Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0459 and Shew_0460 are separated by 222 nucleotides Shew_0460 and Shew_0461 are separated by 72 nucleotides Shew_0461 and Shew_0462 are separated by 312 nucleotides
Shew_0459: Shew_0459 - PAS/PAC sensor hybrid histidine kinase (RefSeq), at 512,269 to 515,961
_0459
Shew_0460: Shew_0460 - hypothetical protein (RefSeq), at 516,184 to 518,583
_0460
Shew_0461: Shew_0461 - hypothetical protein (RefSeq), at 518,656 to 519,195
_0461
Shew_0462: Shew_0462 - hypothetical protein (RefSeq), at 519,508 to 519,813
_0462
Position (kb) 516
517
518
519 Strain fitness (log2 ratio) -1
0
1 at 515.815 kb on - strand at 516.098 kb on - strand at 516.588 kb on + strand, within Shew_0460 at 516.667 kb on - strand, within Shew_0460 at 516.680 kb on + strand, within Shew_0460 at 516.961 kb on + strand, within Shew_0460 at 517.023 kb on - strand, within Shew_0460 at 517.094 kb on - strand, within Shew_0460 at 517.506 kb on + strand, within Shew_0460 at 517.506 kb on + strand, within Shew_0460 at 517.935 kb on - strand, within Shew_0460 at 518.174 kb on + strand, within Shew_0460 at 518.339 kb on - strand, within Shew_0460 at 518.791 kb on - strand, within Shew_0461 at 518.833 kb on + strand, within Shew_0461 at 519.049 kb on + strand, within Shew_0461 at 519.157 kb on - strand at 519.454 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 515,815 - +0.3 516,098 - -0.9 516,588 + Shew_0460 0.17 -0.1 516,667 - Shew_0460 0.20 -0.6 516,680 + Shew_0460 0.21 -0.2 516,961 + Shew_0460 0.32 -0.1 517,023 - Shew_0460 0.35 -0.3 517,094 - Shew_0460 0.38 -0.3 517,506 + Shew_0460 0.55 -0.9 517,506 + Shew_0460 0.55 +0.1 517,935 - Shew_0460 0.73 -0.1 518,174 + Shew_0460 0.83 -0.1 518,339 - Shew_0460 0.90 +0.0 518,791 - Shew_0461 0.25 +0.0 518,833 + Shew_0461 0.33 +0.2 519,049 + Shew_0461 0.73 -0.3 519,157 - -0.3 519,454 + +0.7
Or see this region's nucleotide sequence