Experiment: H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03700 and MMP_RS03705 are separated by 204 nucleotides MMP_RS03705 and MMP_RS03710 are separated by 157 nucleotides MMP_RS03710 and MMP_RS03715 are separated by 216 nucleotides
MMP_RS03700: MMP_RS03700 - sodium:proton antiporter, at 697,771 to 699,024
_RS03700
MMP_RS03705: MMP_RS03705 - (5-formylfuran-3-yl)methyl phosphate synthase, at 699,229 to 699,939
_RS03705
MMP_RS03710: MMP_RS03710 - tRNA-Arg, at 700,097 to 700,170
_RS03710
MMP_RS03715: MMP_RS03715 - class III signal peptide-containing protein, at 700,387 to 700,983
_RS03715
Position (kb) 699
700 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8
9
10
11 at 698.235 kb on - strand, within MMP_RS03700 at 698.256 kb on + strand, within MMP_RS03700 at 698.256 kb on + strand, within MMP_RS03700 at 698.257 kb on - strand, within MMP_RS03700 at 698.363 kb on + strand, within MMP_RS03700 at 698.363 kb on + strand, within MMP_RS03700 at 698.551 kb on - strand, within MMP_RS03700 at 698.616 kb on - strand, within MMP_RS03700 at 698.617 kb on + strand, within MMP_RS03700 at 698.617 kb on + strand, within MMP_RS03700 at 698.617 kb on + strand, within MMP_RS03700 at 698.654 kb on - strand, within MMP_RS03700 at 698.658 kb on - strand, within MMP_RS03700 at 698.676 kb on - strand, within MMP_RS03700 at 698.676 kb on - strand, within MMP_RS03700 at 698.677 kb on + strand, within MMP_RS03700 at 698.690 kb on - strand, within MMP_RS03700 at 698.690 kb on - strand, within MMP_RS03700 at 698.691 kb on + strand, within MMP_RS03700 at 698.691 kb on + strand, within MMP_RS03700 at 698.691 kb on + strand, within MMP_RS03700 at 698.691 kb on + strand, within MMP_RS03700 at 698.692 kb on - strand, within MMP_RS03700 at 698.693 kb on + strand, within MMP_RS03700 at 698.693 kb on + strand, within MMP_RS03700 at 698.694 kb on - strand, within MMP_RS03700 at 698.768 kb on + strand, within MMP_RS03700 at 698.903 kb on + strand at 698.913 kb on - strand at 698.945 kb on + strand at 698.945 kb on + strand at 698.965 kb on - strand at 698.999 kb on - strand at 699.038 kb on + strand at 699.041 kb on - strand at 699.047 kb on + strand at 699.048 kb on - strand at 699.048 kb on - strand at 699.049 kb on + strand at 699.075 kb on - strand at 699.106 kb on - strand at 699.106 kb on - strand at 699.113 kb on - strand at 699.134 kb on + strand at 699.171 kb on - strand at 699.176 kb on + strand at 699.176 kb on + strand at 699.182 kb on + strand at 699.186 kb on + strand at 699.186 kb on + strand at 699.197 kb on - strand at 699.298 kb on + strand at 699.350 kb on - strand at 699.462 kb on + strand at 699.462 kb on + strand at 699.795 kb on - strand at 699.819 kb on + strand at 699.827 kb on - strand at 699.827 kb on - strand at 699.827 kb on - strand at 699.864 kb on - strand at 699.955 kb on + strand at 699.968 kb on + strand at 699.977 kb on + strand at 700.050 kb on - strand at 700.051 kb on + strand at 700.051 kb on + strand at 700.177 kb on - strand at 700.290 kb on - strand at 700.290 kb on - strand at 700.298 kb on + strand at 