand at 59.311 kb on - strand at 59.311 kb on - strand at 59.405 kb on + strand at 59.406 kb on - strand at 59.454 kb on + strand at 59.454 kb on + strand at 59.455 kb on - strand at 59.677 kb on - strand, within SM_b20048 at 59.677 kb on - strand, within SM_b20048 at 59.677 kb on - strand at 59.677 kb on - strand, within SM_b20048 at 59.677 kb on - strand, within SM_b20048 at 59.677 kb on - strand, within SM_b20048 at 59.738 kb on - strand, within SM_b20048 at 59.738 kb on - strand, within SM_b20048 at 59.738 kb on - strand, within SM_b20048 at 59.738 kb on - strand, within SM_b20048 at 59.738 kb on - strand, within SM_b20048 at 59.738 kb on - strand, within SM_b20048 at 59.757 kb on + strand, within SM_b20048 at 59.758 kb on - strand, within SM_b20048
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine remove 58,816 - +3.2 59,042 - -1.6 59,111 - +0.2 59,122 - +0.5 59,134 - -0.2 59,134 - +0.5 59,310 + -0.4 59,310 + +0.9 59,310 + +0.5 59,310 + -0.6 59,310 + +0.4 59,311 - +0.2 59,311 - +0.9 59,405 + +0.1 59,406 - -2.1 59,454 + -1.9 59,454 + -2.5 59,455 - -2.2 59,677 - SM_b20048 0.27 -1.7 59,677 - SM_b20048 0.27 -2.1 59,677 - +1.9 59,677 - SM_b20048 0.27 -2.8 59,677 - SM_b20048 0.27 -0.7 59,677 - SM_b20048 0.27 -1.3 59,738 - SM_b20048 0.36 -2.7 59,738 - SM_b20048 0.36 -1.1 59,738 - SM_b20048 0.36 -1.4 59,738 - SM_b20048 0.36 -1.9 59,738 - SM_b20048 0.36 -1.7 59,738 - SM_b20048 0.36 -1.6 59,757 + SM_b20048 0.39 -2.4 59,758 - SM_b20048 0.39 -1.5
Or see this region's nucleotide sequence