Experiment: bromate 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2859 and Pf6N2E2_2860 are separated by 80 nucleotides Pf6N2E2_2860 and Pf6N2E2_2861 are separated by 32 nucleotides Pf6N2E2_2861 and Pf6N2E2_2862 overlap by 12 nucleotides
Pf6N2E2_2859 - Bacterial transcription activator, effector binding protein, at 3,349,889 to 3,350,377
_2859
Pf6N2E2_2860 - Putative threonine efflux protein, at 3,350,458 to 3,351,120
_2860
Pf6N2E2_2861 - hypothetical protein, at 3,351,153 to 3,351,341
_2861
Pf6N2E2_2862 - Uncharacterized PLP-dependent aminotransferase YfdZ, at 3,351,330 to 3,352,547
_2862
Position (kb)
3351
3352 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3350.157 kb on + strand, within Pf6N2E2_2859 at 3350.157 kb on + strand, within Pf6N2E2_2859 at 3350.158 kb on - strand, within Pf6N2E2_2859 at 3350.199 kb on + strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859 at 3350.314 kb on + strand, within Pf6N2E2_2859 at 3350.355 kb on + strand at 3350.420 kb on + strand at 3350.420 kb on + strand at 3350.420 kb on + strand at 3350.421 kb on - strand at 3350.421 kb on - strand at 3350.421 kb on - strand at 3350.421 kb on - strand at 3350.421 kb on - strand at 3350.421 kb on - strand at 3350.494 kb on + strand at 3350.526 kb on - strand, within Pf6N2E2_2860 at 3350.712 kb on + strand, within Pf6N2E2_2860 at 3350.713 kb on - strand, within Pf6N2E2_2860 at 3350.718 kb on + strand, within Pf6N2E2_2860 at 3350.847 kb on - strand, within Pf6N2E2_2860 at 3350.879 kb on + strand, within Pf6N2E2_2860 at 3350.879 kb on + strand, within Pf6N2E2_2860 at 3350.880 kb on - strand, within Pf6N2E2_2860 at 3350.930 kb on + strand, within Pf6N2E2_2860 at 3350.931 kb on - strand, within Pf6N2E2_2860 at 3350.973 kb on - strand, within Pf6N2E2_2860 at 3351.070 kb on + strand at 3351.070 kb on + strand at 3351.070 kb on + strand at 3351.071 kb on - strand at 3351.071 kb on - strand at 3351.071 kb on - strand at 3351.121 kb on + strand at 3351.122 kb on - strand at 3351.143 kb on + strand at 3351.213 kb on + strand, within Pf6N2E2_2861 at 3351.255 kb on - strand, within Pf6N2E2_2861 at 3351.315 kb on + strand, within Pf6N2E2_2861 at 3351.315 kb on + strand, within Pf6N2E2_2861 at 3351.315 kb on + strand, within Pf6N2E2_2861 at 3351.316 kb on - strand, within Pf6N2E2_2861 at 3351.316 kb on - strand, within Pf6N2E2_2861 at 3351.316 kb on - strand, within Pf6N2E2_2861 at 3351.316 kb on - strand, within Pf6N2E2_2861 at 3351.316 kb on - strand, within Pf6N2E2_2861 at 3351.384 kb on + strand at 3351.385 kb on - strand at 3351.388 kb on + strand at 3351.388 kb on + strand at 3351.388 kb on + strand at 3351.389 kb on - strand at 3351.389 kb on - strand at 3351.389 kb on - strand at 3351.396 kb on - strand at 3351.589 kb on - strand, within Pf6N2E2_2862 at 3351.601 kb on - strand, within Pf6N2E2_2862 at 3351.601 kb on - strand, within Pf6N2E2_2862 at 3351.738 kb on + strand, within Pf6N2E2_2862 at 3351.739 kb on - strand, within Pf6N2E2_2862 at 3351.739 kb on - strand, within Pf6N2E2_2862 at 3351.739 kb on - strand, within Pf6N2E2_2862 at 3351.936 kb on + strand, within Pf6N2E2_2862 at 3351.937 kb on - strand, within Pf6N2E2_2862 at 3352.065 kb on + strand, within Pf6N2E2_2862 at 3352.065 kb on + strand, within Pf6N2E2_2862 at 3352.066 kb on - strand, within Pf6N2E2_2862 at 3352.066 kb on - strand, within Pf6N2E2_2862 at 3352.143 kb on + strand, within Pf6N2E2_2862 at 3352.143 kb on + strand, within