Experiment: Fusidic 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0131 and Shew_0132 are separated by 107 nucleotides Shew_0132 and Shew_0133 overlap by 10 nucleotides Shew_0133 and Shew_0134 are separated by 47 nucleotides
Shew_0131: Shew_0131 - GCN5-related N-acetyltransferase (RefSeq), at 140,435 to 140,890
_0131
Shew_0132: Shew_0132 - TetR family transcriptional regulator (RefSeq), at 140,998 to 141,654
_0132
Shew_0133: Shew_0133 - phospholipid/glycerol acyltransferase (RefSeq), at 141,645 to 143,507
_0133
Shew_0134: Shew_0134 - tRNA 2-selenouridine synthase (RefSeq), at 143,555 to 144,673
_0134
Position (kb) 141
142
143
144 Strain fitness (log2 ratio) -1
0
1
2
3 at 140.665 kb on + strand, within Shew_0131 at 140.756 kb on - strand, within Shew_0131 at 140.855 kb on + strand at 141.028 kb on - strand at 141.097 kb on - strand, within Shew_0132 at 141.099 kb on + strand, within Shew_0132 at 141.276 kb on - strand, within Shew_0132 at 141.342 kb on + strand, within Shew_0132 at 141.471 kb on + strand, within Shew_0132 at 141.536 kb on + strand, within Shew_0132 at 141.570 kb on + strand, within Shew_0132 at 141.583 kb on - strand, within Shew_0132 at 141.886 kb on + strand, within Shew_0133 at 141.997 kb on + strand, within Shew_0133 at 142.032 kb on - strand, within Shew_0133 at 142.069 kb on + strand, within Shew_0133 at 142.270 kb on - strand, within Shew_0133 at 142.528 kb on + strand, within Shew_0133 at 142.536 kb on - strand, within Shew_0133 at 142.548 kb on + strand, within Shew_0133 at 142.747 kb on - strand, within Shew_0133 at 142.766 kb on - strand, within Shew_0133 at 142.780 kb on - strand, within Shew_0133 at 142.859 kb on + strand, within Shew_0133 at 142.867 kb on - strand, within Shew_0133 at 142.944 kb on - strand, within Shew_0133 at 142.944 kb on - strand, within Shew_0133 at 142.944 kb on - strand, within Shew_0133 at 142.982 kb on - strand, within Shew_0133 at 142.996 kb on + strand, within Shew_0133 at 143.086 kb on - strand, within Shew_0133 at 143.105 kb on + strand, within Shew_0133 at 143.154 kb on - strand, within Shew_0133 at 143.350 kb on - strand at 143.382 kb on - strand at 143.386 kb on + strand at 143.601 kb on - strand at 143.635 kb on - strand at 143.704 kb on + strand, within Shew_0134 at 143.804 kb on - strand, within Shew_0134 at 143.973 kb on + strand, within Shew_0134 at 143.981 kb on - strand, within Shew_0134 at 143.981 kb on - strand, within Shew_0134 at 143.981 kb on - strand, within Shew_0134 at 143.981 kb on - strand, within Shew_0134 at 143.986 kb on - strand, within Shew_0134 at 144.048 kb on + strand, within Shew_0134 at 144.053 kb on + strand, within Shew_0134 at 144.255 kb on - strand, within Shew_0134 at 144.265 kb on + strand, within Shew_0134
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0625 mg/ml remove 140,665 + Shew_0131 0.50 -0.0 140,756 - Shew_0131 0.70 -0.0 140,855 + +0.1 141,028 - +0.4 141,097 - Shew_0132 0.15 -0.0 141,099 + Shew_0132 0.15 +0.4 141,276 - Shew_0132 0.42 -0.2 141,342 + Shew_0132 0.52 +0.6 141,471 + Shew_0132 0.72 +0.5 141,536 + Shew_0132 0.82 -0.0 141,570 + Shew_0132 0.87 +0.5 141,583 - Shew_0132 0.89 +1.7 141,886 + Shew_0133 0.13 +0.3 141,997 + Shew_0133 0.19 -0.9 142,032 - Shew_0133 0.21 +0.2 142,069 + Shew_0133 0.23 -0.4 142,270 - Shew_0133 0.34 +0.1 142,528 + Shew_0133 0.47 -0.3 142,536 - Shew_0133 0.48 -0.5 142,548 + Shew_0133 0.48 -0.4 142,747 - Shew_0133 0.59 -0.1 142,766 - Shew_0133 0.60 +0.1 142,780 - Shew_0133 0.61 +0.3 142,859 + Shew_0133 0.65 +3.3 142,867 - Shew_0133 0.66 -0.3 142,944 - Shew_0133 0.70 +2.2 142,944 - Shew_0133 0.70 +0.4 142,944 - Shew_0133 0.70 +0.1 142,982 - Shew_0133 0.72 +1.3 142,996 + Shew_0133 0.73 +0.6 143,086 - Shew_0133 0.77 +0.3 143,105 + Shew_0133 0.78 -0.6 143,154 - Shew_0133 0.81 -1.5 143,350 - -0.1 143,382 - +0.4 143,386 + -0.0 143,601 - -0.3 143,635 - -0.3 143,704 + Shew_0134 0.13 -0.2 143,804 - Shew_0134 0.22 +0.2 143,973 + Shew_0134 0.37 +0.4 143,981 - Shew_0134 0.38 +1.4 143,981 - Shew_0134 0.38 +0.1 143,981 - Shew_0134 0.38 -0.5 143,981 - Shew_0134 0.38 -0.1 143,986 - Shew_0134 0.39 -0.7 144,048 + Shew_0134 0.44 -0.3 144,053 + Shew_0134 0.45 -0.0 144,255 - Shew_0134 0.63 -0.8 144,265 + Shew_0134 0.63 -0.6
Or see this region's nucleotide sequence