Experiment: H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04405 and MMP_RS04410 are separated by 50 nucleotides MMP_RS04410 and MMP_RS04415 are separated by 82 nucleotides MMP_RS04415 and MMP_RS04420 are separated by 254 nucleotides MMP_RS04420 and MMP_RS04425 are separated by 62 nucleotides
MMP_RS04405: MMP_RS04405 - hypothetical protein, at 838,896 to 839,165
_RS04405
MMP_RS04410: MMP_RS04410 - hypothetical protein, at 839,216 to 839,815
_RS04410
MMP_RS04415: MMP_RS04415 - peptide-methionine (S)-S-oxide reductase MsrA, at 839,898 to 840,371
_RS04415
MMP_RS04420: MMP_RS04420 - LysE family translocator, at 840,626 to 841,249
_RS04420
MMP_RS04425: MMP_RS04425 - APC family permease, at 841,312 to 842,565
_RS04425
Position (kb) 839
840
841 Strain fitness (log2 ratio) -1
0
1
2 at 838.903 kb on + strand at 838.927 kb on + strand, within MMP_RS04405 at 838.943 kb on + strand, within MMP_RS04405 at 838.990 kb on - strand, within MMP_RS04405 at 838.993 kb on - strand, within MMP_RS04405 at 839.004 kb on + strand, within MMP_RS04405 at 839.016 kb on - strand, within MMP_RS04405 at 839.026 kb on - strand, within MMP_RS04405 at 839.049 kb on - strand, within MMP_RS04405 at 839.050 kb on + strand, within MMP_RS04405 at 839.065 kb on - strand, within MMP_RS04405 at 839.076 kb on + strand, within MMP_RS04405 at 839.138 kb on - strand, within MMP_RS04405 at 839.169 kb on + strand at 839.174 kb on + strand at 839.263 kb on + strand at 839.599 kb on - strand, within MMP_RS04410 at 839.620 kb on - strand, within MMP_RS04410 at 839.643 kb on + strand, within MMP_RS04410 at 839.663 kb on - strand, within MMP_RS04410 at 839.748 kb on - strand, within MMP_RS04410 at 839.819 kb on + strand at 839.843 kb on + strand at 839.849 kb on - strand at 839.850 kb on + strand at 839.863 kb on - strand at 839.870 kb on + strand at 839.876 kb on + strand at 839.919 kb on + strand at 839.936 kb on + strand at 839.936 kb on + strand at 840.045 kb on - strand, within MMP_RS04415 at 840.045 kb on - strand, within MMP_RS04415 at 840.046 kb on + strand, within MMP_RS04415 at 840.086 kb on + strand, within MMP_RS04415 at 840.122 kb on - strand, within MMP_RS04415 at 840.122 kb on - strand, within MMP_RS04415 at 840.123 kb on + strand, within MMP_RS04415 at 840.148 kb on + strand, within MMP_RS04415 at 840.153 kb on - strand, within MMP_RS04415 at 840.156 kb on - strand, within MMP_RS04415 at 840.157 kb on + strand, within MMP_RS04415 at 840.172 kb on + strand, within MMP_RS04415 at 840.193 kb on + strand, within MMP_RS04415 at 840.197 kb on - strand, within MMP_RS04415 at 840.360 kb on + strand at 840.362 kb on - strand at 840.392 kb on - strand at 840.393 kb on + strand at 840.422 kb on + strand at 840.458 kb on - strand at 840.463 kb on + strand at 840.463 kb on + strand at 840.485 kb on - strand at 840.486 kb on + strand at 840.487 kb on - strand at 840.489 kb on - strand at 840.490 kb on + strand at 840.490 kb on + strand at 840.501 kb on - strand at 840.502 kb on + strand at 840.502 kb on + strand at 840.510 kb on + strand at 840.520 kb on - strand at 840.582 kb on + strand at 840.590 kb on - strand at 840.590 kb on - strand at 840.590 kb on - strand at 840.590 kb on - strand at 840.591 kb on + strand at 840.595 kb on + strand at 840.599 kb on + strand at 840.637 kb on - strand at 840.650 kb on - strand at 840.676 kb on - strand at 840.676 kb on - strand at 840.677 kb on + strand at 840.677 kb on + strand at 840.699 kb on - strand, within MMP_RS04420 at 840.791 kb on + strand, within MMP_RS04420 at 840.937 kb on - strand, within MMP_RS04420 at 841.071 kb on - strand, within MMP_RS04420 at 841.155 kb on - strand, within MMP_RS04420 at 841.182 kb on + strand, within MMP_RS04420 at 841.300 kb on + strand at 841.311 kb on - strand at 841.311 kb on - strand at 841.311 kb on - strand at 841.337 kb on + strand at 841.338 kb on - strand at 841.338 kb on - strand at 841.339 kb on + strand at 841.339 kb on + strand at 841.343 kb on - strand at 841.344 kb on + strand at 841.353 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin remove 838,903 + +0.6 838,927 + MMP_RS04405 0.11 +0.1 838,943 + MMP_RS04405 0.17 -1.0 838,990 - MMP_RS04405 0.35 -0.1 838,993 - MMP_RS04405 0.36 +0.3 839,004 + MMP_RS04405 0.40 -0.0 839,016 - MMP_RS04405 0.44 +1.1 839,026 - MMP_RS04405 0.48 -1.0 839,049 - MMP_RS04405 0.57 +2.6 839,050 + MMP_RS04405 0.57 +0.6 839,065 - MMP_RS04405 0.63 -0.9 839,076 + MMP_RS04405 0.67 +0.1 839,138 - MMP_RS04405 0.90 -0.2 839,169 + +0.1 839,174 + +0.6 839,263 + +0.5 839,599 - MMP_RS04410 0.64 -1.2 839,620 - MMP_RS04410 0.67 +0.2 839,643 + MMP_RS04410 0.71 -0.3 839,663 - MMP_RS04410 0.74 +0.2 839,748 - MMP_RS04410 0.89 -0.4 839,819 + -0.8 839,843 + -0.3 839,849 - -0.2 839,850 + -0.3 839,863 - -0.5 839,870 + -0.1 839,876 + -0.2 839,919 + -0.3 839,936 + -0.2 839,936 + +0.7 840,045 - MMP_RS04415 0.31 +0.7 840,045 - MMP_RS04415 0.31 +0.2 840,046 + MMP_RS04415 0.31 +0.0 840,086 + MMP_RS04415 0.40 -0.0 840,122 - MMP_RS04415 0.47 -0.4 840,122 - MMP_RS04415 0.47 -0.7 840,123 + MMP_RS04415 0.47 -1.0 840,148 + MMP_RS04415 0.53 -0.6 840,153 - MMP_RS04415 0.54 +0.4 840,156 - MMP_RS04415 0.54 -0.7 840,157 + MMP_RS04415 0.55 +0.3 840,172 + MMP_RS04415 0.58 -0.8 840,193 + MMP_RS04415 0.62 +1.1 840,197 - MMP_RS04415 0.63 -1.1 840,360 + +1.7 840,362 - +0.7 840,392 - -0.2 840,393 + +0.3 840,422 + +0.1 840,458 - -1.0 840,463 + -0.1 840,463 + -0.3 840,485 - -0.8 840,486 + +0.1 840,487 - -0.8 840,489 - -0.5 840,490 + +0.1 840,490 + -0.2 840,501 - -1.3 840,502 + +1.5 840,502 + -0.0 840,510 + -0.0 840,520 - -0.6 840,582 + -0.7 840,590 - -0.1 840,590 - +0.2 840,590 - -0.4 840,590 - +1.3 840,591 + -0.3 840,595 + -0.7 840,599 + -0.8 840,637 - -0.1 840,650 - +0.7 840,676 - -0.6 840,676 - +0.1 840,677 + -0.5 840,677 + -0.2 840,699 - MMP_RS04420 0.12 +0.9 840,791 + MMP_RS04420 0.26 -1.4 840,937 - MMP_RS04420 0.50 -0.7 841,071 - MMP_RS04420 0.71 -1.3 841,155 - MMP_RS04420 0.85 -0.5 841,182 + MMP_RS04420 0.89 -0.4 841,300 + +1.0 841,311 - +0.4 841,311 - +1.2 841,311 - -0.8 841,337 + -0.3 841,338 - +0.7 841,338 - -1.4 841,339 + +0.6 841,339 + -0.5 841,343 - -0.2 841,344 + +0.2 841,353 - +0.5
Or see this region's nucleotide sequence