Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2411 and Shew_2412 are separated by 82 nucleotides Shew_2412 and Shew_2413 are separated by 2 nucleotides Shew_2413 and Shew_2414 are separated by 22 nucleotides Shew_2414 and Shew_2415 are separated by 20 nucleotides
Shew_2411: Shew_2411 - FAD dependent oxidoreductase (RefSeq), at 2,830,891 to 2,832,951
_2411
Shew_2412: Shew_2412 - tRNA/rRNA methyltransferase (SpoU) (RefSeq), at 2,833,034 to 2,833,558
_2412
Shew_2413: Shew_2413 - hypothetical protein (RefSeq), at 2,833,561 to 2,833,842
_2413
Shew_2414: Shew_2414 - ATP-NAD/AcoX kinase (RefSeq), at 2,833,865 to 2,835,031
_2414
Shew_2415: Shew_2415 - major facilitator transporter (RefSeq), at 2,835,052 to 2,836,260
_2415
Position (kb)
2833
2834
2835
2836 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2833.395 kb on + strand, within Shew_2412 at 2833.418 kb on - strand, within Shew_2412 at 2833.802 kb on - strand, within Shew_2413 at 2833.885 kb on + strand at 2834.038 kb on - strand, within Shew_2414 at 2834.640 kb on - strand, within Shew_2414 at 2834.907 kb on + strand, within Shew_2414 at 2835.327 kb on + strand, within Shew_2415
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 2,833,395 + Shew_2412 0.69 -0.1 2,833,418 - Shew_2412 0.73 -1.8 2,833,802 - Shew_2413 0.85 +0.3 2,833,885 + -0.6 2,834,038 - Shew_2414 0.15 +0.8 2,834,640 - Shew_2414 0.66 +0.2 2,834,907 + Shew_2414 0.89 +3.2 2,835,327 + Shew_2415 0.23 +0.2
Or see this region's nucleotide sequence