Experiment: 1-ethyl-3-methylimidazolium chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_02695 and AO356_02700 are separated by 3 nucleotides AO356_02700 and AO356_02705 are separated by 8 nucleotides AO356_02705 and AO356_02710 overlap by 8 nucleotides AO356_02710 and AO356_02715 overlap by 4 nucleotides AO356_02715 and AO356_02720 overlap by 4 nucleotides
AO356_02695: AO356_02695 - type III secretion system protein SpaR, at 591,860 to 592,663
_02695
AO356_02700: AO356_02700 - type III secretion system protein SpaQ, at 592,667 to 592,921
_02700
AO356_02705: AO356_02705 - type III secretion system protein, at 592,930 to 593,589
_02705
AO356_02710: AO356_02710 - flagellar motor switch/type III secretory pathway protein, at 593,582 to 594,511
_02710
AO356_02715: AO356_02715 - type III secretion effector protein, at 594,508 to 595,377
_02715
AO356_02720: AO356_02720 - type III secretion protein, at 595,374 to 595,829
_02720
Position (kb) 593
594
595 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 592.668 kb on + strand at 592.885 kb on - strand, within AO356_02700 at 592.885 kb on - strand, within AO356_02700 at 592.905 kb on + strand at 592.906 kb on - strand at 592.906 kb on - strand at 592.931 kb on + strand at 592.931 kb on + strand at 592.932 kb on - strand at 592.949 kb on + strand at 592.949 kb on + strand at 592.950 kb on - strand at 592.950 kb on - strand at 593.108 kb on + strand, within AO356_02705 at 593.108 kb on + strand, within AO356_02705 at 593.108 kb on + strand, within AO356_02705 at 593.109 kb on - strand, within AO356_02705 at 593.109 kb on - strand, within AO356_02705 at 593.109 kb on - strand, within AO356_02705 at 593.109 kb on - strand, within AO356_02705 at 593.114 kb on + strand, within AO356_02705 at 593.114 kb on + strand, within AO356_02705 at 593.115 kb on - strand, within AO356_02705 at 593.156 kb on + strand, within AO356_02705 at 593.157 kb on - strand, within AO356_02705 at 593.276 kb on + strand, within AO356_02705 at 593.276 kb on + strand, within AO356_02705 at 593.276 kb on + strand, within AO356_02705 at 593.277 kb on - strand, within AO356_02705 at 593.277 kb on - strand, within AO356_02705 at 593.288 kb on + strand, within AO356_02705 at 593.288 kb on + strand, within AO356_02705 at 593.288 kb on + strand, within AO356_02705 at 593.289 kb on - strand, within AO356_02705 at 593.289 kb on - strand, within AO356_02705 at 593.297 kb on + strand, within AO356_02705 at 593.298 kb on - strand, within AO356_02705 at 593.347 kb on - strand, within AO356_02705 at 593.369 kb on + strand, within AO356_02705 at 593.370 kb on - strand, within AO356_02705 at 593.370 kb on - strand, within AO356_02705 at 593.372 kb on + strand, within AO356_02705 at 593.372 kb on + strand, within AO356_02705 at 593.513 kb on + strand, within AO356_02705 at 593.514 kb on - strand, within AO356_02705 at 593.879 kb on + strand, within AO356_02710 at 593.879 kb on + strand, within AO356_02710 at 593.879 kb on + strand, within AO356_02710 at 593.880 kb on - strand, within AO356_02710 at 593.917 kb on - strand, within