Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdhR and Shew_3433 are separated by 238 nucleotides Shew_3433 and Shew_3434 are separated by 62 nucleotides Shew_3434 and Shew_3435 are separated by 276 nucleotides
Shew_3432: pdhR - transcriptional regulator PdhR (RefSeq), at 4,098,617 to 4,099,369
pdhR
Shew_3433: Shew_3433 - regulatory protein AmpE (RefSeq), at 4,099,608 to 4,100,459
_3433
Shew_3434: Shew_3434 - N-acetyl-anhydromuranmyl-L-alanine amidase (RefSeq), at 4,100,522 to 4,101,076
_3434
Shew_3435: Shew_3435 - nicotinate-nucleotide pyrophosphorylase (RefSeq), at 4,101,353 to 4,102,234
_3435
Position (kb)
4099
4100
4101 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4098.643 kb on + strand at 4098.646 kb on - strand at 4098.953 kb on + strand, within pdhR at 4099.029 kb on + strand, within pdhR at 4099.185 kb on - strand, within pdhR at 4099.515 kb on + strand at 4099.783 kb on - strand, within Shew_3433 at 4099.992 kb on + strand, within Shew_3433 at 4100.001 kb on + strand, within Shew_3433 at 4100.007 kb on + strand, within Shew_3433 at 4100.156 kb on - strand, within Shew_3433 at 4100.162 kb on + strand, within Shew_3433 at 4100.231 kb on + strand, within Shew_3433 at 4100.277 kb on - strand, within Shew_3433 at 4100.510 kb on + strand at 4100.548 kb on + strand at 4100.580 kb on + strand, within Shew_3434 at 4100.598 kb on + strand, within Shew_3434 at 4100.857 kb on + strand, within Shew_3434 at 4100.864 kb on + strand, within Shew_3434 at 4100.864 kb on + strand, within Shew_3434 at 4100.865 kb on - strand, within Shew_3434 at 4100.865 kb on - strand, within Shew_3434 at 4100.865 kb on - strand, within Shew_3434 at 4100.872 kb on - strand, within Shew_3434 at 4100.899 kb on + strand, within Shew_3434 at 4100.928 kb on + strand, within Shew_3434 at 4100.945 kb on - strand, within Shew_3434 at 4100.967 kb on - strand, within Shew_3434 at 4101.081 kb on - strand at 4101.123 kb on - strand at 4101.156 kb on - strand at 4101.209 kb on - strand at 4101.314 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 4,098,643 + +3.9 4,098,646 - +1.6 4,098,953 + pdhR Shew_3432 0.45 +4.0 4,099,029 + pdhR Shew_3432 0.55 +4.5 4,099,185 - pdhR Shew_3432 0.75 +3.2 4,099,515 + +0.2 4,099,783 - Shew_3433 0.21 -0.7 4,099,992 + Shew_3433 0.45 -0.5 4,100,001 + Shew_3433 0.46 -0.2 4,100,007 + Shew_3433 0.47 +0.2 4,100,156 - Shew_3433 0.64 -0.2 4,100,162 + Shew_3433 0.65 -0.4 4,100,231 + Shew_3433 0.73 +0.2 4,100,277 - Shew_3433 0.79 -1.9 4,100,510 + +0.5 4,100,548 + +0.1 4,100,580 + Shew_3434 0.10 +0.5 4,100,598 + Shew_3434 0.14 +0.4 4,100,857 + Shew_3434 0.60 +0.4 4,100,864 + Shew_3434 0.62 +0.8 4,100,864 + Shew_3434 0.62 +0.7 4,100,865 - Shew_3434 0.62 +0.7 4,100,865 - Shew_3434 0.62 +1.0 4,100,865 - Shew_3434 0.62 +0.6 4,100,872 - Shew_3434 0.63 +1.0 4,100,899 + Shew_3434 0.68 +1.4 4,100,928 + Shew_3434 0.73 -0.3 4,100,945 - Shew_3434 0.76 +0.5 4,100,967 - Shew_3434 0.80 +0.8 4,101,081 - -0.3 4,101,123 - +0.1 4,101,156 - +0.1 4,101,209 - -0.0 4,101,314 + +1.6
Or see this region's nucleotide sequence