Pf6N2E2_2862 at 3352.143 kb on + strand, within Pf6N2E2_2862 at 3352.144 kb on - strand, within Pf6N2E2_2862 at 3352.152 kb on + strand, within Pf6N2E2_2862 at 3352.255 kb on + strand, within Pf6N2E2_2862 at 3352.314 kb on + strand, within Pf6N2E2_2862 at 3352.314 kb on + strand, within Pf6N2E2_2862 at 3352.314 kb on + strand, within Pf6N2E2_2862 at 3352.314 kb on + strand, within Pf6N2E2_2862 at 3352.314 kb on + strand, within Pf6N2E2_2862 at 3352.314 kb on + strand, within Pf6N2E2_2862 at 3352.314 kb on + strand, within Pf6N2E2_2862 at 3352.315 kb on - strand, within Pf6N2E2_2862 at 3352.341 kb on + strand, within Pf6N2E2_2862 at 3352.341 kb on + strand, within Pf6N2E2_2862 at 3352.341 kb on + strand, within Pf6N2E2_2862
Per-strain Table
Position Strand Gene LocusTag Fraction bromate 0.05 M remove 3,350,157 + Pf6N2E2_2859 0.55 +0.2 3,350,157 + Pf6N2E2_2859 0.55 +0.6 3,350,158 - Pf6N2E2_2859 0.55 +1.6 3,350,199 + Pf6N2E2_2859 0.63 +0.1 3,350,200 - Pf6N2E2_2859 0.64 -0.4 3,350,200 - Pf6N2E2_2859 0.64 -0.7 3,350,200 - Pf6N2E2_2859 0.64 -1.7 3,350,200 - Pf6N2E2_2859 0.64 -1.2 3,350,314 + Pf6N2E2_2859 0.87 -0.3 3,350,355 + +0.7 3,350,420 + +0.8 3,350,420 + +0.1 3,350,420 + +0.5 3,350,421 - +0.4 3,350,421 - -0.3 3,350,421 - +0.1 3,350,421 - -0.6 3,350,421 - -0.2 3,350,421 - -0.6 3,350,494 + -1.3 3,350,526 - Pf6N2E2_2860 0.10 +0.4 3,350,712 + Pf6N2E2_2860 0.38 +0.4 3,350,713 - Pf6N2E2_2860 0.38 +0.4 3,350,718 + Pf6N2E2_2860 0.39 +1.0 3,350,847 - Pf6N2E2_2860 0.59 -1.0 3,350,879 + Pf6N2E2_2860 0.63 +1.4 3,350,879 + Pf6N2E2_2860 0.63 -2.9 3,350,880 - Pf6N2E2_2860 0.64 -1.6 3,350,930 + Pf6N2E2_2860 0.71 -1.6 3,350,931 - Pf6N2E2_2860 0.71 +0.5 3,350,973 - Pf6N2E2_2860 0.78 -3.0 3,351,070 + -0.1 3,351,070 + -0.3 3,351,070 + +1.0 3,351,071 - +1.2 3,351,071 - -0.4 3,351,071 - -0.1 3,351,121 + +0.6 3,351,122 - -0.5 3,351,143 + -0.3 3,351,213 + Pf6N2E2_2861 0.32 -0.1 3,351,255 - Pf6N2E2_2861 0.54 -1.3 3,351,315 + Pf6N2E2_2861 0.86 +0.4 3,351,315 + Pf6N2E2_2861 0.86 +0.1 3,351,315 + Pf6N2E2_2861 0.86 -1.6 3,351,316 - Pf6N2E2_2861 0.86 -0.6 3,351,316 - Pf6N2E2_2861 0.86 -1.5 3,351,316 - Pf6N2E2_2861 0.86 -1.1 3,351,316 - Pf6N2E2_2861 0.86 +0.9 3,351,316 - Pf6N2E2_2861 0.86 -0.3 3,351,384 + -0.7 3,351,385 - -0.9 3,351,388 + +0.4 3,351,388 + +0.5 3,351,388 + +0.5 3,351,389 - -0.9 3,351,389 - +2.9 3,351,389 - +0.4 3,351,396 - +0.6 3,351,589 - Pf6N2E2_2862 0.21 -0.7 3,351,601 - Pf6N2E2_2862 0.22 -0.0 3,351,601 - Pf6N2E2_2862 0.22 -0.9 3,351,738 + Pf6N2E2_2862 0.33 +0.6 3,351,739 - Pf6N2E2_2862 0.34 +0.9 3,351,739 - Pf6N2E2_2862 0.34 +0.1 3,351,739 - Pf6N2E2_2862 0.34 +0.1 3,351,936 + Pf6N2E2_2862 0.50 -1.4 3,351,937 - Pf6N2E2_2862 0.50 +1.3 3,352,065 + Pf6N2E2_2862 0.60 -1.4 3,352,065 + Pf6N2E2_2862 0.60 +1.4 3,352,066 - Pf6N2E2_2862 0.60 -0.7 3,352,066 - Pf6N2E2_2862 0.60 +1.2 3,352,143 + Pf6N2E2_2862 0.67 +1.1 3,352,143 + Pf6N2E2_2862 0.67 -0.4 3,352,143 + Pf6N2E2_2862 0.67 -0.1 3,352,144 - Pf6N2E2_2862 0.67 +0.4 3,352,152 + Pf6N2E2_2862 0.67 +1.7 3,352,255 + Pf6N2E2_2862 0.76 +0.8 3,352,314 + Pf6N2E2_2862 0.81 -1.1 3,352,314 + Pf6N2E2_2862 0.81 +0.4 3,352,314 + Pf6N2E2_2862 0.81 -0.6 3,352,314 + Pf6N2E2_2862 0.81 -0.8 3,352,314 + Pf6N2E2_2862 0.81 +1.1 3,352,314 + Pf6N2E2_2862 0.81 +1.2 3,352,314 + Pf6N2E2_2862 0.81 +1.3 3,352,315 - Pf6N2E2_2862 0.81 +2.0 3,352,341 + Pf6N2E2_2862 0.83 +0.1 3,352,341 + Pf6N2E2_2862 0.83 +1.3 3,352,341 + Pf6N2E2_2862 0.83 -2.1
Or see this region's nucleotide sequence