Experiment: Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1470 and Shew_1471 are separated by 100 nucleotides Shew_1471 and Shew_1472 are separated by 77 nucleotides Shew_1472 and Shew_1473 are separated by 229 nucleotides
Shew_1470: Shew_1470 - sulfatase (RefSeq), at 1,695,404 to 1,697,191
_1470
Shew_1471: Shew_1471 - hypothetical protein (RefSeq), at 1,697,292 to 1,697,507
_1471
Shew_1472: Shew_1472 - nucleoid-associated protein NdpA (RefSeq), at 1,697,585 to 1,698,616
_1472
Shew_1473: Shew_1473 - hypothetical protein (RefSeq), at 1,698,846 to 1,699,730
_1473
Position (kb)
1697
1698
1699 Strain fitness (log2 ratio)
-2
-1
0
1 at 1696.689 kb on - strand, within Shew_1470 at 1697.394 kb on + strand, within Shew_1471 at 1697.458 kb on - strand, within Shew_1471 at 1697.587 kb on - strand at 1697.649 kb on + strand at 1697.649 kb on + strand at 1697.657 kb on - strand at 1697.657 kb on - strand at 1697.657 kb on - strand at 1697.968 kb on + strand, within Shew_1472 at 1698.001 kb on - strand, within Shew_1472 at 1698.250 kb on + strand, within Shew_1472 at 1698.258 kb on - strand, within Shew_1472 at 1698.331 kb on + strand, within Shew_1472 at 1698.663 kb on - strand at 1698.707 kb on - strand at 1698.723 kb on - strand at 1698.723 kb on - strand at 1699.065 kb on - strand, within Shew_1473 at 1699.072 kb on - strand, within Shew_1473 at 1699.133 kb on + strand, within Shew_1473 at 1699.143 kb on + strand, within Shew_1473 at 1699.150 kb on + strand, within Shew_1473 at 1699.197 kb on - strand, within Shew_1473 at 1699.502 kb on - strand, within Shew_1473
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.03125 mg/ml remove 1,696,689 - Shew_1470 0.72 -2.6 1,697,394 + Shew_1471 0.47 -1.3 1,697,458 - Shew_1471 0.77 -0.4 1,697,587 - -0.6 1,697,649 + -1.0 1,697,649 + -0.4 1,697,657 - -0.3 1,697,657 - -1.1 1,697,657 - -0.5 1,697,968 + Shew_1472 0.37 -0.7 1,698,001 - Shew_1472 0.40 -0.3 1,698,250 + Shew_1472 0.64 -1.2 1,698,258 - Shew_1472 0.65 -0.1 1,698,331 + Shew_1472 0.72 -0.6 1,698,663 - +0.6 1,698,707 - -0.2 1,698,723 - -0.1 1,698,723 - -0.1 1,699,065 - Shew_1473 0.25 +0.0 1,699,072 - Shew_1473 0.26 +0.3 1,699,133 + Shew_1473 0.32 +0.4 1,699,143 + Shew_1473 0.34 +0.6 1,699,150 + Shew_1473 0.34 -0.1 1,699,197 - Shew_1473 0.40 -0.1 1,699,502 - Shew_1473 0.74 +0.6
Or see this region's nucleotide sequence