Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03345 and MMP_RS03350 are separated by 39 nucleotides MMP_RS03350 and MMP_RS03355 are separated by 108 nucleotides MMP_RS03355 and MMP_RS03360 are separated by 95 nucleotides MMP_RS03360 and MMP_RS03365 are separated by 19 nucleotides MMP_RS03365 and MMP_RS03370 are separated by 39 nucleotides
MMP_RS03345: MMP_RS03345 - hypothetical protein, at 628,612 to 629,844
_RS03345
MMP_RS03350: MMP_RS03350 - winged helix-turn-helix domain-containing protein, at 629,884 to 630,426
_RS03350
MMP_RS03355: MMP_RS03355 - beta-propeller domain-containing protein, at 630,535 to 632,772
_RS03355
MMP_RS03360: MMP_RS03360 - 50S ribosomal protein L24, at 632,868 to 633,056
_RS03360
MMP_RS03365: MMP_RS03365 - 30S ribosomal protein S28e, at 633,076 to 633,306
_RS03365
MMP_RS03370: MMP_RS03370 - 50S ribosomal protein L7Ae, at 633,346 to 633,699
_RS03370
Position (kb) 630
631
632
633 Strain fitness (log2 ratio) -1
0
1 at 629.557 kb on - strand, within MMP_RS03345 at 629.569 kb on + strand, within MMP_RS03345 at 629.608 kb on - strand, within MMP_RS03345 at 629.674 kb on - strand, within MMP_RS03345 at 629.754 kb on - strand at 629.760 kb on - strand at 629.768 kb on - strand at 629.790 kb on + strand at 629.801 kb on - strand at 629.802 kb on + strand at 629.802 kb on + strand at 629.804 kb on + strand at 629.805 kb on - strand at 629.845 kb on - strand at 629.883 kb on - strand at 629.952 kb on + strand, within MMP_RS03350 at 630.202 kb on + strand, within MMP_RS03350 at 630.203 kb on - strand, within MMP_RS03350 at 630.322 kb on + strand, within MMP_RS03350 at 630.407 kb on - strand at 630.412 kb on - strand at 630.413 kb on + strand at 630.483 kb on - strand at 630.575 kb on - strand at 630.599 kb on + strand at 630.599 kb on + strand at 630.628 kb on - strand at 630.628 kb on - strand at 630.631 kb on + strand at 630.713 kb on + strand at 630.730 kb on + strand at 630.794 kb on + strand, within MMP_RS03355 at 631.032 kb on + strand, within MMP_RS03355 at 631.055 kb on + strand, within MMP_RS03355 at 631.341 kb on - strand, within MMP_RS03355 at 631.352 kb on + strand, within MMP_RS03355 at 631.818 kb on + strand, within MMP_RS03355 at 631.818 kb on + strand, within MMP_RS03355 at 631.879 kb on - strand, within MMP_RS03355 at 631.910 kb on + strand, within MMP_RS03355 at 631.917 kb on + strand, within MMP_RS03355 at 632.019 kb on + strand, within MMP_RS03355 at 632.315 kb on + strand, within MMP_RS03355 at 632.347 kb on - strand, within MMP_RS03355 at 632.348 kb on + strand, within MMP_RS03355 at 632.361 kb on + strand, within MMP_RS03355 at 632.510 kb on - strand, within MMP_RS03355 at 632.582 kb on - strand at 632.589 kb on + strand at 632.589 kb on + strand at 632.619 kb on + strand at 632.739 kb on + strand at 632.739 kb on + strand at 632.742 kb on - strand at 632.837 kb on - strand at 633.691 kb on - strand at 633.692 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 629,557 - MMP_RS03345 0.77 +0.2 629,569 + MMP_RS03345 0.78 -0.4 629,608 - MMP_RS03345 0.81 -0.4 629,674 - MMP_RS03345 0.86 +0.3 629,754 - -0.2 629,760 - +0.7 629,768 - +0.0 629,790 + -0.5 629,801 - -0.4 629,802 + -0.0 629,802 + -0.4 629,804 + -0.3 629,805 - +0.6 629,845 - -0.1 629,883 - -0.1 629,952 + MMP_RS03350 0.13 -0.0 630,202 + MMP_RS03350 0.59 -0.3 630,203 - MMP_RS03350 0.59 +0.7 630,322 + MMP_RS03350 0.81 +0.2 630,407 - -0.2 630,412 - -0.3 630,413 + -0.1 630,483 - +0.2 630,575 - +0.0 630,599 + -0.1 630,599 + +0.0 630,628 - +0.3 630,628 - +0.1 630,631 + +0.4 630,713 + -0.2 630,730 + -0.3 630,794 + MMP_RS03355 0.12 -0.1 631,032 + MMP_RS03355 0.22 -0.2 631,055 + MMP_RS03355 0.23 +0.0 631,341 - MMP_RS03355 0.36 -0.1 631,352 + MMP_RS03355 0.37 +0.5 631,818 + MMP_RS03355 0.57 +0.1 631,818 + MMP_RS03355 0.57 +0.1 631,879 - MMP_RS03355 0.60 -0.1 631,910 + MMP_RS03355 0.61 +0.1 631,917 + MMP_RS03355 0.62 -0.0 632,019 + MMP_RS03355 0.66 -0.1 632,315 + MMP_RS03355 0.80 -0.2 632,347 - MMP_RS03355 0.81 +0.4 632,348 + MMP_RS03355 0.81 +0.6 632,361 + MMP_RS03355 0.82 +0.1 632,510 - MMP_RS03355 0.88 -0.5 632,582 - +0.0 632,589 + +0.1 632,589 + +0.2 632,619 + +0.2 632,739 + +0.2 632,739 + -0.1 632,742 - +0.4 632,837 - +0.1 633,691 - -1.6 633,692 + -1.2
Or see this region's nucleotide sequence