Experiment: Bacitracin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2254 and Shew_2255 are separated by 48 nucleotides Shew_2255 and trpA overlap by 8 nucleotides
Shew_2254: Shew_2254 - bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (RefSeq), at 2,633,363 to 2,634,814
_2254
Shew_2255: Shew_2255 - tryptophan synthase subunit beta (RefSeq), at 2,634,863 to 2,636,074
_2255
Shew_2256: trpA - tryptophan synthase subunit alpha (RefSeq), at 2,636,067 to 2,636,870
trpA
Position (kb)
2634
2635
2636
2637 Strain fitness (log2 ratio)
-1
0
1 at 2634.032 kb on - strand, within Shew_2254 at 2634.164 kb on - strand, within Shew_2254 at 2634.458 kb on - strand, within Shew_2254 at 2634.537 kb on - strand, within Shew_2254 at 2634.824 kb on + strand at 2634.941 kb on - strand at 2635.100 kb on - strand, within Shew_2255 at 2635.220 kb on + strand, within Shew_2255 at 2635.220 kb on + strand, within Shew_2255 at 2635.228 kb on - strand, within Shew_2255 at 2635.228 kb on - strand, within Shew_2255 at 2635.228 kb on - strand, within Shew_2255 at 2635.241 kb on + strand, within Shew_2255 at 2635.243 kb on - strand, within Shew_2255 at 2635.243 kb on - strand, within Shew_2255 at 2635.263 kb on - strand, within Shew_2255 at 2635.264 kb on - strand, within Shew_2255 at 2635.325 kb on + strand, within Shew_2255 at 2635.758 kb on - strand, within Shew_2255 at 2635.769 kb on + strand, within Shew_2255 at 2635.777 kb on - strand, within Shew_2255 at 2635.909 kb on + strand, within Shew_2255 at 2636.266 kb on + strand, within trpA at 2636.393 kb on - strand, within trpA at 2636.576 kb on + strand, within trpA at 2636.582 kb on + strand, within trpA at 2636.590 kb on - strand, within trpA at 2636.590 kb on - strand, within trpA at 2636.627 kb on + strand, within trpA at 2636.674 kb on - strand, within trpA at 2636.734 kb on - strand, within trpA at 2636.817 kb on + strand at 2636.873 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.125 mg/ml remove 2,634,032 - Shew_2254 0.46 +0.5 2,634,164 - Shew_2254 0.55 +0.4 2,634,458 - Shew_2254 0.75 +0.4 2,634,537 - Shew_2254 0.81 +0.1 2,634,824 + +0.8 2,634,941 - -0.0 2,635,100 - Shew_2255 0.20 +0.2 2,635,220 + Shew_2255 0.29 +0.2 2,635,220 + Shew_2255 0.29 +0.2 2,635,228 - Shew_2255 0.30 -0.1 2,635,228 - Shew_2255 0.30 +0.3 2,635,228 - Shew_2255 0.30 -0.3 2,635,241 + Shew_2255 0.31 +0.3 2,635,243 - Shew_2255 0.31 +0.3 2,635,243 - Shew_2255 0.31 -0.1 2,635,263 - Shew_2255 0.33 +0.1 2,635,264 - Shew_2255 0.33 +1.2 2,635,325 + Shew_2255 0.38 +0.1 2,635,758 - Shew_2255 0.74 +0.4 2,635,769 + Shew_2255 0.75 -0.0 2,635,777 - Shew_2255 0.75 +0.1 2,635,909 + Shew_2255 0.86 -0.3 2,636,266 + trpA Shew_2256 0.25 -0.0 2,636,393 - trpA Shew_2256 0.41 -0.1 2,636,576 + trpA Shew_2256 0.63 +0.2 2,636,582 + trpA Shew_2256 0.64 -0.1 2,636,590 - trpA Shew_2256 0.65 -0.2 2,636,590 - trpA Shew_2256 0.65 +0.1 2,636,627 + trpA Shew_2256 0.70 +0.0 2,636,674 - trpA Shew_2256 0.75 -0.1 2,636,734 - trpA Shew_2256 0.83 +0.0 2,636,817 + +0.0 2,636,873 - -0.3
Or see this region's nucleotide sequence