Experiment: Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1756 and Shew_1757 are separated by 2 nucleotides Shew_1757 and Shew_1758 overlap by 4 nucleotides Shew_1758 and Shew_1759 are separated by 2 nucleotides
Shew_1756: Shew_1756 - hydrogenase expression/formation protein HypE (RefSeq), at 2,006,941 to 2,007,951
_1756
Shew_1757: Shew_1757 - hydrogenase expression/formation protein HypD (RefSeq), at 2,007,954 to 2,009,069
_1757
Shew_1758: Shew_1758 - hydrogenase assembly chaperone hypC/hupF (RefSeq), at 2,009,066 to 2,009,314
_1758
Shew_1759: Shew_1759 - hydrogenase accessory protein HypB (RefSeq), at 2,009,317 to 2,010,150
_1759
Position (kb)
2007
2008
2009
2010 Strain fitness (log2 ratio)
-1
0
1 at 2006.968 kb on - strand at 2007.120 kb on + strand, within Shew_1756 at 2007.195 kb on - strand, within Shew_1756 at 2007.969 kb on - strand at 2008.316 kb on - strand, within Shew_1757 at 2008.346 kb on + strand, within Shew_1757 at 2009.124 kb on - strand, within Shew_1758 at 2009.148 kb on + strand, within Shew_1758 at 2009.414 kb on + strand, within Shew_1759 at 2009.551 kb on + strand, within Shew_1759 at 2009.561 kb on + strand, within Shew_1759 at 2009.569 kb on - strand, within Shew_1759 at 2009.632 kb on + strand, within Shew_1759 at 2009.637 kb on + strand, within Shew_1759 at 2010.021 kb on + strand, within Shew_1759 at 2010.021 kb on + strand, within Shew_1759
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.03125 mg/ml remove 2,006,968 - +0.4 2,007,120 + Shew_1756 0.18 +0.0 2,007,195 - Shew_1756 0.25 -0.1 2,007,969 - -0.4 2,008,316 - Shew_1757 0.32 -0.4 2,008,346 + Shew_1757 0.35 -0.5 2,009,124 - Shew_1758 0.23 +0.0 2,009,148 + Shew_1758 0.33 -0.0 2,009,414 + Shew_1759 0.12 -0.3 2,009,551 + Shew_1759 0.28 -0.8 2,009,561 + Shew_1759 0.29 +0.1 2,009,569 - Shew_1759 0.30 +0.1 2,009,632 + Shew_1759 0.38 -0.2 2,009,637 + Shew_1759 0.38 +0.1 2,010,021 + Shew_1759 0.84 -0.4 2,010,021 + Shew_1759 0.84 -0.0
Or see this region's nucleotide sequence