Experiment: LB_Anaerobic_60MIN_ZN4B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_09515 and CSW01_09520 are separated by 79 nucleotides CSW01_09520 and CSW01_09525 are separated by 75 nucleotides CSW01_09525 and CSW01_09530 are separated by 177 nucleotides CSW01_09530 and CSW01_09535 are separated by 156 nucleotides
CSW01_09515: CSW01_09515 - Na+/H+ antiporter NhaB, at 2,048,315 to 2,049,907
_09515
CSW01_09520: CSW01_09520 - disulfide bond formation protein B, at 2,049,987 to 2,050,508
_09520
CSW01_09525: CSW01_09525 - DNA translocase FtsK, at 2,050,584 to 2,053,466
_09525
CSW01_09530: CSW01_09530 - leucine-responsive transcriptional regulator Lrp, at 2,053,644 to 2,054,138
_09530
CSW01_09535: CSW01_09535 - alanine dehydrogenase, at 2,054,295 to 2,055,419
_09535
Position (kb)
2050
2051
2052
2053
2054 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2049.584 kb on - strand, within CSW01_09515 at 2049.586 kb on + strand, within CSW01_09515 at 2049.625 kb on + strand, within CSW01_09515 at 2049.637 kb on - strand, within CSW01_09515 at 2049.733 kb on + strand, within CSW01_09515 at 2049.833 kb on - strand at 2049.944 kb on + strand at 2049.977 kb on + strand at 2050.253 kb on - strand, within CSW01_09520 at 2050.288 kb on - strand, within CSW01_09520 at 2050.415 kb on + strand, within CSW01_09520 at 2050.434 kb on + strand, within CSW01_09520 at 2051.085 kb on - strand, within CSW01_09525 at 2051.551 kb on + strand, within CSW01_09525 at 2051.559 kb on - strand, within CSW01_09525 at 2052.053 kb on + strand, within CSW01_09525 at 2052.072 kb on - strand, within CSW01_09525 at 2052.859 kb on + strand, within CSW01_09525 at 2053.312 kb on + strand at 2053.568 kb on + strand at 2053.635 kb on + strand at 2054.248 kb on + strand at 2054.385 kb on + strand at 2054.385 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_Anaerobic_60MIN_ZN4B remove 2,049,584 - CSW01_09515 0.80 +0.2 2,049,586 + CSW01_09515 0.80 -1.7 2,049,625 + CSW01_09515 0.82 -0.0 2,049,637 - CSW01_09515 0.83 -1.0 2,049,733 + CSW01_09515 0.89 -0.2 2,049,833 - +0.4 2,049,944 + +1.2 2,049,977 + +0.4 2,050,253 - CSW01_09520 0.51 -0.8 2,050,288 - CSW01_09520 0.58 +0.1 2,050,415 + CSW01_09520 0.82 -0.4 2,050,434 + CSW01_09520 0.86 -0.2 2,051,085 - CSW01_09525 0.17 +0.3 2,051,551 + CSW01_09525 0.34 -0.7 2,051,559 - CSW01_09525 0.34 -0.7 2,052,053 + CSW01_09525 0.51 +0.3 2,052,072 - CSW01_09525 0.52 -1.3 2,052,859 + CSW01_09525 0.79 +0.3 2,053,312 + +0.3 2,053,568 + +0.6 2,053,635 + -0.2 2,054,248 + -0.1 2,054,385 + -0.0 2,054,385 + -3.2
Or see this region's nucleotide sequence