Experiment: arsenate 0.0005 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1543 and Pf6N2E2_1544 overlap by 11 nucleotides Pf6N2E2_1544 and Pf6N2E2_1545 are separated by 199 nucleotides
Pf6N2E2_1543 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,801,238 to 1,802,719
_1543
Pf6N2E2_1544 - Thermostable hemolysin delta-VPH, at 1,802,709 to 1,803,386
_1544
Pf6N2E2_1545 - N-carbamoylputrescine amidase (EC 3.5.1.53), at 1,803,586 to 1,805,337
_1545
Position (kb)
1802
1803
1804 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1802.115 kb on + strand, within Pf6N2E2_1543 at 1802.115 kb on + strand, within Pf6N2E2_1543 at 1802.116 kb on - strand, within Pf6N2E2_1543 at 1802.116 kb on - strand, within Pf6N2E2_1543 at 1802.127 kb on + strand, within Pf6N2E2_1543 at 1802.128 kb on - strand, within Pf6N2E2_1543 at 1802.166 kb on - strand, within Pf6N2E2_1543 at 1802.593 kb on - strand at 1802.607 kb on + strand at 1802.986 kb on + strand, within Pf6N2E2_1544 at 1802.986 kb on + strand, within Pf6N2E2_1544 at 1802.986 kb on + strand, within Pf6N2E2_1544 at 1802.987 kb on - strand, within Pf6N2E2_1544 at 1802.987 kb on - strand, within Pf6N2E2_1544 at 1802.987 kb on - strand, within Pf6N2E2_1544 at 1802.987 kb on - strand, within Pf6N2E2_1544 at 1802.987 kb on - strand, within Pf6N2E2_1544 at 1803.121 kb on + strand, within Pf6N2E2_1544 at 1803.121 kb on + strand, within Pf6N2E2_1544 at 1803.121 kb on + strand, within Pf6N2E2_1544 at 1803.422 kb on + strand at 1803.423 kb on - strand at 1803.423 kb on - strand at 1803.439 kb on - strand at 1803.523 kb on + strand at 1803.523 kb on + strand at 1803.718 kb on + strand at 1803.872 kb on - strand, within Pf6N2E2_1545 at 1803.889 kb on + strand, within Pf6N2E2_1545 at 1803.889 kb on + strand, within Pf6N2E2_1545 at 1803.889 kb on + strand, within Pf6N2E2_1545 at 1803.890 kb on - strand, within Pf6N2E2_1545 at 1804.087 kb on + strand, within Pf6N2E2_1545 at 1804.228 kb on - strand, within Pf6N2E2_1545
Per-strain Table
Position Strand Gene LocusTag Fraction arsenate 0.0005 M remove 1,802,115 + Pf6N2E2_1543 0.59 -0.0 1,802,115 + Pf6N2E2_1543 0.59 +1.0 1,802,116 - Pf6N2E2_1543 0.59 +0.7 1,802,116 - Pf6N2E2_1543 0.59 +0.4 1,802,127 + Pf6N2E2_1543 0.60 -0.2 1,802,128 - Pf6N2E2_1543 0.60 +0.4 1,802,166 - Pf6N2E2_1543 0.63 -0.5 1,802,593 - +0.3 1,802,607 + +0.0 1,802,986 + Pf6N2E2_1544 0.41 -0.4 1,802,986 + Pf6N2E2_1544 0.41 -0.9 1,802,986 + Pf6N2E2_1544 0.41 -2.5 1,802,987 - Pf6N2E2_1544 0.41 +0.0 1,802,987 - Pf6N2E2_1544 0.41 -0.5 1,802,987 - Pf6N2E2_1544 0.41 -2.2 1,802,987 - Pf6N2E2_1544 0.41 -0.4 1,802,987 - Pf6N2E2_1544 0.41 -0.8 1,803,121 + Pf6N2E2_1544 0.61 -1.9 1,803,121 + Pf6N2E2_1544 0.61 +0.0 1,803,121 + Pf6N2E2_1544 0.61 -1.8 1,803,422 + +1.9 1,803,423 - +1.6 1,803,423 - -0.3 1,803,439 - +0.3 1,803,523 + -0.6 1,803,523 + +1.8 1,803,718 + +1.2 1,803,872 - Pf6N2E2_1545 0.16 +0.8 1,803,889 + Pf6N2E2_1545 0.17 -0.8 1,803,889 + Pf6N2E2_1545 0.17 +0.6 1,803,889 + Pf6N2E2_1545 0.17 +0.1 1,803,890 - Pf6N2E2_1545 0.17 -0.0 1,804,087 + Pf6N2E2_1545 0.29 -1.1 1,804,228 - Pf6N2E2_1545 0.37 -0.3
Or see this region's nucleotide sequence