Experiment: Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1431 and Shew_1432 are separated by 110 nucleotides Shew_1432 and Shew_1433 are separated by 44 nucleotides Shew_1433 and Shew_1434 overlap by 1 nucleotides
Shew_1431: Shew_1431 - hypothetical protein (RefSeq), at 1,646,533 to 1,646,988
_1431
Shew_1432: Shew_1432 - ferredoxin-type protein NapF (RefSeq), at 1,647,099 to 1,647,575
_1432
Shew_1433: Shew_1433 - UDP-glucose 4-epimerase (RefSeq), at 1,647,620 to 1,648,633
_1433
Shew_1434: Shew_1434 - UTP-glucose-1-phosphate uridylyltransferase GalU (RefSeq), at 1,648,633 to 1,649,553
_1434
Position (kb)
1647
1648
1649 Strain fitness (log2 ratio)
-1
0
1 at 1646.698 kb on + strand, within Shew_1431 at 1646.901 kb on - strand, within Shew_1431 at 1646.901 kb on - strand, within Shew_1431 at 1647.279 kb on - strand, within Shew_1432 at 1647.640 kb on + strand at 1647.759 kb on + strand, within Shew_1433 at 1647.834 kb on + strand, within Shew_1433 at 1647.847 kb on - strand, within Shew_1433 at 1648.313 kb on - strand, within Shew_1433 at 1648.315 kb on + strand, within Shew_1433 at 1648.315 kb on + strand, within Shew_1433 at 1648.323 kb on - strand, within Shew_1433 at 1648.585 kb on - strand at 1648.637 kb on + strand at 1649.220 kb on - strand, within Shew_1434
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.03125 mg/ml remove 1,646,698 + Shew_1431 0.36 +1.2 1,646,901 - Shew_1431 0.81 -1.5 1,646,901 - Shew_1431 0.81 +0.3 1,647,279 - Shew_1432 0.38 +0.4 1,647,640 + -0.5 1,647,759 + Shew_1433 0.14 -0.7 1,647,834 + Shew_1433 0.21 -0.7 1,647,847 - Shew_1433 0.22 -1.1 1,648,313 - Shew_1433 0.68 -0.7 1,648,315 + Shew_1433 0.69 -1.7 1,648,315 + Shew_1433 0.69 -0.7 1,648,323 - Shew_1433 0.69 -0.8 1,648,585 - -0.9 1,648,637 + -0.1 1,649,220 - Shew_1434 0.64 -0.3
Or see this region's nucleotide sequence