Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08065 and MMP_RS08070 are separated by 86 nucleotides MMP_RS08070 and MMP_RS08075 overlap by 23 nucleotides MMP_RS08075 and MMP_RS08080 are separated by 10 nucleotides MMP_RS08080 and MMP_RS08085 are separated by 8 nucleotides
MMP_RS08065: MMP_RS08065 - tetrahydromethanopterin S-methyltransferase subunit H, at 1,519,462 to 1,520,421
_RS08065
MMP_RS08070: MMP_RS08070 - DUF2096 domain-containing protein, at 1,520,508 to 1,521,041
_RS08070
MMP_RS08075: MMP_RS08075 - DUF749 domain-containing protein, at 1,521,019 to 1,521,327
_RS08075
MMP_RS08080: MMP_RS08080 - YchF/TatD family DNA exonuclease, at 1,521,338 to 1,522,093
_RS08080
MMP_RS08085: MMP_RS08085 - hypothetical protein, at 1,522,102 to 1,523,037
_RS08085
Position (kb)
1521
1522
1523 Strain fitness (log2 ratio)
-2
-1
0
1 at 1520.424 kb on + strand at 1520.488 kb on - strand at 1520.488 kb on - strand at 1520.488 kb on - strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.510 kb on + strand at 1520.559 kb on - strand at 1520.639 kb on - strand, within MMP_RS08070 at 1520.670 kb on - strand, within MMP_RS08070 at 1520.727 kb on + strand, within MMP_RS08070 at 1520.838 kb on - strand, within MMP_RS08070 at 1520.909 kb on + strand, within MMP_RS08070 at 1520.912 kb on + strand, within MMP_RS08070 at 1520.915 kb on - strand, within MMP_RS08070 at 1521.003 kb on - strand at 1521.004 kb on + strand at 1521.004 kb on + strand at 1521.151 kb on - strand, within MMP_RS08075 at 1521.152 kb on + strand, within MMP_RS08075 at 1521.158 kb on - strand, within MMP_RS08075 at 1521.186 kb on - strand, within MMP_RS08075 at 1521.190 kb on + strand, within MMP_RS08075 at 1521.348 kb on - strand at 1521.350 kb on - strand at 1521.369 kb on - strand at 1521.370 kb on + strand at 1521.370 kb on + strand at 1521.370 kb on + strand at 1521.373 kb on - strand at 1521.493 kb on - strand, within MMP_RS08080 at 1521.493 kb on - strand, within MMP_RS08080 at 1521.564 kb on + strand, within MMP_RS08080 at 1521.572 kb on + strand, within MMP_RS08080 at 1521.577 kb on + strand, within MMP_RS08080 at 1521.755 kb on - strand, within MMP_RS08080 at 1521.849 kb on - strand, within MMP_RS08080 at 1521.868 kb on - strand, within MMP_RS08080 at 1521.881 kb on + strand, within MMP_RS08080 at 1521.909 kb on + strand, within MMP_RS08080 at 1521.977 kb on - strand, within MMP_RS08080 at 1522.010 kb on - strand, within MMP_RS08080 at 1522.015 kb on - strand, within MMP_RS08080 at 1522.015 kb on - strand, within MMP_RS08080 at 1522.071 kb on - strand at 1522.076 kb on + strand at 1522.086 kb on + strand at 1522.123 kb on - strand at 1522.265 kb on + strand, within MMP_RS08085 at 1522.309 kb on - strand, within MMP_RS08085 at 1522.349 kb on + strand, within MMP_RS08085 at 1522.377 kb on - strand, within MMP_RS08085 at 1522.387 kb on - strand, within MMP_RS08085 at 1522.414 kb on - strand, within MMP_RS08085 at 1522.458 kb on + strand, within MMP_RS08085 at 1522.479 kb on - strand, within MMP_RS08085 at 1522.480 kb on + strand, within MMP_RS08085 at 1522.570 kb on + strand, within MMP_RS08085 at 1522.595 kb on + strand, within MMP_RS08085 at 1522.658 kb on - strand, within MMP_RS08085 at 1522.664 kb on + strand, within MMP_RS08085 at 1522.672 kb on + strand, within MMP_RS08085 at 1522.672 kb on + strand, within MMP_RS08085 at 1522.704 kb on + strand, within MMP_RS08085 at 1522.704 kb on + strand, within MMP_RS08085 at 1522.707 kb on + strand, within MMP_RS08085 at 1522.727 kb on + strand, within MMP_RS08085 at 1522.847 kb on + strand, within MMP_RS08085 at 1522.859 kb on + strand, within MMP_RS08085 at 1522.951 kb on + strand at 1522.952 kb on - strand at 1522.991 kb on - strand at 1523.023 kb on - strand at 1523.024 kb on + strand at 1523.029 kb on + strand at 1523.029 kb on + strand at 1523.029 kb on + strand at 1523.029 kb on + strand at 1523.040 kb on - strand at 1523.064 kb on - strand at 1523.088 kb on + strand at 1523.091 kb on - strand at 1523.092 kb on + strand at 1523.092 kb on + strand at 1523.092 kb on + strand at 1523.093 kb on - strand at 1523.093 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,520,424 + -0.4 1,520,488 - -0.2 1,520,488 - +0.0 1,520,488 - +0.4 1,520,489 + -0.4 1,520,489 + +0.0 1,520,489 + -0.1 1,520,489 + +0.1 1,520,489 + +0.3 1,520,489 + +0.1 1,520,489 + +0.6 1,520,510 + +0.5 1,520,559 - -0.5 1,520,639 - MMP_RS08070 0.25 -0.6 1,520,670 - MMP_RS08070 0.30 -0.7 1,520,727 + MMP_RS08070 0.41 -0.5 1,520,838 - MMP_RS08070 0.62 -0.5 1,520,909 + MMP_RS08070 0.75 -0.4 1,520,912 + MMP_RS08070 0.76 -0.0 1,520,915 - MMP_RS08070 0.76 -0.6 1,521,003 - -0.9 1,521,004 + -0.5 1,521,004 + -0.4 1,521,151 - MMP_RS08075 0.43 +0.0 1,521,152 + MMP_RS08075 0.43 -0.4 1,521,158 - MMP_RS08075 0.45 -0.1 1,521,186 - MMP_RS08075 0.54 +0.0 1,521,190 + MMP_RS08075 0.55 -0.5 1,521,348 - -0.1 1,521,350 - -0.4 1,521,369 - -0.2 1,521,370 + -0.1 1,521,370 + -0.3 1,521,370 + -0.4 1,521,373 - +0.3 1,521,493 - MMP_RS08080 0.21 +0.3 1,521,493 - MMP_RS08080 0.21 +0.1 1,521,564 + MMP_RS08080 0.30 -0.5 1,521,572 + MMP_RS08080 0.31 +0.1 1,521,577 + MMP_RS08080 0.32 +0.3 1,521,755 - MMP_RS08080 0.55 +0.2 1,521,849 - MMP_RS08080 0.68 -0.1 1,521,868 - MMP_RS08080 0.70 +0.0 1,521,881 + MMP_RS08080 0.72 -0.2 1,521,909 + MMP_RS08080 0.76 -0.0 1,521,977 - MMP_RS08080 0.85 -0.4 1,522,010 - MMP_RS08080 0.89 -0.4 1,522,015 - MMP_RS08080 0.90 -0.0 1,522,015 - MMP_RS08080 0.90 -0.2 1,522,071 - +0.1 1,522,076 + -0.1 1,522,086 + -0.2 1,522,123 - -1.1 1,522,265 + MMP_RS08085 0.17 -0.2 1,522,309 - MMP_RS08085 0.22 +0.1 1,522,349 + MMP_RS08085 0.26 +0.6 1,522,377 - MMP_RS08085 0.29 +0.2 1,522,387 - MMP_RS08085 0.30 +0.6 1,522,414 - MMP_RS08085 0.33 +1.1 1,522,458 + MMP_RS08085 0.38 -0.0 1,522,479 - MMP_RS08085 0.40 +0.1 1,522,480 + MMP_RS08085 0.40 +0.1 1,522,570 + MMP_RS08085 0.50 +0.3 1,522,595 + MMP_RS08085 0.53 -0.5 1,522,658 - MMP_RS08085 0.59 +0.1 1,522,664 + MMP_RS08085 0.60 +0.2 1,522,672 + MMP_RS08085 0.61 -0.5 1,522,672 + MMP_RS08085 0.61 +0.1 1,522,704 + MMP_RS08085 0.64 -0.3 1,522,704 + MMP_RS08085 0.64 +0.6 1,522,707 + MMP_RS08085 0.65 +0.2 1,522,727 + MMP_RS08085 0.67 -0.5 1,522,847 + MMP_RS08085 0.80 -0.2 1,522,859 + MMP_RS08085 0.81 -0.1 1,522,951 + +0.6 1,522,952 - +0.1 1,522,991 - -0.0 1,523,023 - -0.5 1,523,024 + +0.0 1,523,029 + +0.2 1,523,029 + +1.1 1,523,029 + +0.1 1,523,029 + +1.0 1,523,040 - +0.3 1,523,064 - +0.4 1,523,088 + +0.4 1,523,091 - -2.1 1,523,092 + +0.2 1,523,092 + +0.4 1,523,092 + +0.4 1,523,093 - -0.2 1,523,093 - +0.5
Or see this region's nucleotide sequence