Experiment: LB_Anaerobic_60MIN_ZN4B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CSW01_00020 and CSW01_00025 are separated by 2 nucleotides CSW01_00025 and CSW01_00030 overlap by 34 nucleotides CSW01_00030 and CSW01_00035 are separated by 12 nucleotides CSW01_00035 and CSW01_00040 are separated by 241 nucleotides
CSW01_00020: CSW01_00020 - membrane protein insertase YidC, at 2,271 to 3,896
_00020
CSW01_00025: CSW01_00025 - membrane protein insertion efficiency factor YidD, at 3,899 to 4,156
_00025
CSW01_00030: CSW01_00030 - ribonuclease P protein component, at 4,123 to 4,479
_00030
CSW01_00035: CSW01_00035 - 50S ribosomal protein L34, at 4,492 to 4,629
_00035
CSW01_00040: CSW01_00040 - amino acid ABC transporter ATP-binding protein, at 4,871 to 5,608
_00040
Position (kb) 3
4
5 Strain fitness (log2 ratio) -2
-1
0
1 at 4.097 kb on + strand, within CSW01_00025 at 4.123 kb on - strand at 4.761 kb on - strand at 4.768 kb on + strand at 4.778 kb on + strand at 4.869 kb on + strand at 4.937 kb on - strand at 4.947 kb on - strand, within CSW01_00040 at 4.949 kb on - strand, within CSW01_00040 at 4.955 kb on - strand, within CSW01_00040 at 5.036 kb on + strand, within CSW01_00040 at 5.069 kb on + strand, within CSW01_00040 at 5.077 kb on - strand, within CSW01_00040 at 5.077 kb on - strand, within CSW01_00040 at 5.079 kb on + strand, within CSW01_00040 at 5.092 kb on - strand, within CSW01_00040
Per-strain Table
Position Strand Gene LocusTag Fraction LB_Anaerobic_60MIN_ZN4B remove 4,097 + CSW01_00025 0.77 -0.9 4,123 - +0.3 4,761 - -1.5 4,768 + +1.0 4,778 + +1.2 4,869 + -0.6 4,937 - -0.7 4,947 - CSW01_00040 0.10 -0.2 4,949 - CSW01_00040 0.11 +0.9 4,955 - CSW01_00040 0.11 +0.4 5,036 + CSW01_00040 0.22 -1.4 5,069 + CSW01_00040 0.27 +0.2 5,077 - CSW01_00040 0.28 -2.6 5,077 - CSW01_00040 0.28 -0.9 5,079 + CSW01_00040 0.28 -0.4 5,092 - CSW01_00040 0.30 -0.6
Or see this region's nucleotide sequence