Experiment: Survival; Treatment=FW109 (pH 4.4); TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_07270 and IAI47_07275 are separated by 162 nucleotides IAI47_07275 and IAI47_07280 are separated by 16 nucleotides IAI47_07280 and IAI47_07285 are separated by 415 nucleotides IAI47_07285 and IAI47_07290 are separated by 108 nucleotides
IAI47_07270: IAI47_07270 - TetR family transcriptional regulator, at 1,537,642 to 1,538,274
_07270
IAI47_07275: IAI47_07275 - DUF2058 domain-containing protein, at 1,538,437 to 1,538,976
_07275
IAI47_07280: IAI47_07280 - RluA family pseudouridine synthase, at 1,538,993 to 1,539,691
_07280
IAI47_07285: IAI47_07285 - hypothetical protein, at 1,540,107 to 1,540,367
_07285
IAI47_07290: IAI47_07290 - hypothetical protein, at 1,540,476 to 1,540,718
_07290
Position (kb) 1538
1539
1540 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1538.038 kb on - strand, within IAI47_07270 at 1538.059 kb on + strand, within IAI47_07270 at 1538.059 kb on + strand, within IAI47_07270 at 1538.060 kb on - strand, within IAI47_07270 at 1538.060 kb on - strand, within IAI47_07270 at 1538.069 kb on + strand, within IAI47_07270 at 1538.069 kb on + strand, within IAI47_07270 at 1538.070 kb on - strand, within IAI47_07270 at 1538.070 kb on - strand, within IAI47_07270 at 1538.091 kb on + strand, within IAI47_07270 at 1538.091 kb on + strand, within IAI47_07270 at 1538.091 kb on + strand, within IAI47_07270 at 1538.091 kb on + strand, within IAI47_07270 at 1538.091 kb on + strand, within IAI47_07270 at 1538.092 kb on - strand, within IAI47_07270 at 1538.093 kb on + strand, within IAI47_07270 at 1538.093 kb on + strand, within IAI47_07270 at 1538.093 kb on + strand, within IAI47_07270 at 1538.093 kb on + strand, within IAI47_07270 at 1538.093 kb on + strand, within IAI47_07270 at 1538.093 kb on + strand, within IAI47_07270 at 1538.093 kb on + strand, within IAI47_07270 at 1538.093 kb on + strand, within IAI47_07270 at 1538.093 kb on + strand, within IAI47_07270 at 1538.093 kb on + strand, within IAI47_07270 at 1538.094 kb on - strand, within IAI47_07270 at 1538.094 kb on - strand, within IAI47_07270 at 1538.094 kb on - strand, within IAI47_07270 at 1538.094 kb on - strand, within IAI47_07270 at 1538.094 kb on - strand, within IAI47_07270 at 1538.094 kb on - strand, within IAI47_07270 at 1538.094 kb on - strand, within IAI47_07270 at 1538.094 kb on - strand, within IAI47_07270 at 1538.094 kb on - strand, within IAI47_07270 at 1538.206 kb on + strand, within IAI47_07270 at 1538.206 kb on + strand, within IAI47_07270 at 1538.207 kb on - strand, within IAI47_07270 at 1538.207 kb on - strand, within IAI47_07270 at 1538.263 kb on + strand at 1538.264 kb on - strand at 1538.284 kb on + strand at 1538.284 kb on + strand at 1538.284 kb on + strand at 1538.284 kb on + strand at 1538.285 kb on - strand at 1538.285 kb on - strand at 1538.285 kb on - strand at 1538.309 kb on + strand at 1538.387 kb on + strand at 1538.387 kb on + strand at 1538.387 kb on + strand at 1538.388 kb on - strand at 1538.388 kb on - strand at 1538.390 kb on - strand at 1538.400 kb on - strand at 1538.483 kb on + strand at 1538.595 kb on + strand, within IAI47_07275 at 1538.596 kb on - strand, within IAI47_07275 at 1538.642 kb on - strand, within IAI47_07275 at 1538.725 kb on - strand, within IAI47_07275 at 1538.734 kb on - strand, within IAI47_07275 at 1538.734 kb on - strand, within IAI47_07275 at 1538.775 kb on + strand, within IAI47_07275 at 1538.776 kb on - strand, within IAI47_07275 at 1538.776 kb on - strand, within IAI47_07275 at 1538.776 kb on - strand, within IAI47_07275 at 1538.776 kb on - strand, within IAI47_07275 at 1538.831 kb on - strand, within IAI47_07275 at 1538.835 kb on + strand, within IAI47_07275 at 1538.835 kb on + strand, within IAI47_07275 at 1538.836 kb on - strand, within IAI47_07275 at 1538.836 kb on - strand, within IAI47_07275 at 1538.853 kb on - strand, within IAI47_07275 at 1538.906 kb on + strand, within IAI47_07275 at 1538.906 kb on + strand, within IAI47_07275 at 1538.907 kb on - strand, within IAI47_07275 at 1538.938 kb on + strand at 1538.938 kb on + strand at 1538.938 kb on + strand at 1538.938 kb on + strand at 1538.939 kb on - strand at 1538.939 kb on - strand at 1538.939 kb on - strand at 1538.974 kb on + strand at 1538.974 kb on + strand at 1538.975 kb on - strand at 1538.975 kb on - strand at 1538.975 kb on - strand at 1538.975 kb on - strand at 1538.994 kb on + strand at 1538.994 kb on + strand at 1539.023 kb on + strand at 1539.046 kb on + strand at 1539.046 kb on + strand at 1539.046 kb on + strand at 1539.047 kb on - strand at 1539.047 kb on - strand at 1539.047 kb on - strand at 1539.118 kb on - strand, within IAI47_07280 at 1539.164 kb on - strand, within IAI47_07280 at 1539.243 kb on + strand, within IAI47_07280 at 1539.246 kb on + strand, within IAI47_07280 at 1539.247 kb on - strand, within IAI47_07280 at 1539.247 kb on - strand, within IAI47_07280 at 1539.247 kb on - strand, within IAI47_07280 at 1539.302 kb on + strand, within IAI47_07280 at 1539.302 kb on + strand, within IAI47_07280 at 1539.303 kb on - strand, within IAI47_07280 at 1539.396 kb on + strand, within IAI47_07280 at 1539.396 kb on + strand, within IAI47_07280 at 1539.412 kb on + strand, within IAI47_07280 at 1539.412 kb on + strand, within IAI47_07280 at 1539.413 kb on - strand, within IAI47_07280 at 1539.413 kb on - strand, within IAI47_07280 at 1539.445 kb on + strand, within IAI47_07280 at 1539.571 kb on + strand, within IAI47_07280 at 1539.601 kb on + strand, within IAI47_07280 at 1539.601 kb on + strand, within IAI47_07280 at 1539.602 kb on - strand, within IAI47_07280 at 1539.668 kb on - strand at 1539.673 kb on + strand at 1539.673 kb on + strand at 1539.673 kb on + strand at 1539.674 kb on - strand at 1539.679 kb on + strand at 1539.724 kb on + strand at 1539.724 kb on + strand at 1539.724 kb on + strand at 1539.724 kb on + strand at 1539.724 kb on + strand at 1539.724 kb on + strand at 1539.725 kb on - strand at 1539.725 kb on - strand at 1539.725 kb on - strand at 1539.725 kb on - strand at 1539.725 kb on - strand at 1539.725 kb on - strand at 1539.729 kb on + strand at 1539.729 kb on + strand at 1539.729 kb on + strand at 1539.729 kb on + strand at 1539.729 kb on + strand at 1539.730 kb on - strand at 1539.730 kb on - strand at 1539.730 kb on - strand at 1539.730 kb on - strand at 1539.730 kb on - strand at 1539.730 kb on - strand at 1539.773 kb on + strand at 1539.774 kb on - strand at 1539.856 