Experiment: Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trmB and Shew_1125 are separated by 30 nucleotides Shew_1125 and Shew_1126 overlap by 1 nucleotides Shew_1126 and Shew_1127 are separated by 299 nucleotides
Shew_1124: trmB - tRNA (guanine-N(7))-methyltransferase (RefSeq), at 1,299,395 to 1,300,111
trmB
Shew_1125: Shew_1125 - hypothetical protein (RefSeq), at 1,300,142 to 1,300,465
_1125
Shew_1126: Shew_1126 - glutaminase (RefSeq), at 1,300,465 to 1,301,379
_1126
Shew_1127: Shew_1127 - hypothetical protein (RefSeq), at 1,301,679 to 1,302,509
_1127
Position (kb)
1300
1301
1302 Strain fitness (log2 ratio)
-1
0
1 at 1299.740 kb on - strand, within trmB at 1299.801 kb on + strand, within trmB at 1300.247 kb on - strand, within Shew_1125 at 1301.179 kb on + strand, within Shew_1126 at 1301.179 kb on - strand, within Shew_1126 at 1301.184 kb on + strand, within Shew_1126 at 1301.356 kb on + strand at 1301.356 kb on + strand at 1301.356 kb on + strand at 1301.359 kb on - strand at 1301.428 kb on + strand at 1301.446 kb on - strand at 1301.528 kb on + strand at 1301.638 kb on - strand at 1301.638 kb on - strand at 1301.772 kb on - strand, within Shew_1127 at 1301.964 kb on - strand, within Shew_1127
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.03125 mg/ml remove 1,299,740 - trmB Shew_1124 0.48 -0.1 1,299,801 + trmB Shew_1124 0.57 -0.0 1,300,247 - Shew_1125 0.32 +0.2 1,301,179 + Shew_1126 0.78 -1.7 1,301,179 - Shew_1126 0.78 -0.0 1,301,184 + Shew_1126 0.79 -0.2 1,301,356 + +0.1 1,301,356 + +0.2 1,301,356 + +0.1 1,301,359 - -0.0 1,301,428 + -0.1 1,301,446 - -0.4 1,301,528 + +0.1 1,301,638 - -0.1 1,301,638 - -0.6 1,301,772 - Shew_1127 0.11 +0.5 1,301,964 - Shew_1127 0.34 +0.2
Or see this region's nucleotide sequence