Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1527 and Shew_1528 are separated by 34 nucleotides Shew_1528 and Shew_1529 are separated by 19 nucleotides Shew_1529 and Shew_R0050 are separated by 159 nucleotides Shew_R0050 and Shew_R0051 are separated by 38 nucleotides Shew_R0051 and Shew_R0052 are separated by 38 nucleotides Shew_R0052 and Shew_R0053 are separated by 174 nucleotides Shew_R0053 and Shew_R0054 are separated by 174 nucleotides
Shew_1527: Shew_1527 - TolB domain-containing protein (RefSeq), at 1,750,081 to 1,751,406
_1527
Shew_1528: Shew_1528 - OmpA domain-containing protein (RefSeq), at 1,751,441 to 1,751,983
_1528
Shew_1529: Shew_1529 - TPR repeat-containing protein (RefSeq), at 1,752,003 to 1,752,725
_1529
Shew_R0050: Shew_R0050 - tRNA-Lys (RefSeq), at 1,752,885 to 1,752,960
_R0050
Shew_R0051: Shew_R0051 - tRNA-Lys (RefSeq), at 1,752,999 to 1,753,074
_R0051
Shew_R0052: Shew_R0052 - tRNA-Lys (RefSeq), at 1,753,113 to 1,753,188
_R0052
Shew_R0053: Shew_R0053 - tRNA-Lys (RefSeq), at 1,753,363 to 1,753,438
_R0053
Shew_R0054: Shew_R0054 - tRNA-Lys (RefSeq), at 1,753,613 to 1,753,688
_R0054
Position (kb)
1752
1753 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1751.140 kb on - strand, within Shew_1527 at 1751.255 kb on + strand, within Shew_1527 at 1751.601 kb on + strand, within Shew_1528 at 1751.984 kb on + strand at 1752.201 kb on - strand, within Shew_1529 at 1752.334 kb on + strand, within Shew_1529 at 1752.334 kb on + strand, within Shew_1529 at 1752.547 kb on - strand, within Shew_1529
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 1,751,140 - Shew_1527 0.80 -2.8 1,751,255 + Shew_1527 0.89 -1.6 1,751,601 + Shew_1528 0.29 -2.2 1,751,984 + -0.9 1,752,201 - Shew_1529 0.27 -1.7 1,752,334 + Shew_1529 0.46 -0.9 1,752,334 + Shew_1529 0.46 -0.6 1,752,547 - Shew_1529 0.75 -1.0
Or see this region's nucleotide sequence