Experiment: Bacitracin 0.125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1197 and Shew_1198 are separated by 97 nucleotides Shew_1198 and Shew_1199 are separated by 3 nucleotides Shew_1199 and Shew_1200 overlap by 8 nucleotides Shew_1200 and rumA are separated by 97 nucleotides
Shew_1197: Shew_1197 - auxin efflux carrier (RefSeq), at 1,389,101 to 1,390,069
_1197
Shew_1198: Shew_1198 - hypothetical protein (RefSeq), at 1,390,167 to 1,390,361
_1198
Shew_1199: Shew_1199 - hypothetical protein (RefSeq), at 1,390,365 to 1,390,967
_1199
Shew_1200: Shew_1200 - hybrid sensory histidine kinase BarA (RefSeq), at 1,390,960 to 1,393,761
_1200
Shew_1201: rumA - 23S rRNA 5-methyluridine methyltransferase (RefSeq), at 1,393,859 to 1,395,193
rumA
Position (kb)
1390
1391
1392
1393
1394 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1390.127 kb on + strand at 1390.648 kb on + strand, within Shew_1199 at 1390.670 kb on - strand, within Shew_1199 at 1390.979 kb on + strand at 1391.031 kb on + strand at 1391.937 kb on + strand, within Shew_1200 at 1391.937 kb on + strand, within Shew_1200 at 1392.638 kb on - strand, within Shew_1200 at 1392.830 kb on - strand, within Shew_1200 at 1393.205 kb on + strand, within Shew_1200 at 1393.234 kb on - strand, within Shew_1200 at 1393.513 kb on - strand at 1393.816 kb on - strand at 1393.860 kb on - strand at 1393.898 kb on - strand at 1394.062 kb on + strand, within rumA at 1394.229 kb on - strand, within rumA at 1394.535 kb on - strand, within rumA at 1394.642 kb on - strand, within rumA
Per-strain Table
Position Strand Gene LocusTag Fraction Bacitracin 0.125 mg/ml remove 1,390,127 + +0.5 1,390,648 + Shew_1199 0.47 +0.5 1,390,670 - Shew_1199 0.51 +0.0 1,390,979 + +1.3 1,391,031 + -2.1 1,391,937 + Shew_1200 0.35 -3.0 1,391,937 + Shew_1200 0.35 -4.2 1,392,638 - Shew_1200 0.60 -3.0 1,392,830 - Shew_1200 0.67 -2.5 1,393,205 + Shew_1200 0.80 -1.9 1,393,234 - Shew_1200 0.81 -2.5 1,393,513 - -2.3 1,393,816 - -0.6 1,393,860 - -0.6 1,393,898 - +0.6 1,394,062 + rumA Shew_1201 0.15 -0.4 1,394,229 - rumA Shew_1201 0.28 -0.6 1,394,535 - rumA Shew_1201 0.51 -0.6 1,394,642 - rumA Shew_1201 0.59 +0.0
Or see this region's nucleotide sequence