Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1227 and Shew_1228 are separated by 2 nucleotides Shew_1228 and Shew_1229 are separated by 98 nucleotides Shew_1229 and Shew_1230 are separated by 322 nucleotides
Shew_1227: Shew_1227 - glutamate--cysteine ligase (RefSeq), at 1,426,467 to 1,428,038
_1227
Shew_1228: Shew_1228 - hypothetical protein (RefSeq), at 1,428,041 to 1,428,481
_1228
Shew_1229: Shew_1229 - hypothetical protein (RefSeq), at 1,428,580 to 1,429,197
_1229
Shew_1230: Shew_1230 - phosphoribosylaminoimidazole carboxylase, catalytic subunit (RefSeq), at 1,429,520 to 1,430,029
_1230
Position (kb) 1428
1429
1430 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6 at 1427.665 kb on - strand, within Shew_1227 at 1428.879 kb on + strand, within Shew_1229 at 1429.063 kb on + strand, within Shew_1229 at 1429.298 kb on - strand at 1429.538 kb on + strand at 1429.642 kb on + strand, within Shew_1230 at 1429.642 kb on + strand, within Shew_1230 at 1429.845 kb on - strand, within Shew_1230
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 1,427,665 - Shew_1227 0.76 +6.2 1,428,879 + Shew_1229 0.48 +4.9 1,429,063 + Shew_1229 0.78 -0.1 1,429,298 - +0.1 1,429,538 + +2.1 1,429,642 + Shew_1230 0.24 +2.1 1,429,642 + Shew_1230 0.24 +3.5 1,429,845 - Shew_1230 0.64 +2.0
Or see this region's nucleotide sequence