Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00340 and MMP_RS00345 are separated by 64 nucleotides MMP_RS00345 and MMP_RS00350 are separated by 108 nucleotides MMP_RS00350 and MMP_RS00355 are separated by 18 nucleotides
MMP_RS00340: MMP_RS00340 - 5 10-methylenetetrahydromethanopterin reductase, at 72,602 to 73,564
_RS00340
MMP_RS00345: MMP_RS00345 - DUF2666 domain-containing protein, at 73,629 to 74,051
_RS00345
MMP_RS00350: MMP_RS00350 - 50S ribosomal protein L18a, at 74,160 to 74,390
_RS00350
MMP_RS00355: MMP_RS00355 - translation initiation factor IF-6, at 74,409 to 75,092
_RS00355
Position (kb)
73
74
75 Strain fitness (log2 ratio)
-2
-1
0
1 at 73.405 kb on + strand at 73.533 kb on - strand at 73.617 kb on + strand at 73.690 kb on + strand, within MMP_RS00345 at 73.821 kb on + strand, within MMP_RS00345 at 73.832 kb on - strand, within MMP_RS00345 at 73.857 kb on - strand, within MMP_RS00345 at 73.917 kb on - strand, within MMP_RS00345 at 74.078 kb on - strand at 74.080 kb on - strand at 74.109 kb on + strand at 74.110 kb on - strand at 74.111 kb on + strand at 74.113 kb on + strand at 74.688 kb on - strand at 74.763 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 73,405 + -1.6 73,533 - -1.9 73,617 + +0.2 73,690 + MMP_RS00345 0.14 -0.4 73,821 + MMP_RS00345 0.45 -0.2 73,832 - MMP_RS00345 0.48 -0.0 73,857 - MMP_RS00345 0.54 -0.4 73,917 - MMP_RS00345 0.68 -1.3 74,078 - +0.3 74,080 - -0.2 74,109 + -0.4 74,110 - -0.3 74,111 + -0.0 74,113 + -0.2 74,688 - -1.8 74,763 - -1.3
Or see this region's nucleotide sequence