Experiment: aerobic shaking 48 hr copy A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1930 and BT1931 are separated by 877 nucleotides BT1931 and BT1932 overlap by 34 nucleotides BT1932 and BT1933 are separated by 190 nucleotides
BT1930: BT1930 - conserved hypothetical protein (NCBI ptt file), at 2,422,496 to 2,422,789
BT1930
BT1931: BT1931 - putative type IIS restriction/modification enzyme (NCBI ptt file), at 2,423,667 to 2,427,197
BT1931
BT1932: BT1932 - hypothetical protein (NCBI ptt file), at 2,427,164 to 2,427,871
BT1932
BT1933: BT1933 - transposase (NCBI ptt file), at 2,428,062 to 2,428,334
BT1933
Position (kb)
2423
2424
2425
2426
2427
2428 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2422.778 kb on - strand at 2422.896 kb on + strand at 2423.040 kb on + strand at 2423.126 kb on - strand at 2423.159 kb on - strand at 2423.403 kb on - strand at 2423.423 kb on - strand at 2423.494 kb on - strand at 2423.507 kb on - strand at 2423.515 kb on - strand at 2423.516 kb on + strand at 2423.523 kb on + strand at 2423.523 kb on + strand at 2423.523 kb on + strand at 2423.524 kb on - strand at 2423.540 kb on + strand at 2423.600 kb on - strand at 2423.626 kb on + strand at 2423.666 kb on + strand at 2423.674 kb on + strand at 2423.706 kb on + strand at 2423.707 kb on - strand at 2423.778 kb on - strand at 2423.844 kb on + strand at 2423.856 kb on + strand at 2423.856 kb on + strand at 2423.856 kb on + strand at 2423.857 kb on - strand at 2423.907 kb on - strand at 2423.939 kb on - strand at 2423.946 kb on - strand at 2423.973 kb on - strand at 2423.985 kb on - strand at 2424.014 kb on - strand at 2424.015 kb on + strand at 2424.015 kb on + strand at 2424.015 kb on + strand at 2424.015 kb on + strand at 2424.015 kb on + strand at 2424.016 kb on - strand at 2424.018 kb on + strand at 2424.038 kb on + strand, within BT1931 at 2424.039 kb on - strand, within BT1931 at 2424.056 kb on + strand, within BT1931 at 2424.061 kb on - strand, within BT1931 at 2424.074 kb on + strand, within BT1931 at 2424.074 kb on + strand, within BT1931 at 2424.077 kb on + strand, within BT1931 at 2424.142 kb on + strand, within BT1931 at 2424.143 kb on - strand, within BT1931 at 2424.143 kb on - strand, within BT1931 at 2424.246 kb on - strand, within BT1931 at 2424.255 kb on + strand, within BT1931 at 2424.256 kb on - strand, within BT1931 at 2424.262 kb on - strand, within BT1931 at 2424.279 kb on + strand, within BT1931 at 2424.279 kb on + strand, within BT1931 at 2424.296 kb on + strand, within BT1931 at 2424.296 kb on + strand, within BT1931 at 2424.308 kb on + strand, within BT1931 at 2424.338 kb on + strand, within BT1931 at 2424.344 kb on - strand, within BT1931 at 2424.354 kb on - strand, within BT1931 at 2424.425 kb on - strand, within BT1931 at 2424.425 kb on - strand, within BT1931 at 2424.462 kb on - strand, within BT1931 at 2424.515 kb on - strand, within BT1931 at 2424.521 kb on + strand, within BT1931 at 2424.521 kb on - strand, within BT1931 at 2424.562 kb on + strand, within BT1931 at 2424.562 kb on + strand, within BT1931 at 2424.563 kb on - strand, within BT1931 at 2424.563 kb on - strand, within BT1931 at 2424.588 kb on + strand, within BT1931 at 2424.626 kb on + strand, within BT1931 at 2424.629 kb on + strand, within