Experiment: H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03945 and MMP_RS03950 are separated by 2 nucleotides MMP_RS03950 and MMP_RS03955 are separated by 19 nucleotides MMP_RS03955 and MMP_RS03960 are separated by 196 nucleotides MMP_RS03960 and MMP_RS03965 are separated by 168 nucleotides
MMP_RS03945: MMP_RS03945 - nucleoside triphosphate pyrophosphohydrolase, at 749,567 to 749,875
_RS03945
MMP_RS03950: MMP_RS03950 - HNH endonuclease, at 749,878 to 750,849
_RS03950
MMP_RS03955: MMP_RS03955 - DUF3578 domain-containing protein, at 750,869 to 752,053
_RS03955
MMP_RS03960: MMP_RS03960 - hypothetical protein, at 752,250 to 752,480
_RS03960
MMP_RS03965: MMP_RS03965 - hypothetical protein, at 752,649 to 753,023
_RS03965
Position (kb)
750
751
752
753 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 749.876 kb on + strand at 749.876 kb on + strand at 749.908 kb on + strand at 749.913 kb on + strand at 749.913 kb on + strand at 749.935 kb on - strand at 749.936 kb on + strand at 750.102 kb on + strand, within MMP_RS03950 at 750.105 kb on - strand, within MMP_RS03950 at 750.193 kb on + strand, within MMP_RS03950 at 750.200 kb on + strand, within MMP_RS03950 at 750.269 kb on + strand, within MMP_RS03950 at 750.582 kb on + strand, within MMP_RS03950 at 750.584 kb on - strand, within MMP_RS03950 at 750.608 kb on - strand, within MMP_RS03950 at 750.662 kb on - strand, within MMP_RS03950 at 750.694 kb on - strand, within MMP_RS03950 at 750.695 kb on + strand, within MMP_RS03950 at 750.816 kb on - strand at 750.883 kb on + strand at 750.883 kb on + strand at 750.930 kb on + strand at 750.937 kb on + strand at 750.981 kb on - strand at 750.981 kb on - strand at 750.982 kb on + strand at 750.983 kb on - strand at 750.983 kb on - strand at 750.983 kb on - strand at 750.990 kb on - strand, within MMP_RS03955 at 750.991 kb on + strand, within MMP_RS03955 at 751.083 kb on - strand, within MMP_RS03955 at 751.083 kb on - strand, within MMP_RS03955 at 751.084 kb on + strand, within MMP_RS03955 at 751.084 kb on + strand, within MMP_RS03955 at 751.084 kb on + strand, within MMP_RS03955 at 751.161 kb on + strand, within MMP_RS03955 at 751.205 kb on - strand, within MMP_RS03955 at 751.218 kb on - strand, within MMP_RS03955 at 751.253 kb on + strand, within MMP_RS03955 at 751.253 kb on + strand, within MMP_RS03955 at 751.427 kb on - strand, within MMP_RS03955 at 751.427 kb on - strand, within MMP_RS03955 at 751.427 kb on - strand, within MMP_RS03955 at 751.670 kb on + strand, within MMP_RS03955 at 751.761 kb on - strand, within MMP_RS03955 at 751.799 kb on + strand, within MMP_RS03955 at 751.801 kb on + strand, within MMP_RS03955 at 751.801 kb on + strand, within MMP_RS03955 at 751.801 kb on + strand, within MMP_RS03955 at 751.801 kb on + strand, within MMP_RS03955 at 751.842 kb on + strand, within MMP_RS03955 at 751.842 