Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25270

Experiment: Gly-Glu (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25265 and BWI76_RS25270 are separated by 22 nucleotidesBWI76_RS25270 and BWI76_RS25275 are separated by 65 nucleotidesBWI76_RS25275 and BWI76_RS25280 are separated by 45 nucleotides BWI76_RS25265: BWI76_RS25265 - RNA polymerase factor sigma-54, at 5,251,847 to 5,253,280 _RS25265 BWI76_RS25270: BWI76_RS25270 - ribosome hibernation promoting factor, at 5,253,303 to 5,253,590 _RS25270 BWI76_RS25275: BWI76_RS25275 - PTS IIA-like nitrogen regulatory protein PtsN, at 5,253,656 to 5,254,144 _RS25275 BWI76_RS25280: BWI76_RS25280 - RNase adaptor protein RapZ, at 5,254,190 to 5,255,044 _RS25280 Position (kb) 5253 5254Strain fitness (log2 ratio) -2 -1 0 1 2at 5253.303 kb on + strandat 5253.477 kb on + strand, within BWI76_RS25270at 5253.483 kb on + strand, within BWI76_RS25270at 5253.502 kb on + strand, within BWI76_RS25270at 5254.184 kb on + strandat 5254.201 kb on - strandat 5254.555 kb on + strand, within BWI76_RS25280

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Per-strain Table

Position Strand Gene LocusTag Fraction Gly-Glu (C)
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5,253,303 + +2.2
5,253,477 + BWI76_RS25270 0.60 -1.3
5,253,483 + BWI76_RS25270 0.62 +1.8
5,253,502 + BWI76_RS25270 0.69 -1.8
5,254,184 + -0.1
5,254,201 - +1.0
5,254,555 + BWI76_RS25280 0.43 +0.4

Or see this region's nucleotide sequence