BT1931 at 2424.721 kb on + strand, within BT1931 at 2424.739 kb on - strand, within BT1931 at 2424.739 kb on - strand, within BT1931 at 2424.882 kb on - strand, within BT1931 at 2424.905 kb on + strand, within BT1931 at 2424.906 kb on - strand, within BT1931 at 2424.910 kb on + strand, within BT1931 at 2424.911 kb on - strand, within BT1931 at 2424.911 kb on - strand, within BT1931 at 2424.934 kb on + strand, within BT1931 at 2424.934 kb on + strand, within BT1931 at 2424.935 kb on - strand, within BT1931 at 2424.959 kb on - strand, within BT1931 at 2424.988 kb on - strand, within BT1931 at 2425.012 kb on - strand, within BT1931 at 2425.013 kb on + strand, within BT1931 at 2425.014 kb on - strand, within BT1931 at 2425.090 kb on + strand, within BT1931 at 2425.090 kb on + strand, within BT1931 at 2425.229 kb on + strand, within BT1931 at 2425.240 kb on - strand, within BT1931 at 2425.240 kb on - strand, within BT1931 at 2425.280 kb on + strand, within BT1931 at 2425.479 kb on + strand, within BT1931 at 2425.572 kb on - strand, within BT1931 at 2425.635 kb on + strand, within BT1931 at 2425.638 kb on + strand, within BT1931 at 2425.639 kb on - strand, within BT1931 at 2425.662 kb on + strand, within BT1931 at 2425.662 kb on + strand, within BT1931 at 2425.691 kb on + strand, within BT1931 at 2425.743 kb on - strand, within BT1931 at 2425.743 kb on - strand, within BT1931 at 2425.766 kb on + strand, within BT1931 at 2425.766 kb on + strand, within BT1931 at 2425.770 kb on - strand, within BT1931 at 2425.787 kb on + strand, within BT1931 at 2425.787 kb on + strand, within BT1931 at 2425.788 kb on - strand, within BT1931 at 2425.808 kb on + strand, within BT1931 at 2425.853 kb on + strand, within BT1931 at 2425.948 kb on + strand, within BT1931 at 2425.948 kb on + strand, within BT1931 at 2426.038 kb on + strand, within BT1931 at 2426.046 kb on - strand, within BT1931 at 2426.059 kb on + strand, within BT1931 at 2426.059 kb on + strand, within BT1931 at 2426.116 kb on + strand, within BT1931 at 2426.154 kb on - strand, within BT1931 at 2426.213 kb on - strand, within BT1931 at 2426.213 kb on - strand, within BT1931 at 2426.226 kb on + strand, within BT1931 at 2426.226 kb on + strand, within BT1931 at 2426.227 kb on - strand, within BT1931 at 2426.231 kb on - strand, within BT1931 at 2426.235 kb on + strand, within BT1931 at 2426.235 kb on + strand, within BT1931 at 2426.236 kb on - strand, within BT1931 at 2426.236 kb on - strand, within BT1931 at 2426.269 kb on - strand, within BT1931 at 2426.291 kb on + strand, within BT1931 at 2426.314 kb on - strand, within BT1931 at 2426.315 kb on + strand, within BT1931 at 2426.315 kb on + strand, within BT1931 at 2426.316 kb on - strand, within BT1931 at 2426.441 kb on - strand, within BT1931 at 2426.450 kb on + strand, within BT1931 at 2426.580 kb on - strand, within BT1931 at 2426.593 kb on + strand, within BT1931 at 2426.593 kb on + strand, within BT1931 at 2426.644 kb on + strand at 2426.684 kb on - strand, within BT1931 at 2426.708 kb on + strand, within BT1931 at 2426.737 kb on + strand, within BT1931 at 2426.737 kb on + strand, within BT1931 at 2426.829 kb on + strand, within BT1931 at 2426.852 kb on + strand at 2426.852 kb on + strand at 2426.884 kb on + strand at 