kb on + strand, within MMP_RS03955 at 751.953 kb on + strand at 752.006 kb on + strand at 752.006 kb on + strand at 752.007 kb on - strand at 752.016 kb on + strand at 752.039 kb on - strand at 752.039 kb on - strand at 752.039 kb on - strand at 752.071 kb on + strand at 752.090 kb on - strand at 752.098 kb on - strand at 752.099 kb on + strand at 752.101 kb on - strand at 752.101 kb on - strand at 752.102 kb on + strand at 752.169 kb on + strand at 752.171 kb on - strand at 752.172 kb on + strand at 752.178 kb on + strand at 752.236 kb on - strand at 752.376 kb on - strand, within MMP_RS03960 at 752.526 kb on + strand at 752.528 kb on - strand at 752.531 kb on - strand at 752.534 kb on - strand at 752.535 kb on + strand at 752.776 kb on - strand, within MMP_RS03965 at 752.987 kb on - strand at 752.987 kb on - strand at 752.998 kb on + strand at 753.012 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin remove 749,876 + -0.6 749,876 + -0.3 749,908 + +0.9 749,913 + +3.6 749,913 + -1.2 749,935 - +0.3 749,936 + -0.1 750,102 + MMP_RS03950 0.23 +0.4 750,105 - MMP_RS03950 0.23 -0.1 750,193 + MMP_RS03950 0.32 +0.0 750,200 + MMP_RS03950 0.33 +1.2 750,269 + MMP_RS03950 0.40 -0.4 750,582 + MMP_RS03950 0.72 -0.2 750,584 - MMP_RS03950 0.73 +0.4 750,608 - MMP_RS03950 0.75 +0.2 750,662 - MMP_RS03950 0.81 +0.3 750,694 - MMP_RS03950 0.84 -0.3 750,695 + MMP_RS03950 0.84 -0.5 750,816 - -0.4 750,883 + +0.9 750,883 + +0.1 750,930 + +0.1 750,937 + -0.3 750,981 - +0.6 750,981 - +0.3 750,982 + -0.7 750,983 - -0.2 750,983 - +0.6 750,983 - -0.5 750,990 - MMP_RS03955 0.10 +0.1 750,991 + MMP_RS03955 0.10 -0.8 751,083 - MMP_RS03955 0.18 -0.1 751,083 - MMP_RS03955 0.18 +0.6 751,084 + MMP_RS03955 0.18 -1.0 751,084 + MMP_RS03955 0.18 -0.3 751,084 + MMP_RS03955 0.18 -0.0 751,161 + MMP_RS03955 0.25 -0.9 751,205 - MMP_RS03955 0.28 +0.0 751,218 - MMP_RS03955 0.29 -1.3 751,253 + MMP_RS03955 0.32 +1.4 751,253 + MMP_RS03955 0.32 -0.5 751,427 - MMP_RS03955 0.47 -1.5 751,427 - MMP_RS03955 0.47 -2.0 751,427 - MMP_RS03955 0.47 +0.2 751,670 + MMP_RS03955 0.68 -0.1 751,761 - MMP_RS03955 0.75 -1.0 751,799 + MMP_RS03955 0.78 +0.2 751,801 + MMP_RS03955 0.79 -1.1 751,801 + MMP_RS03955 0.79 -1.2 751,801 + MMP_RS03955 0.79 -0.9 751,801 + MMP_RS03955 0.79 +0.2 751,842 + MMP_RS03955 0.82 -1.2 751,842 + MMP_RS03955 0.82 -1.6 751,953 + -0.1 752,006 + -0.1 752,006 + +1.8 752,007 - +0.2 752,016 + -0.4 752,039 - +0.2 752,039 - -0.8 752,039 - +1.0 752,071 + +0.1 752,090 - -0.4 752,098 - -0.4 752,099 + -0.3 752,101 - -0.1 752,101 - -0.1 752,102 + +0.3 752,169 + -1.9 752,171 - -0.9 752,172 + -0.9 752,178 + -0.5 752,236 - +0.6 752,376 - MMP_RS03960 0.55 -2.2 752,526 + -0.5 752,528 - +1.6 752,531 - +0.1 752,534 - +0.0 752,535 + +0.2 752,776 - MMP_RS03965 0.34 -0.7 752,987 - -0.5 752,987 - -0.7 752,998 + +0.5 753,012 - -0.3
Or see this region's nucleotide sequence