Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01425 and MMP_RS01430 are separated by 17 nucleotides MMP_RS01430 and MMP_RS01435 are separated by 35 nucleotides MMP_RS01435 and MMP_RS01440 are separated by 39 nucleotides
MMP_RS01425: MMP_RS01425 - tyrosine--tRNA ligase, at 263,898 to 264,827
_RS01425
MMP_RS01430: MMP_RS01430 - mechanosensitive ion channel family protein, at 264,845 to 265,900
_RS01430
MMP_RS01435: MMP_RS01435 - DMT family transporter, at 265,936 to 266,379
_RS01435
MMP_RS01440: MMP_RS01440 - NAAT family transporter, at 266,419 to 267,060
_RS01440
Position (kb)
264
265
266 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 263.902 kb on - strand at 263.909 kb on - strand at 264.233 kb on - strand at 264.238 kb on - strand at 264.238 kb on - strand at 264.291 kb on + strand at 264.341 kb on + strand at 264.368 kb on + strand at 264.512 kb on - strand at 264.547 kb on - strand at 264.547 kb on - strand at 264.942 kb on + strand at 265.180 kb on + strand, within MMP_RS01430 at 265.222 kb on - strand, within MMP_RS01430 at 265.495 kb on - strand, within MMP_RS01430 at 265.501 kb on - strand, within MMP_RS01430 at 265.502 kb on + strand, within MMP_RS01430 at 265.534 kb on - strand, within MMP_RS01430 at 265.535 kb on + strand, within MMP_RS01430 at 265.602 kb on - strand, within MMP_RS01430 at 265.603 kb on + strand, within MMP_RS01430 at 265.648 kb on + strand, within MMP_RS01430 at 265.881 kb on - strand at 265.881 kb on - strand at 265.882 kb on + strand at 265.883 kb on - strand at 265.884 kb on + strand at 265.884 kb on + strand at 266.003 kb on - strand, within MMP_RS01435 at 266.016 kb on + strand, within MMP_RS01435 at 266.141 kb on - strand, within MMP_RS01435 at 266.204 kb on - strand, within MMP_RS01435 at 266.325 kb on - strand, within MMP_RS01435 at 266.346 kb on + strand at 266.500 kb on - strand, within MMP_RS01440
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 263,902 - -2.0 263,909 - -2.3 264,233 - -0.8 264,238 - -2.7 264,238 - -2.7 264,291 + -2.5 264,341 + -0.5 264,368 + -2.5 264,512 - -2.6 264,547 - -0.6 264,547 - -1.5 264,942 + -0.1 265,180 + MMP_RS01430 0.32 -0.4 265,222 - MMP_RS01430 0.36 -0.2 265,495 - MMP_RS01430 0.62 -0.1 265,501 - MMP_RS01430 0.62 -0.5 265,502 + MMP_RS01430 0.62 +0.3 265,534 - MMP_RS01430 0.65 -0.2 265,535 + MMP_RS01430 0.65 -0.3 265,602 - MMP_RS01430 0.72 -0.0 265,603 + MMP_RS01430 0.72 +0.1 265,648 + MMP_RS01430 0.76 -0.1 265,881 - -0.2 265,881 - -0.3 265,882 + +0.0 265,883 - -2.5 265,884 + -3.9 265,884 + -4.8 266,003 - MMP_RS01435 0.15 +0.1 266,016 + MMP_RS01435 0.18 +0.7 266,141 - MMP_RS01435 0.46 +0.2 266,204 - MMP_RS01435 0.60 -0.1 266,325 - MMP_RS01435 0.88 -0.2 266,346 + +0.1 266,500 - MMP_RS01440 0.13 -0.2
Or see this region's nucleotide sequence