kb on + strand at 1539.856 kb on + strand at 1539.856 kb on + strand at 1539.857 kb on - strand at 1539.866 kb on - strand at 1539.866 kb on - strand at 1539.896 kb on + strand at 1539.926 kb on - strand at 1539.990 kb on - strand at 1540.056 kb on + strand at 1540.056 kb on + strand at 1540.056 kb on + strand at 1540.056 kb on + strand at 1540.057 kb on - strand at 1540.057 kb on - strand at 1540.058 kb on + strand at 1540.058 kb on + strand at 1540.058 kb on + strand at 1540.058 kb on + strand at 1540.059 kb on - strand at 1540.059 kb on - strand at 1540.087 kb on + strand at 1540.112 kb on - strand at 1540.112 kb on - strand at 1540.138 kb on + strand, within IAI47_07285 at 1540.139 kb on - strand, within IAI47_07285 at 1540.139 kb on - strand, within IAI47_07285 at 1540.143 kb on - strand, within IAI47_07285 at 1540.159 kb on + strand, within IAI47_07285 at 1540.159 kb on + strand, within IAI47_07285 at 1540.159 kb on + strand, within IAI47_07285 at 1540.159 kb on + strand, within IAI47_07285 at 1540.160 kb on - strand, within IAI47_07285 at 1540.160 kb on - strand, within IAI47_07285 at 1540.160 kb on - strand, within IAI47_07285 at 1540.190 kb on + strand, within IAI47_07285 at 1540.206 kb on - strand, within IAI47_07285 at 1540.286 kb on + strand, within IAI47_07285 at 1540.286 kb on + strand, within IAI47_07285 at 1540.287 kb on - strand, within IAI47_07285 at 1540.287 kb on - strand, within IAI47_07285 at 1540.292 kb on + strand, within IAI47_07285 at 1540.292 kb on + strand, within IAI47_07285 at 1540.293 kb on - strand, within IAI47_07285 at 1540.293 kb on - strand, within IAI47_07285 at 1540.327 kb on - strand, within IAI47_07285 at 1540.327 kb on - strand, within IAI47_07285 at 1540.366 kb on - strand at 1540.374 kb on + strand at 1540.374 kb on + strand at 1540.395 kb on + strand at 1540.395 kb on + strand at 1540.396 kb on - strand at 1540.396 kb on - strand at 1540.396 kb on - strand at 1540.396 kb on - strand at 1540.454 kb on + strand at 1540.454 kb on + strand at 1540.454 kb on + strand at 1540.454 kb on + strand at 1540.454 kb on + strand at 1540.454 kb on + strand at 1540.455 kb on - strand at 1540.455 kb on - strand at 1540.469 kb on + strand at 1540.469 kb on + strand at 1540.469 kb on + strand at 1540.469 kb on + strand at 1540.470 kb on - strand at 1540.470 kb on - strand at 1540.470 kb on - strand at 1540.470 kb on - strand at 1540.536 kb on + strand, within IAI47_07290 at 1540.539 kb on + strand, within IAI47_07290 at 1540.539 kb on + strand, within IAI47_07290 at 1540.539 kb on + strand, within IAI47_07290 at 1540.540 kb on - strand, within IAI47_07290 at 1540.540 kb on - strand, within IAI47_07290 at 1540.540 kb on - strand, within IAI47_07290 at 1540.540 kb on - strand, within IAI47_07290 at 1540.584 kb on - strand, within IAI47_07290 at 1540.630 kb on + strand, within IAI47_07290 at 1540.630 kb on + strand, within IAI47_07290 at 1540.630 kb on + strand, within IAI47_07290 at 1540.631 kb on - strand, within IAI47_07290 at 1540.631 kb on - strand, within IAI47_07290 at 1540.631 kb on - strand, within IAI47_07290 at 1540.631 kb on - strand, within IAI47_07290 at 1540.644 kb on + strand, within IAI47_07290 at 1540.645 kb on - strand, within IAI47_07290 at 1540.645 kb on - strand, within IAI47_07290 at 1540.684 kb on + strand, within IAI47_07290 at 1540.685 kb on - strand, within IAI47_07290
