Experiment: Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3298 and Shew_3299 are separated by 15 nucleotides Shew_3299 and Shew_3300 are separated by 15 nucleotides Shew_3300 and Shew_3301 overlap by 1 nucleotides Shew_3301 and Shew_3302 are separated by 11 nucleotides Shew_3302 and Shew_3303 are separated by 71 nucleotides
Shew_3298: Shew_3298 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (RefSeq), at 3,942,260 to 3,943,519
_3298
Shew_3299: Shew_3299 - BolA family protein (RefSeq), at 3,943,535 to 3,943,786
_3299
Shew_3300: Shew_3300 - anti-sigma-factor antagonist (RefSeq), at 3,943,802 to 3,944,095
_3300
Shew_3301: Shew_3301 - toluene tolerance family protein (RefSeq), at 3,944,095 to 3,944,748
_3301
Shew_3302: Shew_3302 - hypothetical protein (RefSeq), at 3,944,760 to 3,945,233
_3302
Shew_3303: Shew_3303 - hypothetical protein (RefSeq), at 3,945,305 to 3,946,090
_3303
Position (kb)
3944
3945 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3944.446 kb on - strand, within Shew_3301 at 3944.513 kb on - strand, within Shew_3301 at 3944.569 kb on - strand, within Shew_3301 at 3944.579 kb on - strand, within Shew_3301 at 3944.669 kb on + strand, within Shew_3301 at 3944.901 kb on + strand, within Shew_3302 at 3945.133 kb on - strand, within Shew_3302 at 3945.250 kb on + strand at 3945.250 kb on + strand at 3945.250 kb on + strand at 3945.258 kb on - strand at 3945.258 kb on - strand at 3945.258 kb on - strand at 3945.258 kb on - strand at 3945.258 kb on - strand at 3945.258 kb on - strand at 3945.258 kb on - strand at 3945.439 kb on - strand, within Shew_3303 at 3945.439 kb on - strand, within Shew_3303 at 3945.483 kb on - strand, within Shew_3303 at 3945.491 kb on - strand, within Shew_3303 at 3945.518 kb on + strand, within Shew_3303 at 3945.518 kb on - strand, within Shew_3303 at 3945.639 kb on + strand, within Shew_3303 at 3945.647 kb on - strand, within Shew_3303
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.03125 mg/ml remove 3,944,446 - Shew_3301 0.54 -2.0 3,944,513 - Shew_3301 0.64 -1.5 3,944,569 - Shew_3301 0.72 -2.4 3,944,579 - Shew_3301 0.74 -2.2 3,944,669 + Shew_3301 0.88 -0.9 3,944,901 + Shew_3302 0.30 +0.3 3,945,133 - Shew_3302 0.79 -3.4 3,945,250 + +2.5 3,945,250 + -0.5 3,945,250 + -1.2 3,945,258 - -1.9 3,945,258 - -0.0 3,945,258 - -0.5 3,945,258 - -0.2 3,945,258 - -0.1 3,945,258 - -0.0 3,945,258 - -0.5 3,945,439 - Shew_3303 0.17 -2.3 3,945,439 - Shew_3303 0.17 -1.9 3,945,483 - Shew_3303 0.23 -1.8 3,945,491 - Shew_3303 0.24 -1.9 3,945,518 + Shew_3303 0.27 -0.9 3,945,518 - Shew_3303 0.27 -2.0 3,945,639 + Shew_3303 0.42 -0.3 3,945,647 - Shew_3303 0.44 -1.6
Or see this region's nucleotide sequence