Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0821 and Shew_0822 are separated by 66 nucleotides Shew_0822 and Shew_0823 are separated by 246 nucleotides Shew_0823 and Shew_0824 are separated by 131 nucleotides Shew_0824 and Shew_0825 are separated by 156 nucleotides
Shew_0821: Shew_0821 - mitomycin resistance protein (RefSeq), at 945,007 to 945,294
_0821
Shew_0822: Shew_0822 - lysine exporter protein LysE/YggA (RefSeq), at 945,361 to 945,954
_0822
Shew_0823: Shew_0823 - LysR family transcriptional regulator (RefSeq), at 946,201 to 947,106
_0823
Shew_0824: Shew_0824 - NAD-dependent epimerase/dehydratase (RefSeq), at 947,238 to 947,879
_0824
Shew_0825: Shew_0825 - ribose-5-phosphate isomerase A (RefSeq), at 948,036 to 948,695
_0825
Position (kb) 946
947
948 Strain fitness (log2 ratio) -1
0
1 at 945.535 kb on + strand, within Shew_0822 at 945.758 kb on + strand, within Shew_0822 at 945.888 kb on + strand, within Shew_0822 at 946.073 kb on + strand at 946.086 kb on + strand at 946.093 kb on - strand at 946.225 kb on - strand at 946.430 kb on + strand, within Shew_0823 at 946.678 kb on - strand, within Shew_0823 at 946.792 kb on + strand, within Shew_0823 at 946.935 kb on + strand, within Shew_0823 at 947.461 kb on + strand, within Shew_0824 at 947.588 kb on - strand, within Shew_0824 at 947.618 kb on + strand, within Shew_0824 at 947.788 kb on + strand, within Shew_0824 at 947.958 kb on + strand at 947.966 kb on - strand at 947.966 kb on - strand at 948.015 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 945,535 + Shew_0822 0.29 +0.6 945,758 + Shew_0822 0.67 -0.1 945,888 + Shew_0822 0.89 +0.7 946,073 + -0.3 946,086 + -0.2 946,093 - +0.2 946,225 - +0.2 946,430 + Shew_0823 0.25 +0.0 946,678 - Shew_0823 0.53 +0.5 946,792 + Shew_0823 0.65 +1.4 946,935 + Shew_0823 0.81 +0.5 947,461 + Shew_0824 0.35 -1.7 947,588 - Shew_0824 0.55 +0.3 947,618 + Shew_0824 0.59 -0.5 947,788 + Shew_0824 0.86 +0.3 947,958 + +0.6 947,966 - +0.2 947,966 - -0.7 948,015 + -1.5
Or see this region's nucleotide sequence