Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0215 and Dshi_0216 are separated by 66 nucleotides Dshi_0216 and Dshi_0217 overlap by 1 nucleotides Dshi_0217 and Dshi_0218 are separated by 227 nucleotides Dshi_0218 and Dshi_0219 are separated by 3 nucleotides
Dshi_0215: Dshi_0215 - hypothetical protein (RefSeq), at 213,167 to 214,024
_0215
Dshi_0216: Dshi_0216 - Electron transfer flavoprotein alpha subunit (RefSeq), at 214,091 to 215,017
_0216
Dshi_0217: Dshi_0217 - Electron transfer flavoprotein alpha/beta-subunit (RefSeq), at 215,017 to 215,775
_0217
Dshi_0218: Dshi_0218 - ATP--cobalamin adenosyltransferase (RefSeq), at 216,003 to 216,575
_0218
Dshi_0219: Dshi_0219 - hypothetical protein (RefSeq), at 216,579 to 216,794
_0219
Position (kb) 215
216 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 214.032 kb on - strand at 214.073 kb on - strand at 215.802 kb on + strand at 215.952 kb on - strand at 216.009 kb on - strand at 216.079 kb on - strand, within Dshi_0218 at 216.156 kb on - strand, within Dshi_0218 at 216.359 kb on - strand, within Dshi_0218 at 216.359 kb on - strand, within Dshi_0218 at 216.364 kb on - strand, within Dshi_0218 at 216.564 kb on + strand at 216.564 kb on + strand at 216.579 kb on - strand at 216.579 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 214,032 - -0.6 214,073 - +0.2 215,802 + -0.6 215,952 - -1.7 216,009 - +0.0 216,079 - Dshi_0218 0.13 -2.5 216,156 - Dshi_0218 0.27 -3.5 216,359 - Dshi_0218 0.62 -1.6 216,359 - Dshi_0218 0.62 -2.0 216,364 - Dshi_0218 0.63 -1.6 216,564 + -1.0 216,564 + -2.0 216,579 - -0.9 216,579 - -0.2
Or see this region's nucleotide sequence