Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3011 and Dshi_3012 are separated by 117 nucleotides Dshi_3012 and Dshi_3013 are separated by 175 nucleotides Dshi_3013 and Dshi_3014 are separated by 9 nucleotides
Dshi_3011: Dshi_3011 - extracellular solute-binding protein family 5 (RefSeq), at 3,179,758 to 3,181,491
_3011
Dshi_3012: Dshi_3012 - Zn-dependent hydrolase of the beta-lactamase fold (RefSeq), at 3,181,609 to 3,182,298
_3012
Dshi_3013: Dshi_3013 - protein of unknown function DUF606 (RefSeq), at 3,182,474 to 3,182,914
_3013
Dshi_3014: Dshi_3014 - Phytanoyl-CoA dioxygenase (RefSeq), at 3,182,924 to 3,183,751
_3014
Position (kb)
3181
3182
3183 Strain fitness (log2 ratio)
-1
0
1
2 at 3180.629 kb on + strand, within Dshi_3011 at 3180.799 kb on - strand, within Dshi_3011 at 3180.801 kb on + strand, within Dshi_3011 at 3180.809 kb on - strand, within Dshi_3011 at 3180.913 kb on + strand, within Dshi_3011 at 3180.919 kb on + strand, within Dshi_3011 at 3180.946 kb on - strand, within Dshi_3011 at 3180.946 kb on - strand, within Dshi_3011 at 3180.946 kb on - strand, within Dshi_3011 at 3180.961 kb on - strand, within Dshi_3011 at 3181.033 kb on - strand, within Dshi_3011 at 3181.141 kb on + strand, within Dshi_3011 at 3181.188 kb on - strand, within Dshi_3011 at 3181.188 kb on - strand, within Dshi_3011 at 3181.205 kb on - strand, within Dshi_3011 at 3181.205 kb on - strand, within Dshi_3011 at 3181.252 kb on + strand, within Dshi_3011 at 3181.261 kb on - strand, within Dshi_3011 at 3181.363 kb on + strand at 3181.381 kb on - strand at 3181.428 kb on - strand at 3181.439 kb on - strand at 3181.455 kb on + strand at 3181.462 kb on + strand at 3181.761 kb on + strand, within Dshi_3012 at 3181.769 kb on - strand, within Dshi_3012 at 3181.823 kb on + strand at 3181.831 kb on - strand, within Dshi_3012 at 3181.886 kb on - strand, within Dshi_3012 at 3181.890 kb on - strand, within Dshi_3012 at 3181.908 kb on + strand, within Dshi_3012 at 3181.916 kb on - strand, within Dshi_3012 at 3181.936 kb on + strand, within Dshi_3012 at 3181.984 kb on + strand, within Dshi_3012 at 3182.059 kb on + strand, within Dshi_3012 at 3182.060 kb on + strand, within Dshi_3012 at 3182.172 kb on - strand, within Dshi_3012 at 3182.175 kb on + strand, within Dshi_3012 at 3182.183 kb on - strand, within Dshi_3012 at 3182.183 kb on - strand, within Dshi_3012 at 3182.197 kb on + strand, within Dshi_3012 at 3182.203 kb on + strand, within Dshi_3012 at 3182.211 kb on - strand, within Dshi_3012 at 3182.211 kb on - strand, within Dshi_3012 at 3182.211 kb on - strand, within Dshi_3012 at 3182.231 kb on + strand at 3182.241 kb on + strand at 3182.255 kb on + strand at 3182.271 kb on + strand at 3182.383 kb on - strand at 3182.398 kb on + strand at 3182.504 kb on + strand at 3182.525 kb on + strand, within Dshi_3013 at 3182.547 kb on - strand, within Dshi_3013 at 3182.562 kb on + strand, within Dshi_3013 at 3182.685 kb on - strand, within Dshi_3013 at 3182.686 kb on + strand, within Dshi_3013 at 3182.714 kb on + strand, within Dshi_3013 at 3182.749 kb on + strand, within Dshi_3013 at 3182.767 kb on + strand, within Dshi_3013 at 3182.781 kb on + strand, within Dshi_3013 at 3182.855 kb on - strand, within Dshi_3013 at 3182.916 kb on - strand at 3182.946 kb on - strand at 3183.051 kb on - strand, within Dshi_3014 at 3183.053 kb on + strand, within Dshi_3014 at 3183.084 kb on - strand, within Dshi_3014 at 3183.092 kb on - strand, within Dshi_3014 at 3183.119 kb on + strand, within Dshi_3014 at 3183.192 kb on - strand, within Dshi_3014 at 3183.245 kb on + strand, within Dshi_3014 at 3183.263 kb on + strand, within Dshi_3014 at 3183.285 kb on - strand, within Dshi_3014
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 3,180,629 + Dshi_3011 0.50 -1.5 3,180,799 - Dshi_3011 0.60 -0.4 3,180,801 + Dshi_3011 0.60 +0.3 3,180,809 - Dshi_3011 0.61 +0.0 3,180,913 + Dshi_3011 0.67 -0.1 3,180,919 + Dshi_3011 0.67 -0.5 3,180,946 - Dshi_3011 0.69 -0.5 3,180,946 - Dshi_3011 0.69 +0.0 3,180,946 - Dshi_3011 0.69 +0.2 3,180,961 - Dshi_3011 0.69 -0.5 3,181,033 - Dshi_3011 0.74 -0.3 3,181,141 + Dshi_3011 0.80 -0.6 3,181,188 - Dshi_3011 0.82 +0.1 3,181,188 - Dshi_3011 0.82 -0.5 3,181,205 - Dshi_3011 0.83 -0.4 3,181,205 - Dshi_3011 0.83 +0.8 3,181,252 + Dshi_3011 0.86 +0.1 3,181,261 - Dshi_3011 0.87 -1.2 3,181,363 + +0.1 3,181,381 - -0.5 3,181,428 - +0.7 3,181,439 - -0.3 3,181,455 + -0.3 3,181,462 + +0.8 3,181,761 + Dshi_3012 0.22 -0.8 3,181,769 - Dshi_3012 0.23 -0.5 3,181,823 + -0.6 3,181,831 - Dshi_3012 0.32 -1.1 3,181,886 - Dshi_3012 0.40 +0.4 3,181,890 - Dshi_3012 0.41 +2.0 3,181,908 + Dshi_3012 0.43 +1.0 3,181,916 - Dshi_3012 0.44 +0.3 3,181,936 + Dshi_3012 0.47 +1.0 3,181,984 + Dshi_3012 0.54 +0.5 3,182,059 + Dshi_3012 0.65 +0.2 3,182,060 + Dshi_3012 0.65 +0.2 3,182,172 - Dshi_3012 0.82 +0.5 3,182,175 + Dshi_3012 0.82 +0.3 3,182,183 - Dshi_3012 0.83 +1.0 3,182,183 - Dshi_3012 0.83 +0.1 3,182,197 + Dshi_3012 0.85 -0.1 3,182,203 + Dshi_3012 0.86 +0.8 3,182,211 - Dshi_3012 0.87 +0.3 3,182,211 - Dshi_3012 0.87 +0.8 3,182,211 - Dshi_3012 0.87 +0.1 3,182,231 + +0.5 3,182,241 + +0.2 3,182,255 + -0.6 3,182,271 + +0.4 3,182,383 - +0.2 3,182,398 + -1.0 3,182,504 + -0.1 3,182,525 + Dshi_3013 0.12 +0.4 3,182,547 - Dshi_3013 0.17 +1.0 3,182,562 + Dshi_3013 0.20 -1.2 3,182,685 - Dshi_3013 0.48 +0.4 3,182,686 + Dshi_3013 0.48 -0.3 3,182,714 + Dshi_3013 0.54 -0.3 3,182,749 + Dshi_3013 0.62 -1.2 3,182,767 + Dshi_3013 0.66 +0.0 3,182,781 + Dshi_3013 0.70 -0.1 3,182,855 - Dshi_3013 0.86 +0.3 3,182,916 - -0.5 3,182,946 - -0.6 3,183,051 - Dshi_3014 0.15 -0.1 3,183,053 + Dshi_3014 0.16 -0.1 3,183,084 - Dshi_3014 0.19 +0.8 3,183,092 - Dshi_3014 0.20 -1.3 3,183,119 + Dshi_3014 0.24 +0.3 3,183,192 - Dshi_3014 0.32 -0.9 3,183,245 + Dshi_3014 0.39 +0.0 3,183,263 + Dshi_3014 0.41 +0.7 3,183,285 - Dshi_3014 0.44 -0.4
Or see this region's nucleotide sequence