Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03580 and MMP_RS03585 are separated by 163 nucleotides MMP_RS03585 and MMP_RS03590 are separated by 121 nucleotides MMP_RS03590 and MMP_RS03595 are separated by 400 nucleotides
MMP_RS03580: MMP_RS03580 - signal recognition particle-docking protein FtsY, at 671,197 to 672,348
_RS03580
MMP_RS03585: MMP_RS03585 - Hsp20/alpha crystallin family protein, at 672,512 to 672,976
_RS03585
MMP_RS03590: MMP_RS03590 - METTL5 family protein, at 673,098 to 673,739
_RS03590
MMP_RS03595: MMP_RS03595 - 2-amino-3 7-dideoxy-D-threo-hept-6-ulosonate synthase, at 674,140 to 674,958
_RS03595
Position (kb) 673
674 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 672.382 kb on - strand at 672.392 kb on + strand at 672.412 kb on - strand at 672.416 kb on + strand at 672.422 kb on + strand at 672.425 kb on - strand at 672.425 kb on - strand at 672.434 kb on - strand at 672.507 kb on + strand at 672.507 kb on + strand at 672.666 kb on - strand, within MMP_RS03585 at 672.848 kb on - strand, within MMP_RS03585 at 673.000 kb on - strand at 673.028 kb on + strand at 673.050 kb on - strand at 673.052 kb on - strand at 673.096 kb on - strand at 673.274 kb on - strand, within MMP_RS03590 at 673.275 kb on + strand, within MMP_RS03590 at 673.275 kb on + strand, within MMP_RS03590 at 673.277 kb on + strand, within MMP_RS03590 at 673.580 kb on + strand, within MMP_RS03590 at 673.615 kb on + strand, within MMP_RS03590 at 673.648 kb on - strand, within MMP_RS03590 at 673.793 kb on - strand at 673.796 kb on + strand at 673.799 kb on - strand at 673.799 kb on - strand at 673.897 kb on + strand at 673.976 kb on + strand at 674.076 kb on + strand at 674.087 kb on - strand at 674.087 kb on - strand at 674.087 kb on - strand at 674.087 kb on - strand at 674.090 kb on + strand at 674.090 kb on + strand at 674.596 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 672,382 - +0.2 672,392 + -0.7 672,412 - -0.1 672,416 + -0.0 672,422 + -0.0 672,425 - +0.3 672,425 - -0.4 672,434 - +0.1 672,507 + +0.1 672,507 + +0.4 672,666 - MMP_RS03585 0.33 -0.1 672,848 - MMP_RS03585 0.72 +0.3 673,000 - -0.3 673,028 + +0.1 673,050 - +0.4 673,052 - -0.0 673,096 - -0.6 673,274 - MMP_RS03590 0.27 +0.1 673,275 + MMP_RS03590 0.28 -1.1 673,275 + MMP_RS03590 0.28 +0.6 673,277 + MMP_RS03590 0.28 -0.1 673,580 + MMP_RS03590 0.75 +0.1 673,615 + MMP_RS03590 0.81 -0.9 673,648 - MMP_RS03590 0.86 -0.3 673,793 - -1.7 673,796 + -1.4 673,799 - -0.4 673,799 - -2.4 673,897 + -1.3 673,976 + -2.0 674,076 + -1.2 674,087 - -2.7 674,087 - -3.5 674,087 - -0.9 674,087 - -2.1 674,090 + -1.8 674,090 + +0.5 674,596 - -1.2
Or see this region's nucleotide sequence