Experiment: bromate 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1309 and Pf6N2E2_1310 are separated by 13 nucleotides Pf6N2E2_1310 and Pf6N2E2_1311 are separated by 91 nucleotides Pf6N2E2_1311 and Pf6N2E2_1312 are separated by 52 nucleotides
Pf6N2E2_1309 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 1,542,921 to 1,544,381
_1309
Pf6N2E2_1310 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase (EC 1.13.11.15), at 1,544,395 to 1,545,426
_1310
Pf6N2E2_1311 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 1,545,518 to 1,545,910
_1311
Pf6N2E2_1312 - 4-hydroxyphenylacetate symporter, major facilitator superfamily (MFS), at 1,545,963 to 1,547,267
_1312
Position (kb)
1544
1545
1546 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1543.501 kb on + strand, within Pf6N2E2_1309 at 1543.565 kb on - strand, within Pf6N2E2_1309 at 1543.908 kb on + strand, within Pf6N2E2_1309 at 1544.136 kb on + strand, within Pf6N2E2_1309 at 1544.151 kb on + strand, within Pf6N2E2_1309 at 1544.152 kb on - strand, within Pf6N2E2_1309 at 1544.152 kb on - strand, within Pf6N2E2_1309 at 1544.301 kb on + strand at 1544.362 kb on + strand at 1544.362 kb on + strand at 1544.389 kb on + strand at 1544.390 kb on - strand at 1544.617 kb on + strand, within Pf6N2E2_1310 at 1544.617 kb on + strand, within Pf6N2E2_1310 at 1544.617 kb on + strand, within Pf6N2E2_1310 at 1544.618 kb on - strand, within Pf6N2E2_1310 at 1544.618 kb on - strand, within Pf6N2E2_1310 at 1544.794 kb on + strand, within Pf6N2E2_1310 at 1544.794 kb on + strand, within Pf6N2E2_1310 at 1544.795 kb on - strand, within Pf6N2E2_1310 at 1544.795 kb on - strand, within Pf6N2E2_1310 at 1544.795 kb on - strand, within Pf6N2E2_1310 at 1544.837 kb on - strand, within Pf6N2E2_1310 at 1544.837 kb on - strand, within Pf6N2E2_1310 at 1544.837 kb on - strand, within Pf6N2E2_1310 at 1544.842 kb on + strand, within Pf6N2E2_1310 at 1544.965 kb on + strand, within Pf6N2E2_1310 at 1544.966 kb on - strand, within Pf6N2E2_1310 at 1544.966 kb on - strand, within Pf6N2E2_1310 at 1545.347 kb on - strand at 1545.450 kb on - strand at 1545.450 kb on - strand at 1545.480 kb on + strand at 1545.703 kb on - strand, within Pf6N2E2_1311 at 1545.703 kb on - strand, within Pf6N2E2_1311 at 1546.063 kb on - strand at 1546.311 kb on + strand, within Pf6N2E2_1312
Per-strain Table
Position Strand Gene LocusTag Fraction bromate 0.05 M remove 1,543,501 + Pf6N2E2_1309 0.40 -0.1 1,543,565 - Pf6N2E2_1309 0.44 +0.7 1,543,908 + Pf6N2E2_1309 0.68 -2.4 1,544,136 + Pf6N2E2_1309 0.83 -2.8 1,544,151 + Pf6N2E2_1309 0.84 +1.4 1,544,152 - Pf6N2E2_1309 0.84 +1.3 1,544,152 - Pf6N2E2_1309 0.84 -0.3 1,544,301 + -0.5 1,544,362 + +0.2 1,544,362 + +0.0 1,544,389 + -0.2 1,544,390 - -0.0 1,544,617 + Pf6N2E2_1310 0.22 +1.8 1,544,617 + Pf6N2E2_1310 0.22 -0.4 1,544,617 + Pf6N2E2_1310 0.22 +1.2 1,544,618 - Pf6N2E2_1310 0.22 +0.1 1,544,618 - Pf6N2E2_1310 0.22 +0.0 1,544,794 + Pf6N2E2_1310 0.39 -0.4 1,544,794 + Pf6N2E2_1310 0.39 -0.4 1,544,795 - Pf6N2E2_1310 0.39 +0.9 1,544,795 - Pf6N2E2_1310 0.39 +0.2 1,544,795 - Pf6N2E2_1310 0.39 +0.3 1,544,837 - Pf6N2E2_1310 0.43 +0.4 1,544,837 - Pf6N2E2_1310 0.43 +0.9 1,544,837 - Pf6N2E2_1310 0.43 +0.3 1,544,842 + Pf6N2E2_1310 0.43 +2.1 1,544,965 + Pf6N2E2_1310 0.55 +0.2 1,544,966 - Pf6N2E2_1310 0.55 -0.1 1,544,966 - Pf6N2E2_1310 0.55 +0.4 1,545,347 - -0.8 1,545,450 - -1.8 1,545,450 - -0.2 1,545,480 + +0.8 1,545,703 - Pf6N2E2_1311 0.47 +2.0 1,545,703 - Pf6N2E2_1311 0.47 +0.4 1,546,063 - +1.1 1,546,311 + Pf6N2E2_1312 0.27 -0.8
Or see this region's nucleotide sequence