Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08845

Experiment: Gly-Glu (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08835 and BWI76_RS08840 are separated by 44 nucleotidesBWI76_RS08840 and BWI76_RS08845 are separated by 190 nucleotidesBWI76_RS08845 and BWI76_RS08850 overlap by 1 nucleotides BWI76_RS08835: BWI76_RS08835 - molybdopterin guanine dinucleotide biosynthesis protein MoaE, at 1,860,767 to 1,861,219 _RS08835 BWI76_RS08840: BWI76_RS08840 - hypothetical protein, at 1,861,264 to 1,861,968 _RS08840 BWI76_RS08845: BWI76_RS08845 - hypothetical protein, at 1,862,159 to 1,863,127 _RS08845 BWI76_RS08850: BWI76_RS08850 - cardiolipin synthase B, at 1,863,127 to 1,864,371 _RS08850 Position (kb) 1862 1863 1864Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6 7 8at 1861.327 kb on + strandat 1861.355 kb on + strand, within BWI76_RS08840at 1861.769 kb on - strand, within BWI76_RS08840at 1862.127 kb on + strandat 1862.269 kb on - strand, within BWI76_RS08845at 1862.541 kb on - strand, within BWI76_RS08845at 1862.678 kb on - strand, within BWI76_RS08845at 1862.710 kb on + strand, within BWI76_RS08845at 1862.710 kb on + strand, within BWI76_RS08845at 1862.756 kb on + strand, within BWI76_RS08845at 1862.764 kb on - strand, within BWI76_RS08845at 1862.839 kb on + strand, within BWI76_RS08845at 1862.898 kb on - strand, within BWI76_RS08845at 1862.910 kb on + strand, within BWI76_RS08845at 1862.967 kb on + strand, within BWI76_RS08845at 1862.967 kb on + strand, within BWI76_RS08845at 1862.980 kb on - strand, within BWI76_RS08845at 1863.314 kb on + strand, within BWI76_RS08850at 1863.349 kb on + strand, within BWI76_RS08850at 1863.499 kb on - strand, within BWI76_RS08850

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Per-strain Table

Position Strand Gene LocusTag Fraction Gly-Glu (C)
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1,861,327 + -1.0
1,861,355 + BWI76_RS08840 0.13 +1.4
1,861,769 - BWI76_RS08840 0.72 -0.3
1,862,127 + +2.4
1,862,269 - BWI76_RS08845 0.11 +0.2
1,862,541 - BWI76_RS08845 0.39 +0.7
1,862,678 - BWI76_RS08845 0.54 -0.2
1,862,710 + BWI76_RS08845 0.57 +8.0
1,862,710 + BWI76_RS08845 0.57 -1.1
1,862,756 + BWI76_RS08845 0.62 -1.1
1,862,764 - BWI76_RS08845 0.62 -0.5
1,862,839 + BWI76_RS08845 0.70 -0.7
1,862,898 - BWI76_RS08845 0.76 +0.2
1,862,910 + BWI76_RS08845 0.78 +1.8
1,862,967 + BWI76_RS08845 0.83 -0.2
1,862,967 + BWI76_RS08845 0.83 -0.4
1,862,980 - BWI76_RS08845 0.85 +0.5
1,863,314 + BWI76_RS08850 0.15 +2.0
1,863,349 + BWI76_RS08850 0.18 +2.5
1,863,499 - BWI76_RS08850 0.30 -0.4

Or see this region's nucleotide sequence