Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01985 and MMP_RS01990 are separated by 203 nucleotides MMP_RS01990 and MMP_RS01995 are separated by 39 nucleotides MMP_RS01995 and MMP_RS02000 are separated by 84 nucleotides
MMP_RS01985: MMP_RS01985 - F420-dependent methylenetetrahydromethanopterin dehydrogenase, at 369,232 to 370,065
_RS01985
MMP_RS01990: MMP_RS01990 - hypothetical protein, at 370,269 to 370,517
_RS01990
MMP_RS01995: MMP_RS01995 - ribonuclease VapC5, at 370,557 to 371,087
_RS01995
MMP_RS02000: MMP_RS02000 - radical SAM protein, at 371,172 to 372,545
_RS02000
Position (kb) 370
371 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 369.361 kb on - strand, within MMP_RS01985 at 369.647 kb on + strand, within MMP_RS01985 at 369.647 kb on + strand, within MMP_RS01985 at 369.683 kb on + strand, within MMP_RS01985 at 369.890 kb on + strand, within MMP_RS01985 at 369.890 kb on + strand, within MMP_RS01985 at 370.067 kb on + strand at 370.067 kb on + strand at 370.067 kb on + strand at 370.100 kb on - strand at 370.120 kb on - strand at 370.121 kb on + strand at 370.178 kb on - strand at 370.202 kb on + strand at 370.202 kb on + strand at 370.215 kb on - strand at 370.223 kb on - strand at 370.318 kb on - strand, within MMP_RS01990 at 370.318 kb on - strand, within MMP_RS01990 at 370.344 kb on + strand, within MMP_RS01990 at 370.382 kb on + strand, within MMP_RS01990 at 370.516 kb on + strand at 370.554 kb on - strand at 370.555 kb on + strand at 370.555 kb on + strand at 370.591 kb on - strand at 370.592 kb on + strand at 370.811 kb on - strand at 371.087 kb on - strand at 371.104 kb on - strand at 371.104 kb on - strand at 371.105 kb on + strand at 371.108 kb on - strand at 371.109 kb on + strand at 371.113 kb on + strand at 371.114 kb on - strand at 371.115 kb on + strand at 371.115 kb on + strand at 371.118 kb on - strand at 371.119 kb on + strand at 371.119 kb on + strand at 371.119 kb on + strand at 371.120 kb on - strand at 371.123 kb on - strand at 371.123 kb on - strand at 371.123 kb on - strand at 371.123 kb on - strand at 371.124 kb on + strand at 371.124 kb on + strand at 371.124 kb on + strand at 371.124 kb on + strand at 371.125 kb on - strand at 371.126 kb on + strand at 371.132 kb on + strand at 371.145 kb on + strand at 371.148 kb on - strand at 371.149 kb on + strand at 371.490 kb on - strand, within MMP_RS02000 at 371.494 kb on + strand, within MMP_RS02000
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 369,361 - MMP_RS01985 0.15 -0.9 369,647 + MMP_RS01985 0.50 -0.9 369,647 + MMP_RS01985 0.50 +0.0 369,683 + MMP_RS01985 0.54 -1.2 369,890 + MMP_RS01985 0.79 -1.0 369,890 + MMP_RS01985 0.79 -1.0 370,067 + -0.8 370,067 + -1.4 370,067 + -1.9 370,100 - -1.0 370,120 - -0.7 370,121 + -0.8 370,178 - +0.6 370,202 + -0.4 370,202 + +0.5 370,215 - -0.2 370,223 - -0.2 370,318 - MMP_RS01990 0.20 -0.1 370,318 - MMP_RS01990 0.20 +0.6 370,344 + MMP_RS01990 0.30 -0.1 370,382 + MMP_RS01990 0.45 -0.2 370,516 + -0.4 370,554 - -0.2 370,555 + -0.2 370,555 + +0.2 370,591 - -0.3 370,592 + -0.3 370,811 - -1.0 371,087 - -3.1 371,104 - -0.1 371,104 - -0.1 371,105 + +0.5 371,108 - -0.3 371,109 + +0.4 371,113 + -0.1 371,114 - -0.2 371,115 + +0.8 371,115 + -0.1 371,118 - +0.2 371,119 + +0.2 371,119 + -0.3 371,119 + -0.2 371,120 - -0.2 371,123 - -0.5 371,123 - +0.2 371,123 - -0.9 371,123 - +0.0 371,124 + -0.7 371,124 + +0.3 371,124 + -0.2 371,124 + -0.1 371,125 - -0.1 371,126 + -0.2 371,132 + +0.6 371,145 + +0.1 371,148 - -0.5 371,149 + -0.1 371,490 - MMP_RS02000 0.23 -0.5 371,494 + MMP_RS02000 0.23 +0.3
Or see this region's nucleotide sequence