Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0334 and sdhB overlap by 4 nucleotides sdhB and Shew_0336 are separated by 191 nucleotides Shew_0336 and rho are separated by 75 nucleotides
Shew_0334: Shew_0334 - fumarate reductase flavoprotein subunit (RefSeq), at 378,664 to 380,670
_0334
Shew_0335: sdhB - fumarate reductase iron-sulfur subunit (RefSeq), at 380,667 to 381,398
sdhB
Shew_0336: Shew_0336 - LysR family transcriptional regulator (RefSeq), at 381,590 to 382,513
_0336
Shew_0337: rho - transcription termination factor Rho (RefSeq), at 382,589 to 383,854
rho
Position (kb) 381
382
383 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 380.607 kb on + strand at 380.678 kb on - strand at 380.915 kb on - strand, within sdhB at 380.999 kb on + strand, within sdhB at 381.010 kb on + strand, within sdhB at 381.155 kb on - strand, within sdhB at 381.750 kb on + strand, within Shew_0336 at 381.758 kb on - strand, within Shew_0336 at 381.758 kb on - strand, within Shew_0336 at 381.758 kb on - strand, within Shew_0336 at 381.760 kb on + strand, within Shew_0336 at 381.870 kb on + strand, within Shew_0336 at 381.908 kb on - strand, within Shew_0336 at 382.034 kb on - strand, within Shew_0336 at 382.106 kb on + strand, within Shew_0336 at 382.217 kb on - strand, within Shew_0336 at 382.221 kb on + strand, within Shew_0336 at 382.221 kb on + strand, within Shew_0336 at 382.425 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 380,607 + -0.2 380,678 - +0.1 380,915 - sdhB Shew_0335 0.34 -0.1 380,999 + sdhB Shew_0335 0.45 +0.1 381,010 + sdhB Shew_0335 0.47 -0.1 381,155 - sdhB Shew_0335 0.67 +0.0 381,750 + Shew_0336 0.17 +0.1 381,758 - Shew_0336 0.18 +0.2 381,758 - Shew_0336 0.18 -0.6 381,758 - Shew_0336 0.18 +5.3 381,760 + Shew_0336 0.18 +1.2 381,870 + Shew_0336 0.30 -0.1 381,908 - Shew_0336 0.34 +0.2 382,034 - Shew_0336 0.48 -0.2 382,106 + Shew_0336 0.56 -0.9 382,217 - Shew_0336 0.68 +0.2 382,221 + Shew_0336 0.68 -0.0 382,221 + Shew_0336 0.68 +0.3 382,425 + +0.3
Or see this region's nucleotide sequence