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW109 (pH 4.4); TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 1,538,038 - IAI47_07270 0.63 +1.5 1,538,059 + IAI47_07270 0.66 -0.1 1,538,059 + IAI47_07270 0.66 +4.5 1,538,060 - IAI47_07270 0.66 -0.0 1,538,060 - IAI47_07270 0.66 +1.2 1,538,069 + IAI47_07270 0.67 -2.3 1,538,069 + IAI47_07270 0.67 -0.4 1,538,070 - IAI47_07270 0.68 +0.7 1,538,070 - IAI47_07270 0.68 -2.3 1,538,091 + IAI47_07270 0.71 +1.3 1,538,091 + IAI47_07270 0.71 +1.3 1,538,091 + IAI47_07270 0.71 -2.7 1,538,091 + IAI47_07270 0.71 -1.0 1,538,091 + IAI47_07270 0.71 -0.3 1,538,092 - IAI47_07270 0.71 +0.4 1,538,093 + IAI47_07270 0.71 +0.1 1,538,093 + IAI47_07270 0.71 +2.0 1,538,093 + IAI47_07270 0.71 -1.3 1,538,093 + IAI47_07270 0.71 +1.4 1,538,093 + IAI47_07270 0.71 -1.7 1,538,093 + IAI47_07270 0.71 -0.7 1,538,093 + IAI47_07270 0.71 -0.7 1,538,093 + IAI47_07270 0.71 -0.1 1,538,093 + IAI47_07270 0.71 +0.8 1,538,093 + IAI47_07270 0.71 +1.8 1,538,094 - IAI47_07270 0.71 -0.7 1,538,094 - IAI47_07270 0.71 +1.4 1,538,094 - IAI47_07270 0.71 -0.7 1,538,094 - IAI47_07270 0.71 +0.9 1,538,094 - IAI47_07270 0.71 -1.9 1,538,094 - IAI47_07270 0.71 +0.1 1,538,094 - IAI47_07270 0.71 +0.5 1,538,094 - IAI47_07270 0.71 +1.0 1,538,094 - IAI47_07270 0.71 -0.0 1,538,206 + IAI47_07270 0.89 +0.4 1,538,206 + IAI47_07270 0.89 +1.1 1,538,207 - IAI47_07270 0.89 -0.6 1,538,207 - IAI47_07270 0.89 +1.4 1,538,263 + -0.1 1,538,264 - +1.0 1,538,284 + +0.7 1,538,284 + +1.1 1,538,284 + -0.5 1,538,284 + +1.1 1,538,285 - +0.9 1,538,285 - +0.7 1,538,285 - +0.5 1,538,309 + -0.1 1,538,387 + +3.5 1,538,387 + +1.0 1,538,387 + +0.0 1,538,388 - -2.2 1,538,388 - +0.1 1,538,390 - -1.7 1,538,400 - -1.6 1,538,483 + -1.9 1,538,595 + IAI47_07275 0.29 -0.1 1,538,596 - IAI47_07275 0.29 +1.5 1,538,642 - IAI47_07275 0.38 -0.8 1,538,725 - IAI47_07275 0.53 -2.2 1,538,734 - IAI47_07275 0.55 -0.3 1,538,734 - IAI47_07275 0.55 -2.3 1,538,775 + IAI47_07275 0.63 -0.7 1,538,776 - IAI47_07275 0.63 -2.3 1,538,776 - IAI47_07275 0.63 -1.1 1,538,776 - IAI47_07275 0.63 -2.5 1,538,776 - IAI47_07275 0.63 -0.6 1,538,831 - IAI47_07275 0.73 -1.4 1,538,835 + IAI47_07275 0.74 -2.8 1,538,835 + IAI47_07275 0.74 -0.3 1,538,836 - IAI47_07275 0.74 -1.3 1,538,836 - IAI47_07275 0.74 -0.3 1,538,853 - IAI47_07275 0.77 -3.4 1,538,906 + IAI47_07275 0.87 -0.4 1,538,906 + IAI47_07275 0.87 -1.1 1,538,907 - IAI47_07275 0.87 +1.4 1,538,938 + -1.3 1,538,938 + +0.1 1,538,938 + +0.3 1,538,938 + +0.4 1,538,939 - +2.1 1,538,939 - +1.7 1,538,939 - -0.6 1,538,974 + +0.2 1,538,974 + +1.7 1,538,975 - +1.2 1,538,975 - -2.0 1,538,975 - -1.7 1,538,975 - -2.3 1,538,994 + +0.4 1,538,994 + +0.9 1,539,023 + -1.6 1,539,046 + -2.0 1,539,046 + -0.4 1,539,046 + +0.9 1,539,047 - +1.3 1,539,047 - -1.9 1,539,047 - -2.2 1,539,118 - IAI47_07280 0.18 +2.3 1,539,164 - IAI47_07280 0.24 +2.7 1,539,243 + IAI47_07280 0.36 +0.1 1,539,246 + IAI47_07280 0.36 -1.1 1,539,247 - IAI47_07280 0.36 +2.6 1,539,247 - IAI47_07280 0.36 +0.7 1,539,247 - IAI47_07280 0.36 -2.0 1,539,302 + IAI47_07280 0.44 +1.2 1,539,302 + IAI47_07280 0.44 +0.9 1,539,303 - IAI47_07280 0.44 -0.6 1,539,396 + IAI47_07280 0.58 +1.2 1,539,396 + IAI47_07280 0.58 +0.9 1,539,412 + IAI47_07280 0.60 -0.4 1,539,412 + IAI47_07280 0.60 +0.9 1,539,413 - IAI47_07280 0.60 -1.6 1,539,413 - IAI47_07280 0.60 -2.3 1,539,445 + IAI47_07280 0.65 +1.7 1,539,571 + IAI47_07280 0.83 +1.7 1,539,601 + IAI47_07280 0.87 +0.7 1,539,601 + IAI47_07280 0.87 -0.9 1,539,602 - IAI47_07280 0.87 -2.0 1,539,668 - +3.0 1,539,673 + +0.1 1,539,673 + +0.8 1,539,673 + +1.3 1,539,674 - +0.1 1,539,679 + -1.9 1,539,724 + -0.3 1,539,724 + +3.3 1,539,724 + +0.1 1,539,724 + -1.4 1,539,724 + +0.1 1,539,724 + -0.6 1,539,725 - -0.1 1,539,725 - +1.8 1,539,725 - +0.1 1,539,725 - -1.6 1,539,725 - -0.1 1,539,725 - +1.4 1,539,729 + -0.3 1,539,729 + +1.1 1,539,729 + -1.6 1,539,729 + -1.3 1,539,729 + +2.5 1,539,730 - -1.9 1,539,730 - +0.2 1,539,730 - +1.8 1,539,730 - +1.0 1,539,730 - +3.7 1,539,730 - -1.2 1,539,773 + +0.7 1,539,774 - +1.2 1,539,856 + +2.1 1,539,856 + -0.9 1,539,856 + +1.9 1,539,857 - -0.9 1,539,866 - -0.1 1,539,866 - +2.1 1,539,896 + -0.4 1,539,926 - -2.0 1,539,990 - -2.1 1,540,056 + +0.1 1,540,056 + -0.7 1,540,056 + +0.0 1,540,056 + +0.9 1,540,057 - -1.6 1,540,057 - -1.4 1,540,058 + +1.5 1,540,058 + -1.4 1,540,058 + +1.7 1,540,058 + -1.2 1,540,059 - +3.0 1,540,059 - +1.1 1,540,087 + +0.9 1,540,112 - -0.1 1,540,112 - -1.1 1,540,138 + IAI47_07285 0.12 +0.9 1,540,139 - IAI47_07285 0.12 +1.9 1,540,139 - IAI47_07285 0.12 +0.0 1,540,143 - IAI47_07285 0.14 -2.6 1,540,159 + IAI47_07285 0.20 +3.4 1,540,159 + IAI47_07285 0.20 -1.7 1,540,159 + IAI47_07285 0.20 +0.1 1,540,159 + IAI47_07285 0.20 +1.4 1,540,160 - IAI47_07285 0.20 -0.8 1,540,160 - IAI47_07285 0.20 -2.3 1,540,160 - IAI47_07285 0.20 -1.6 1,540,190 + IAI47_07285 0.32 -1.9 1,540,206 - IAI47_07285 0.38 -1.4 1,540,286 + IAI47_07285 0.69 -0.3 1,540,286 + IAI47_07285 0.69 +1.3 1,540,287 - IAI47_07285 0.69 -0.4 1,540,287 - IAI47_07285 0.69 -0.3 1,540,292 + IAI47_07285 0.71 -1.3 1,540,292 + IAI47_07285 0.71 -0.1 1,540,293 - IAI47_07285 0.71 +0.9 1,540,293 - IAI47_07285 0.71 -2.1 1,540,327 - IAI47_07285 0.84 +1.1 1,540,327 - IAI47_07285 0.84 -0.9 1,540,366 - +2.8 1,540,374 + -2.5 1,540,374 + +3.0 1,540,395 + -0.7 1,540,395 + +0.2 1,540,396 - -0.5 1,540,396 - -1.4 1,540,396 - -0.5 1,540,396 - -0.1 1,540,454 + +1.3 1,540,454 + -0.4 1,540,454 + +1.3 1,540,454 + +0.1 1,540,454 + -1.1 1,540,454 + -1.4 1,540,455 - -1.1 1,540,455 - -2.6 1,540,469 + -1.1 1,540,469 + -1.1 1,540,469 + -1.6 1,540,469 + -2.1 1,540,470 - +0.8 1,540,470 - -0.4 1,540,470 - +3.0 1,540,470 - -2.3 1,540,536 + IAI47_07290 0.25 -0.9 1,540,539 + IAI47_07290 0.26 +1.7 1,540,539 + IAI47_07290 0.26 -0.4 1,540,539 + IAI47_07290 0.26 +0.6 1,540,540 - IAI47_07290 0.26 +2.2 1,540,540 - IAI47_07290 0.26 +1.9 1,540,540 - IAI47_07290 0.26 -0.4 1,540,540 - IAI47_07290 0.26 -2.8 1,540,584 - IAI47_07290 0.44 +1.7 1,540,630 + IAI47_07290 0.63 +0.5 1,540,630 + IAI47_07290 0.63 +0.4 1,540,630 + IAI47_07290 0.63 +0.3 1,540,631 - IAI47_07290 0.64 -0.9 1,540,631 - IAI47_07290 0.64 +0.4 1,540,631 - IAI47_07290 0.64 -3.2 1,540,631 - IAI47_07290 0.64 +1.4 1,540,644 + IAI47_07290 0.69 +2.7 1,540,645 - IAI47_07290 0.70 -0.7 1,540,645 - IAI47_07290 0.70 -0.5 1,540,684 + IAI47_07290 0.86 +1.5 1,540,685 - IAI47_07290 0.86 -1.4
Or see this region's nucleotide sequence