AO356_02710 at 594.095 kb on + strand, within AO356_02710 at 594.095 kb on + strand, within AO356_02710 at 594.259 kb on + strand, within AO356_02710 at 594.260 kb on - strand, within AO356_02710 at 594.315 kb on + strand, within AO356_02710 at 594.384 kb on + strand, within AO356_02710 at 594.384 kb on + strand, within AO356_02710 at 594.384 kb on + strand, within AO356_02710 at 594.657 kb on + strand, within AO356_02715 at 594.715 kb on + strand, within AO356_02715 at 594.715 kb on + strand, within AO356_02715 at 594.716 kb on - strand, within AO356_02715 at 594.739 kb on - strand, within AO356_02715 at 594.820 kb on + strand, within AO356_02715 at 594.821 kb on - strand, within AO356_02715 at 595.113 kb on + strand, within AO356_02715 at 595.472 kb on - strand, within AO356_02720 at 595.472 kb on - strand, within AO356_02720 at 595.472 kb on - strand, within AO356_02720 at 595.472 kb on - strand, within AO356_02720
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 200 mM remove 592,668 + -1.1 592,885 - AO356_02700 0.85 +1.2 592,885 - AO356_02700 0.85 -0.6 592,905 + -2.3 592,906 - -0.5 592,906 - -0.7 592,931 + -0.4 592,931 + +0.1 592,932 - -1.6 592,949 + +0.1 592,949 + +0.3 592,950 - -0.4 592,950 - +2.3 593,108 + AO356_02705 0.27 -0.9 593,108 + AO356_02705 0.27 +1.7 593,108 + AO356_02705 0.27 +1.3 593,109 - AO356_02705 0.27 -0.5 593,109 - AO356_02705 0.27 -0.7 593,109 - AO356_02705 0.27 +0.1 593,109 - AO356_02705 0.27 -0.4 593,114 + AO356_02705 0.28 +1.2 593,114 + AO356_02705 0.28 +0.4 593,115 - AO356_02705 0.28 -2.4 593,156 + AO356_02705 0.34 -0.4 593,157 - AO356_02705 0.34 -1.2 593,276 + AO356_02705 0.52 +1.5 593,276 + AO356_02705 0.52 -0.1 593,276 + AO356_02705 0.52 -0.3 593,277 - AO356_02705 0.53 +1.2 593,277 - AO356_02705 0.53 -0.0 593,288 + AO356_02705 0.54 -0.3 593,288 + AO356_02705 0.54 +2.1 593,288 + AO356_02705 0.54 +0.5 593,289 - AO356_02705 0.54 +1.1 593,289 - AO356_02705 0.54 -2.0 593,297 + AO356_02705 0.56 -0.9 593,298 - AO356_02705 0.56 -0.7 593,347 - AO356_02705 0.63 +0.2 593,369 + AO356_02705 0.67 -1.3 593,370 - AO356_02705 0.67 -2.3 593,370 - AO356_02705 0.67 +0.2 593,372 + AO356_02705 0.67 +1.4 593,372 + AO356_02705 0.67 -0.0 593,513 + AO356_02705 0.88 +1.0 593,514 - AO356_02705 0.88 -0.1 593,879 + AO356_02710 0.32 +0.7 593,879 + AO356_02710 0.32 -0.1 593,879 + AO356_02710 0.32 +1.3 593,880 - AO356_02710 0.32 -0.5 593,917 - AO356_02710 0.36 +0.5 594,095 + AO356_02710 0.55 +1.6 594,095 + AO356_02710 0.55 -0.1 594,259 + AO356_02710 0.73 -0.5 594,260 - AO356_02710 0.73 +0.1 594,315 + AO356_02710 0.79 +0.5 594,384 + AO356_02710 0.86 +0.2 594,384 + AO356_02710 0.86 -2.5 594,384 + AO356_02710 0.86 +0.5 594,657 + AO356_02715 0.17 -1.2 594,715 + AO356_02715 0.24 +0.4 594,715 + AO356_02715 0.24 -0.4 594,716 - AO356_02715 0.24 -0.5 594,739 - AO356_02715 0.27 -0.3 594,820 + AO356_02715 0.36 -3.8 594,821 - AO356_02715 0.36 +1.4 595,113 + AO356_02715 0.70 +1.3 595,472 - AO356_02720 0.21 -3.0 595,472 - AO356_02720 0.21 -0.3 595,472 - AO356_02720 0.21 -0.4 595,472 - AO356_02720 0.21 -0.6
Or see this region's nucleotide sequence