Experiment: Bacitracin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1446 and Shew_1447 are separated by 263 nucleotides Shew_1447 and Shew_1448 are separated by 108 nucleotides Shew_1448 and Shew_R0034 are separated by 152 nucleotides Shew_R0034 and Shew_R0035 are separated by 38 nucleotides Shew_R0035 and Shew_R0036 are separated by 37 nucleotides Shew_R0036 and Shew_R0037 are separated by 38 nucleotides Shew_R0037 and Shew_R0038 are separated by 59 nucleotides
Shew_1446: Shew_1446 - dicarboxylate TRAP transporter (succinate, fumarate, L-malate, and alpha-ketoglutarate), solute receptor component (from data) , at 1,662,719 to 1,663,729
_1446
Shew_1447: Shew_1447 - major facilitator transporter (RefSeq), at 1,663,993 to 1,665,258
_1447
Shew_1448: Shew_1448 - hypothetical protein (RefSeq), at 1,665,367 to 1,665,594
_1448
Shew_R0034: Shew_R0034 - tRNA-Val (RefSeq), at 1,665,747 to 1,665,822
_R0034
Shew_R0035: Shew_R0035 - tRNA-Val (RefSeq), at 1,665,861 to 1,665,936
_R0035
Shew_R0036: Shew_R0036 - tRNA-Val (RefSeq), at 1,665,974 to 1,666,049
_R0036
Shew_R0037: Shew_R0037 - tRNA-Val (RefSeq), at 1,666,088 to 1,666,163
_R0037
Shew_R0038: Shew_R0038 - tRNA-Val (RefSeq), at 1,666,223 to 1,666,298
_R0038
Position (kb)
1663
1664
1665
1666 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1662.997 kb on - strand, within Shew_1446 at 1663.025 kb on - strand, within Shew_1446 at 1663.037 kb on + strand, within Shew_1446 at 1663.186 kb on + strand, within Shew_1446 at 1663.770 kb on - strand at 1663.916 kb on - strand at 1664.199 kb on - strand, within Shew_1447 at 1664.355 kb on + strand, within Shew_1447 at 1664.493 kb on - strand, within Shew_1447 at 1664.693 kb on + strand, within Shew_1447 at 1664.954 kb on - strand, within Shew_1447 at 1664.966 kb on - strand, within Shew_1447 at 1664.966 kb on + strand, within Shew_1447 at 1665.078 kb on - strand, within Shew_1447 at 1665.113 kb on + strand, within Shew_1447 at 1665.188 kb on - strand at 1665.579 kb on + strand at 1665.690 kb on - strand at 1665.695 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.125 mg/ml remove 1,662,997 - Shew_1446 0.27 -0.2 1,663,025 - Shew_1446 0.30 -0.1 1,663,037 + Shew_1446 0.31 -0.5 1,663,186 + Shew_1446 0.46 +0.2 1,663,770 - +0.1 1,663,916 - +0.0 1,664,199 - Shew_1447 0.16 +0.9 1,664,355 + Shew_1447 0.29 +0.1 1,664,493 - Shew_1447 0.39 -0.6 1,664,693 + Shew_1447 0.55 +0.6 1,664,954 - Shew_1447 0.76 +0.0 1,664,966 - Shew_1447 0.77 +3.9 1,664,966 + Shew_1447 0.77 -0.1 1,665,078 - Shew_1447 0.86 +0.2 1,665,113 + Shew_1447 0.88 +0.2 1,665,188 - -0.3 1,665,579 + +0.1 1,665,690 - +0.1 1,665,695 + +0.2
Or see this region's nucleotide sequence