Experiment: H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04785 and MMP_RS04790 are separated by 8 nucleotides MMP_RS04790 and MMP_RS04795 are separated by 8 nucleotides MMP_RS04795 and MMP_RS04800 are separated by 10 nucleotides MMP_RS04800 and MMP_RS04805 are separated by 44 nucleotides
MMP_RS04785: MMP_RS04785 - hypothetical protein, at 911,073 to 911,723
_RS04785
MMP_RS04790: MMP_RS04790 - 4-hydroxy-tetrahydrodipicolinate reductase, at 911,732 to 912,544
_RS04790
MMP_RS04795: MMP_RS04795 - tRNA (pseudouridine(54)-N(1))-methyltransferase TrmY, at 912,553 to 913,155
_RS04795
MMP_RS04800: MMP_RS04800 - chemotaxis protein CheW, at 913,166 to 913,609
_RS04800
MMP_RS04805: MMP_RS04805 - chemotaxis response regulator protein-glutamate methylesterase, at 913,654 to 914,751
_RS04805
Position (kb) 912
913
914 Strain fitness (log2 ratio) -2
-1
0
1 at 911.563 kb on - strand, within MMP_RS04785 at 911.564 kb on + strand, within MMP_RS04785 at 911.602 kb on - strand, within MMP_RS04785 at 911.603 kb on + strand, within MMP_RS04785 at 911.603 kb on + strand, within MMP_RS04785 at 911.607 kb on - strand, within MMP_RS04785 at 911.610 kb on + strand, within MMP_RS04785 at 911.615 kb on + strand, within MMP_RS04785 at 911.689 kb on + strand at 911.700 kb on + strand at 911.811 kb on - strand at 911.904 kb on - strand, within MMP_RS04790 at 911.908 kb on - strand, within MMP_RS04790 at 912.140 kb on + strand, within MMP_RS04790 at 912.140 kb on + strand, within MMP_RS04790 at 912.140 kb on + strand, within MMP_RS04790 at 912.141 kb on - strand, within MMP_RS04790 at 912.249 kb on - strand, within MMP_RS04790 at 912.249 kb on - strand, within MMP_RS04790 at 912.297 kb on - strand, within MMP_RS04790 at 912.297 kb on - strand, within MMP_RS04790 at 912.298 kb on + strand, within MMP_RS04790 at 912.302 kb on - strand, within MMP_RS04790 at 912.303 kb on + strand, within MMP_RS04790 at 912.360 kb on - strand, within MMP_RS04790 at 912.360 kb on - strand, within MMP_RS04790 at 912.361 kb on + strand, within MMP_RS04790 at 912.393 kb on - strand, within MMP_RS04790 at 912.434 kb on + strand, within MMP_RS04790 at 912.508 kb on - strand at 912.571 kb on + strand at 912.609 kb on + strand at 912.688 kb on + strand, within MMP_RS04795 at 912.721 kb on - strand, within MMP_RS04795 at 912.722 kb on + strand, within MMP_RS04795 at 912.722 kb on + strand, within MMP_RS04795 at 912.722 kb on + strand, within MMP_RS04795 at 912.722 kb on + strand, within MMP_RS04795 at 912.859 kb on + strand, within MMP_RS04795 at 912.871 kb on - strand, within MMP_RS04795 at 912.901 kb on + strand, within MMP_RS04795 at 912.937 kb on - strand, within MMP_RS04795 at 912.978 kb on - strand, within MMP_RS04795 at 913.119 kb on + strand at 913.132 kb on + strand at 913.135 kb on + strand at 913.135 kb on + strand at 913.136 kb on - strand at 913.136 kb on - strand at 913.137 kb on + strand at 913.139 kb on - strand at 913.221 kb on - strand, within MMP_RS04800 at 913.248 kb on - strand, within MMP_RS04800 at 913.289 kb on - strand, within MMP_RS04800 at 913.290 kb on + strand, within MMP_RS04800 at 913.290 kb on + strand, within MMP_RS04800 