2426.884 kb on + strand at 2427.026 kb on + strand at 2427.028 kb on - strand at 2427.102 kb on + strand at 2427.117 kb on + strand at 2427.121 kb on + strand at 2427.138 kb on + strand at 2427.139 kb on - strand at 2427.140 kb on + strand at 2427.192 kb on - strand at 2427.217 kb on + strand at 2427.254 kb on - strand, within BT1932 at 2427.256 kb on - strand, within BT1932 at 2427.265 kb on - strand, within BT1932 at 2427.275 kb on + strand, within BT1932 at 2427.286 kb on - strand, within BT1932 at 2427.286 kb on - strand, within BT1932 at 2427.305 kb on + strand, within BT1932 at 2427.305 kb on + strand, within BT1932 at 2427.350 kb on + strand, within BT1932 at 2427.350 kb on + strand, within BT1932 at 2427.355 kb on + strand, within BT1932 at 2427.355 kb on + strand, within BT1932 at 2427.356 kb on - strand, within BT1932 at 2427.425 kb on - strand, within BT1932 at 2427.770 kb on + strand, within BT1932 at 2427.824 kb on - strand at 2427.968 kb on + strand at 2427.978 kb on - strand at 2427.987 kb on + strand at 2427.988 kb on - strand at 2428.043 kb on + strand at 2428.044 kb on - strand at 2428.044 kb on - strand at 2428.071 kb on + strand at 2428.072 kb on - strand at 2428.073 kb on + strand at 2428.074 kb on - strand at 2428.082 kb on + strand at 2428.142 kb on - strand, within BT1933 at 2428.142 kb on - strand, within BT1933 at 2428.171 kb on + strand, within BT1933 at 2428.172 kb on - strand, within BT1933 at 2428.172 kb on - strand, within BT1933
Per-strain Table
Position Strand Gene LocusTag Fraction aerobic shaking 48 hr copy A remove 2,422,778 - +1.6 2,422,896 + -1.0 2,423,040 + -1.2 2,423,126 - -1.1 2,423,159 - -1.2 2,423,403 - +0.4 2,423,423 - -0.7 2,423,494 - -1.5 2,423,507 - +1.2 2,423,515 - -0.4 2,423,516 + -1.7 2,423,523 + +0.1 2,423,523 + +1.2 2,423,523 + -1.8 2,423,524 - -3.3 2,423,540 + -0.7 2,423,600 - -2.0 2,423,626 + -0.9 2,423,666 + -0.9 2,423,674 + -1.7 2,423,706 + +0.1 2,423,707 - -0.9 2,423,778 - -0.4 2,423,844 + -1.1 2,423,856 + +0.6 2,423,856 + -0.4 2,423,856 + +0.9 2,423,857 - -1.5 2,423,907 - -3.5 2,423,939 - -0.9 2,423,946 - +0.1 2,423,973 - -1.7 2,423,985 - -4.0 2,424,014 - +0.3 2,424,015 + -0.4 2,424,015 + -1.1 2,424,015 + -0.9 2,424,015 + -1.5 2,424,015 + -0.7 2,424,016 - -1.0 2,424,018 + +0.4 2,424,038 + BT1931 0.11 -1.9 2,424,039 - BT1931 0.11 -0.4 2,424,056 + BT1931 0.11 +0.3 2,424,061 - BT1931 0.11 +0.1 2,424,074 + BT1931 0.12 -2.5 2,424,074 + BT1931 0.12 -1.5 2,424,077 + BT1931 0.12 -0.9 2,424,142 + BT1931 0.13 -0.1 2,424,143 - BT1931 0.13 +0.1 2,424,143 - BT1931 0.13 +0.1 2,424,246 - BT1931 0.16 -2.8 2,424,255 + BT1931 0.17 -1.7 2,424,256 - BT1931 0.17 -0.9 2,424,262 - BT1931 0.17 -1.4 2,424,279 + BT1931 0.17 -1.6 2,424,279 + BT1931 0.17 -1.8 2,424,296 + BT1931 0.18 -0.8 2,424,296 + BT1931 0.18 -1.7 2,424,308 + BT1931 0.18 -1.9 2,424,338 + BT1931 0.19 -1.6 2,424,344 - BT1931 0.19 -3.3 2,424,354 - BT1931 0.19 -1.8 2,424,425 - BT1931 0.21 -1.1 2,424,425 - BT1931 0.21 +0.4 2,424,462 - BT1931 0.23 -0.5 2,424,515 - BT1931 0.24 -1.9 2,424,521 + BT1931 0.24 +0.1 2,424,521 - BT1931 0.24 +0.6 2,424,562 + BT1931 0.25 -2.8 2,424,562 + BT1931 0.25 -1.5 2,424,563 - BT1931 0.25 -1.5 2,424,563 - BT1931 0.25 -2.2 2,424,588 + BT1931 0.26 -0.7 2,424,626 + BT1931 0.27 -0.7 2,424,629 + BT1931 0.27 -2.7 2,424,721 + BT1931 0.30 +0.1 2,424,739 - BT1931 0.30 +0.3 2,424,739 - BT1931 0.30 -1.9 2,424,882 - BT1931 0.34 -1.8 2,424,905 + BT1931 0.35 -0.5 2,424,906 - BT1931 0.35 +1.1 2,424,910 + BT1931 0.35 -1.6 2,424,911 - BT1931 0.35 -0.6 2,424,911 - BT1931 0.35 +1.2 2,424,934 + BT1931 0.36 -0.9 2,424,934 + BT1931 0.36 +0.0 2,424,935 - BT1931 0.36 -1.2 2,424,959 - BT1931 0.37 +1.6 2,424,988 - BT1931 0.37 -0.7 2,425,012 - BT1931 0.38 -2.7 2,425,013 + BT1931 0.38 -1.4 2,425,014 - BT1931 0.38 +0.1 2,425,090 + BT1931 0.40 -0.8 2,425,090 + BT1931 0.40 -0.4 2,425,229 + BT1931 0.44 -1.5 2,425,240 - BT1931 0.45 -0.2 2,425,240 - BT1931 0.45 -0.4 2,425,280 + BT1931 0.46 -1.7 2,425,479 + BT1931 0.51 -0.9 2,425,572 - BT1931 0.54 +0.6 2,425,635 + BT1931 0.56 -2.5 2,425,638 + BT1931 0.56 -1.5 2,425,639 - BT1931 0.56 -0.4 2,425,662 + BT1931 0.56 -1.8 2,425,662 + BT1931 0.56 -2.2 2,425,691 + BT1931 0.57 -1.5 2,425,743 - BT1931 0.59 -1.2 2,425,743 - BT1931 0.59 -0.9 2,425,766 + BT1931 0.59 +1.6 2,425,766 + BT1931 0.59 -2.1 2,425,770 - BT1931 0.60 -0.9 2,425,787 + BT1931 0.60 -1.6 2,425,787 + BT1931 0.60 -1.5 2,425,788 - BT1931 0.60 -1.8 2,425,808 + BT1931 0.61 -2.6 2,425,853 + BT1931 0.62 -1.2 2,425,948 + BT1931 0.65 -0.7 2,425,948 + BT1931 0.65 +0.6 2,426,038 + BT1931 0.67 +0.7 2,426,046 - BT1931 0.67 -2.4 2,426,059 + BT1931 0.68 +0.4 2,426,059 + BT1931 0.68 -0.2 2,426,116 + BT1931 0.69 +0.3 2,426,154 - BT1931 0.70 +0.6 2,426,213 - BT1931 0.72 -1.5 2,426,213 - BT1931 0.72 -0.7 2,426,226 + BT1931 0.72 +0.1 2,426,226 + BT1931 0.72 -0.1 2,426,227 - BT1931 0.73 -2.6 2,426,231 - BT1931 0.73 -0.4 2,426,235 + BT1931 0.73 +0.1 2,426,235 + BT1931 0.73 +0.9 2,426,236 - BT1931 0.73 -2.2 2,426,236 - BT1931 0.73 +0.3 2,426,269 - BT1931 0.74 -3.1 2,426,291 + BT1931 0.74 -1.7 2,426,314 - BT1931 0.75 -2.1 2,426,315 + BT1931 0.75 -0.6 2,426,315 + BT1931 0.75 -0.7 2,426,316 - BT1931 0.75 -1.8 2,426,441 - BT1931 0.79 +0.1 2,426,450 + BT1931 0.79 +0.6 2,426,580 - BT1931 0.82 -0.6 2,426,593 + BT1931 0.83 +0.8 2,426,593 + BT1931 0.83 -0.7 2,426,644 + -0.4 2,426,684 - BT1931 0.85 -1.7 2,426,708 + BT1931 0.86 -0.8 2,426,737 + BT1931 0.87 -1.8 2,426,737 + BT1931 0.87 -2.0 2,426,829 + BT1931 0.90 -0.4 2,426,852 + -1.2 2,426,852 + -2.2 2,426,884 + -1.4 2,426,884 + +0.6 2,427,026 + +0.1 2,427,028 - -1.4 2,427,102 + -0.7 2,427,117 + -1.4 2,427,121 + -0.7 2,427,138 + +0.6 2,427,139 - +0.1 2,427,140 + -1.5 2,427,192 - +0.3 2,427,217 + -0.2 2,427,254 - BT1932 0.13 -1.5 2,427,256 - BT1932 0.13 -1.1 2,427,265 - BT1932 0.14 -1.8 2,427,275 + BT1932 0.16 +0.6 2,427,286 - BT1932 0.17 -1.2 2,427,286 - BT1932 0.17 -0.1 2,427,305 + BT1932 0.20 -1.9 2,427,305 + BT1932 0.20 -1.5 2,427,350 + BT1932 0.26 -0.9 2,427,350 + BT1932 0.26 +1.1 2,427,355 + BT1932 0.27 -0.7 2,427,355 + BT1932 0.27 -2.3 2,427,356 - BT1932 0.27 -3.9 2,427,425 - BT1932 0.37 -0.8 2,427,770 + BT1932 0.86 -1.7 2,427,824 - +0.1 2,427,968 + +0.1 2,427,978 - -1.8 2,427,987 + -1.2 2,427,988 - -1.5 2,428,043 + +0.3 2,428,044 - -2.5 2,428,044 - -1.6 2,428,071 + -1.4 2,428,072 - +0.1 2,428,073 + -1.0 2,428,074 - -1.2 2,428,082 + -3.4 2,428,142 - BT1933 0.29 -2.2 2,428,142 - BT1933 0.29 -0.2 2,428,171 + BT1933 0.40 -1.2 2,428,172 - BT1933 0.40 -1.3 2,428,172 - BT1933 0.40 -1.2
Or see this region's nucleotide sequence