Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0380 and rpmE are separated by 180 nucleotides rpmE and Shew_0382 are separated by 226 nucleotides Shew_0382 and Shew_0383 are separated by 179 nucleotides
Shew_0380: Shew_0380 - hypothetical protein (RefSeq), at 430,339 to 431,115
_0380
Shew_0381: rpmE - 50S ribosomal protein L31 (RefSeq), at 431,296 to 431,508
rpmE
Shew_0382: Shew_0382 - nuclease (RefSeq), at 431,735 to 432,505
_0382
Shew_0383: Shew_0383 - malate dehydrogenase (RefSeq), at 432,685 to 433,932
_0383
Position (kb) 431
432
433 Strain fitness (log2 ratio) -1
0
1
2
3 at 430.797 kb on - strand, within Shew_0380 at 431.181 kb on - strand at 431.625 kb on - strand at 431.625 kb on - strand at 431.644 kb on + strand at 431.765 kb on - strand at 431.928 kb on + strand, within Shew_0382 at 431.928 kb on + strand, within Shew_0382 at 431.928 kb on + strand, within Shew_0382 at 431.928 kb on + strand, within Shew_0382 at 431.928 kb on + strand, within Shew_0382 at 431.928 kb on + strand, within Shew_0382 at 431.936 kb on - strand, within Shew_0382 at 431.936 kb on - strand, within Shew_0382 at 431.936 kb on - strand, within Shew_0382 at 431.936 kb on - strand, within Shew_0382 at 431.936 kb on - strand, within Shew_0382 at 431.936 kb on - strand, within Shew_0382 at 431.936 kb on - strand, within Shew_0382 at 432.098 kb on - strand, within Shew_0382 at 432.583 kb on + strand at 432.591 kb on - strand at 432.792 kb on + strand at 433.246 kb on + strand, within Shew_0383 at 433.254 kb on - strand, within Shew_0383 at 433.498 kb on + strand, within Shew_0383
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 430,797 - Shew_0380 0.59 +0.1 431,181 - -0.1 431,625 - +0.2 431,625 - +0.4 431,644 + -0.6 431,765 - +0.0 431,928 + Shew_0382 0.25 +0.1 431,928 + Shew_0382 0.25 +1.3 431,928 + Shew_0382 0.25 +0.3 431,928 + Shew_0382 0.25 +0.1 431,928 + Shew_0382 0.25 +0.4 431,928 + Shew_0382 0.25 +1.1 431,936 - Shew_0382 0.26 +0.9 431,936 - Shew_0382 0.26 +0.4 431,936 - Shew_0382 0.26 +0.6 431,936 - Shew_0382 0.26 +0.3 431,936 - Shew_0382 0.26 +0.2 431,936 - Shew_0382 0.26 +0.1 431,936 - Shew_0382 0.26 +0.1 432,098 - Shew_0382 0.47 +0.1 432,583 + +3.6 432,591 - +3.3 432,792 + +3.5 433,246 + Shew_0383 0.45 +3.5 433,254 - Shew_0383 0.46 +3.7 433,498 + Shew_0383 0.65 +3.5
Or see this region's nucleotide sequence