Experiment: Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2358 and Shew_2359 are separated by 69 nucleotides Shew_2359 and Shew_2360 are separated by 161 nucleotides Shew_2360 and Shew_2361 overlap by 4 nucleotides
Shew_2358: Shew_2358 - peptidase M24 (RefSeq), at 2,758,272 to 2,759,591
_2358
Shew_2359: Shew_2359 - uracil-DNA glycosylase superfamily protein (RefSeq), at 2,759,661 to 2,760,251
_2359
Shew_2360: Shew_2360 - D-hydroxyproline dehydrogenase (from data) , at 2,760,413 to 2,761,753
_2360
Shew_2361: Shew_2361 - proline racemase (RefSeq), at 2,761,750 to 2,762,790
_2361
Position (kb)
2760
2761
2762 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2759.728 kb on + strand, within Shew_2359 at 2760.280 kb on + strand at 2760.582 kb on + strand, within Shew_2360 at 2760.606 kb on - strand, within Shew_2360 at 2760.651 kb on + strand, within Shew_2360 at 2760.685 kb on + strand, within Shew_2360 at 2760.995 kb on - strand, within Shew_2360 at 2761.155 kb on + strand, within Shew_2360 at 2761.181 kb on + strand, within Shew_2360 at 2761.461 kb on - strand, within Shew_2360 at 2761.701 kb on + strand at 2761.701 kb on + strand at 2762.211 kb on - strand, within Shew_2361 at 2762.366 kb on + strand, within Shew_2361 at 2762.407 kb on + strand, within Shew_2361 at 2762.433 kb on + strand, within Shew_2361
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.03125 mg/ml remove 2,759,728 + Shew_2359 0.11 +0.2 2,760,280 + -0.1 2,760,582 + Shew_2360 0.13 +0.3 2,760,606 - Shew_2360 0.14 +0.3 2,760,651 + Shew_2360 0.18 +0.1 2,760,685 + Shew_2360 0.20 +0.2 2,760,995 - Shew_2360 0.43 -0.6 2,761,155 + Shew_2360 0.55 -1.1 2,761,181 + Shew_2360 0.57 +0.8 2,761,461 - Shew_2360 0.78 -0.1 2,761,701 + -0.0 2,761,701 + -0.3 2,762,211 - Shew_2361 0.44 +4.1 2,762,366 + Shew_2361 0.59 -0.1 2,762,407 + Shew_2361 0.63 -0.6 2,762,433 + Shew_2361 0.66 -0.2
Or see this region's nucleotide sequence