Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2050 and Shew_2051 are separated by 140 nucleotides Shew_2051 and Shew_2052 are separated by 8 nucleotides Shew_2052 and Shew_2053 are separated by 219 nucleotides
Shew_2050: Shew_2050 - pyruvate kinase (RefSeq), at 2,366,431 to 2,367,870
_2050
Shew_2051: Shew_2051 - acyl-CoA dehydrogenase (RefSeq), at 2,368,011 to 2,370,287
_2051
Shew_2052: Shew_2052 - TetR family transcriptional regulator (RefSeq), at 2,370,296 to 2,370,934
_2052
Shew_2053: Shew_2053 - peptidyl-dipeptidase A (RefSeq), at 2,371,154 to 2,373,058
_2053
Position (kb)
2368
2369
2370
2371 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2367.558 kb on + strand, within Shew_2050 at 2368.106 kb on + strand at 2368.201 kb on + strand at 2368.209 kb on - strand at 2368.209 kb on - strand at 2368.487 kb on - strand, within Shew_2051 at 2369.616 kb on - strand, within Shew_2051 at 2370.059 kb on + strand, within Shew_2051 at 2370.067 kb on - strand at 2370.273 kb on + strand at 2370.418 kb on - strand, within Shew_2052 at 2370.593 kb on + strand, within Shew_2052
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 2,367,558 + Shew_2050 0.78 +0.0 2,368,106 + +0.0 2,368,201 + +0.1 2,368,209 - +0.1 2,368,209 - +3.2 2,368,487 - Shew_2051 0.21 +0.4 2,369,616 - Shew_2051 0.70 +0.5 2,370,059 + Shew_2051 0.90 +3.7 2,370,067 - +0.3 2,370,273 + -0.3 2,370,418 - Shew_2052 0.19 +0.0 2,370,593 + Shew_2052 0.46 +0.1
Or see this region's nucleotide sequence