Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1183 and Shew_1184 are separated by 311 nucleotides Shew_1184 and glyA are separated by 342 nucleotides
Shew_1183: Shew_1183 - putative ABC transporter ATP-binding protein (RefSeq), at 1,373,686 to 1,375,350
_1183
Shew_1184: Shew_1184 - hypothetical protein (RefSeq), at 1,375,662 to 1,377,287
_1184
Shew_1185: glyA - serine hydroxymethyltransferase (RefSeq), at 1,377,630 to 1,378,883
glyA
Position (kb)
1375
1376
1377
1378 Strain fitness (log2 ratio)
-1
0
1 at 1375.109 kb on - strand, within Shew_1183 at 1375.697 kb on + strand at 1375.734 kb on + strand at 1375.751 kb on + strand at 1375.751 kb on + strand at 1375.797 kb on + strand at 1375.818 kb on + strand at 1375.864 kb on + strand, within Shew_1184 at 1375.970 kb on - strand, within Shew_1184 at 1376.165 kb on - strand, within Shew_1184 at 1376.204 kb on + strand, within Shew_1184 at 1376.427 kb on + strand, within Shew_1184 at 1376.604 kb on - strand, within Shew_1184 at 1376.718 kb on + strand, within Shew_1184 at 1376.718 kb on + strand, within Shew_1184 at 1376.742 kb on - strand, within Shew_1184 at 1376.896 kb on - strand, within Shew_1184 at 1376.962 kb on + strand, within Shew_1184 at 1376.962 kb on + strand, within Shew_1184 at 1376.970 kb on - strand, within Shew_1184 at 1376.970 kb on - strand, within Shew_1184 at 1377.148 kb on + strand at 1377.689 kb on + strand at 1377.929 kb on + strand, within glyA at 1377.937 kb on - strand, within glyA at 1378.126 kb on + strand, within glyA
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 1,375,109 - Shew_1183 0.85 -1.7 1,375,697 + -0.3 1,375,734 + +0.3 1,375,751 + +0.2 1,375,751 + +0.1 1,375,797 + -0.6 1,375,818 + +0.2 1,375,864 + Shew_1184 0.12 -0.8 1,375,970 - Shew_1184 0.19 +0.2 1,376,165 - Shew_1184 0.31 -0.3 1,376,204 + Shew_1184 0.33 +0.2 1,376,427 + Shew_1184 0.47 -0.2 1,376,604 - Shew_1184 0.58 -0.7 1,376,718 + Shew_1184 0.65 -0.2 1,376,718 + Shew_1184 0.65 +0.1 1,376,742 - Shew_1184 0.66 -0.1 1,376,896 - Shew_1184 0.76 -0.3 1,376,962 + Shew_1184 0.80 -0.6 1,376,962 + Shew_1184 0.80 -0.2 1,376,970 - Shew_1184 0.80 -0.1 1,376,970 - Shew_1184 0.80 -0.2 1,377,148 + +0.0 1,377,689 + +1.6 1,377,929 + glyA Shew_1185 0.24 +1.5 1,377,937 - glyA Shew_1185 0.24 +1.6 1,378,126 + glyA Shew_1185 0.40 +1.7
Or see this region's nucleotide sequence