Experiment: Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0623 and Shew_0624 are separated by 70 nucleotides Shew_0624 and Shew_0625 are separated by 90 nucleotides Shew_0625 and Shew_0626 are separated by 13 nucleotides Shew_0626 and Shew_0627 are separated by 11 nucleotides
Shew_0623: Shew_0623 - phage shock protein A, PspA (RefSeq), at 707,888 to 708,574
_0623
Shew_0624: Shew_0624 - hypothetical protein (RefSeq), at 708,645 to 709,058
_0624
Shew_0625: Shew_0625 - spermidine synthase (RefSeq), at 709,149 to 710,876
_0625
Shew_0626: Shew_0626 - hypothetical protein (RefSeq), at 710,890 to 711,789
_0626
Shew_0627: Shew_0627 - hypothetical protein (RefSeq), at 711,801 to 712,757
_0627
Position (kb) 709
710
711 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 708.203 kb on - strand, within Shew_0623 at 708.685 kb on - strand at 709.233 kb on + strand at 709.289 kb on + strand at 709.289 kb on + strand at 709.289 kb on + strand at 709.297 kb on - strand at 709.300 kb on + strand at 709.448 kb on + strand, within Shew_0625 at 709.496 kb on - strand, within Shew_0625 at 709.607 kb on + strand, within Shew_0625 at 709.703 kb on - strand, within Shew_0625 at 709.822 kb on + strand, within Shew_0625 at 709.841 kb on + strand, within Shew_0625 at 710.222 kb on + strand, within Shew_0625 at 710.222 kb on + strand, within Shew_0625 at 710.543 kb on + strand, within Shew_0625 at 710.880 kb on + strand at 710.991 kb on + strand, within Shew_0626 at 711.036 kb on + strand, within Shew_0626 at 711.835 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.03125 mg/ml remove 708,203 - Shew_0623 0.46 -3.0 708,685 - -0.6 709,233 + +1.2 709,289 + +1.1 709,289 + +1.0 709,289 + +0.9 709,297 - +1.0 709,300 + +1.1 709,448 + Shew_0625 0.17 +0.9 709,496 - Shew_0625 0.20 +0.7 709,607 + Shew_0625 0.27 +1.0 709,703 - Shew_0625 0.32 +1.0 709,822 + Shew_0625 0.39 +1.0 709,841 + Shew_0625 0.40 +1.2 710,222 + Shew_0625 0.62 +0.9 710,222 + Shew_0625 0.62 +1.2 710,543 + Shew_0625 0.81 +0.9 710,880 + +0.8 710,991 + Shew_0626 0.11 +0.9 711,036 + Shew_0626 0.16 +1.0 711,835 - +1.3
Or see this region's nucleotide sequence