at 913.290 kb on + strand, within MMP_RS04800 at 913.304 kb on + strand, within MMP_RS04800 at 913.342 kb on + strand, within MMP_RS04800 at 913.342 kb on - strand, within MMP_RS04800 at 913.401 kb on - strand, within MMP_RS04800 at 913.401 kb on - strand, within MMP_RS04800 at 913.438 kb on + strand, within MMP_RS04800 at 913.462 kb on + strand, within MMP_RS04800 at 913.555 kb on - strand, within MMP_RS04800 at 913.608 kb on + strand at 913.630 kb on - strand at 913.630 kb on - strand at 913.631 kb on + strand at 913.673 kb on - strand at 913.680 kb on + strand at 913.808 kb on - strand, within MMP_RS04805 at 913.809 kb on + strand, within MMP_RS04805 at 913.813 kb on + strand, within MMP_RS04805 at 913.854 kb on - strand, within MMP_RS04805 at 913.889 kb on - strand, within MMP_RS04805 at 914.001 kb on - strand, within MMP_RS04805 at 914.057 kb on - strand, within MMP_RS04805
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin remove 911,563 - MMP_RS04785 0.75 +0.6 911,564 + MMP_RS04785 0.75 -1.4 911,602 - MMP_RS04785 0.81 -0.5 911,603 + MMP_RS04785 0.81 -1.0 911,603 + MMP_RS04785 0.81 +0.3 911,607 - MMP_RS04785 0.82 -1.7 911,610 + MMP_RS04785 0.82 +0.5 911,615 + MMP_RS04785 0.83 +0.3 911,689 + +1.4 911,700 + -0.9 911,811 - +0.8 911,904 - MMP_RS04790 0.21 +0.9 911,908 - MMP_RS04790 0.22 -0.9 912,140 + MMP_RS04790 0.50 -0.1 912,140 + MMP_RS04790 0.50 -0.6 912,140 + MMP_RS04790 0.50 -1.1 912,141 - MMP_RS04790 0.50 -0.2 912,249 - MMP_RS04790 0.64 +0.2 912,249 - MMP_RS04790 0.64 -0.0 912,297 - MMP_RS04790 0.69 -0.6 912,297 - MMP_RS04790 0.69 -0.7 912,298 + MMP_RS04790 0.70 +0.1 912,302 - MMP_RS04790 0.70 -0.9 912,303 + MMP_RS04790 0.70 +0.2 912,360 - MMP_RS04790 0.77 +1.6 912,360 - MMP_RS04790 0.77 -0.9 912,361 + MMP_RS04790 0.77 +0.3 912,393 - MMP_RS04790 0.81 +0.2 912,434 + MMP_RS04790 0.86 -0.5 912,508 - -0.8 912,571 + -1.5 912,609 + -0.6 912,688 + MMP_RS04795 0.22 -0.6 912,721 - MMP_RS04795 0.28 -0.1 912,722 + MMP_RS04795 0.28 -0.9 912,722 + MMP_RS04795 0.28 -1.8 912,722 + MMP_RS04795 0.28 -0.2 912,722 + MMP_RS04795 0.28 +0.1 912,859 + MMP_RS04795 0.51 -1.1 912,871 - MMP_RS04795 0.53 +0.0 912,901 + MMP_RS04795 0.58 +0.3 912,937 - MMP_RS04795 0.64 +0.6 912,978 - MMP_RS04795 0.70 -1.0 913,119 + +0.5 913,132 + -1.1 913,135 + -0.2 913,135 + +0.0 913,136 - -1.5 913,136 - +1.6 913,137 + -0.1 913,139 - -0.0 913,221 - MMP_RS04800 0.12 +1.6 913,248 - MMP_RS04800 0.18 +0.4 913,289 - MMP_RS04800 0.28 -0.8 913,290 + MMP_RS04800 0.28 -0.7 913,290 + MMP_RS04800 0.28 -0.5 913,290 + MMP_RS04800 0.28 -0.7 913,304 + MMP_RS04800 0.31 -0.0 913,342 + MMP_RS04800 0.40 -0.6 913,342 - MMP_RS04800 0.40 -0.7 913,401 - MMP_RS04800 0.53 +1.6 913,401 - MMP_RS04800 0.53 -0.1 913,438 + MMP_RS04800 0.61 +0.9 913,462 + MMP_RS04800 0.67 -0.2 913,555 - MMP_RS04800 0.88 -0.1 913,608 + -0.4 913,630 - +0.2 913,630 - +0.1 913,631 + +0.1 913,673 - -0.3 913,680 + +0.2 913,808 - MMP_RS04805 0.14 -0.3 913,809 + MMP_RS04805 0.14 -0.6 913,813 + MMP_RS04805 0.14 -0.3 913,854 - MMP_RS04805 0.18 +0.5 913,889 - MMP_RS04805 0.21 +0.4 914,001 - MMP_RS04805 0.32 -0.2 914,057 - MMP_RS04805 0.37 -0.3
Or see this region's nucleotide sequence