700.298 kb on + strand at 700.307 kb on + strand at 700.310 kb on + strand at 700.317 kb on + strand at 700.351 kb on - strand at 700.354 kb on + strand at 700.358 kb on - strand at 700.358 kb on - strand at 700.359 kb on + strand at 700.359 kb on + strand at 700.362 kb on - strand at 700.362 kb on - strand at 700.363 kb on + strand at 700.363 kb on + strand at 700.364 kb on - strand at 700.364 kb on - strand at 700.364 kb on - strand at 700.364 kb on - strand at 700.364 kb on - strand at 700.365 kb on + strand at 700.365 kb on + strand at 700.365 kb on + strand at 700.428 kb on + strand at 700.456 kb on - strand, within MMP_RS03715 at 700.648 kb on - strand, within MMP_RS03715 at 700.648 kb on - strand, within MMP_RS03715 at 700.648 kb on - strand, within MMP_RS03715 at 700.649 kb on + strand, within MMP_RS03715 at 700.649 kb on + strand, within MMP_RS03715 at 700.649 kb on + strand, within MMP_RS03715 at 700.649 kb on + strand, within MMP_RS03715 at 700.653 kb on - strand, within MMP_RS03715 at 700.654 kb on + strand, within MMP_RS03715 at 700.654 kb on + strand, within MMP_RS03715 at 700.656 kb on - strand, within MMP_RS03715 at 700.674 kb on + strand, within MMP_RS03715 at 700.698 kb on + strand, within MMP_RS03715 at 700.698 kb on + strand, within MMP_RS03715 at 700.698 kb on + strand, within MMP_RS03715 at 700.811 kb on + strand, within MMP_RS03715 at 700.838 kb on - strand, within MMP_RS03715 at 700.934 kb on + strand at 700.938 kb on + strand at 700.938 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin remove 698,235 - MMP_RS03700 0.37 +1.0 698,256 + MMP_RS03700 0.39 +0.8 698,256 + MMP_RS03700 0.39 +1.0 698,257 - MMP_RS03700 0.39 +2.0 698,363 + MMP_RS03700 0.47 +2.0 698,363 + MMP_RS03700 0.47 -0.3 698,551 - MMP_RS03700 0.62 +8.0 698,616 - MMP_RS03700 0.67 +7.5 698,617 + MMP_RS03700 0.67 +10.0 698,617 + MMP_RS03700 0.67 +8.2 698,617 + MMP_RS03700 0.67 +9.1 698,654 - MMP_RS03700 0.70 +3.9 698,658 - MMP_RS03700 0.71 +7.5 698,676 - MMP_RS03700 0.72 +7.3 698,676 - MMP_RS03700 0.72 +7.0 698,677 + MMP_RS03700 0.72 +8.6 698,690 - MMP_RS03700 0.73 +6.9 698,690 - MMP_RS03700 0.73 +6.7 698,691 + MMP_RS03700 0.73 +8.2 698,691 + MMP_RS03700 0.73 +8.6 698,691 + MMP_RS03700 0.73 +7.6 698,691 + MMP_RS03700 0.73 +7.1 698,692 - MMP_RS03700 0.73 +7.1 698,693 + MMP_RS03700 0.74 +8.9 698,693 + MMP_RS03700 0.74 +7.9 698,694 - MMP_RS03700 0.74 +7.3 698,768 + MMP_RS03700 0.80 +8.7 698,903 + +9.6 698,913 - +7.1 698,945 + +9.4 698,945 + +10.9 698,965 - +6.5 698,999 - +8.3 699,038 + +8.5 699,041 - +5.5 699,047 + +5.9 699,048 - +2.9 699,048 - +5.2 699,049 + +9.6 699,075 - +2.9 699,106 - +1.7 699,106 - +2.8 699,113 - +2.7 699,134 + +8.0 699,171 - +0.8 699,176 + -0.2 699,176 + -0.1 699,182 + -1.1 699,186 + +1.6 699,186 + +0.2 699,197 - +0.1 699,298 + +3.6 699,350 - +1.8 699,462 + -0.9 699,462 + -0.1 699,795 - +1.3 699,819 + -0.1 699,827 - -0.2 699,827 - -0.7 699,827 - +1.0 699,864 - -0.3 699,955 + +0.2 699,968 + -0.1 699,977 + -1.5 700,050 - -0.6 700,051 + +0.0 700,051 + +0.0 700,177 - -0.8 700,290 - -0.1 700,290 - -0.5 700,298 + +1.8 700,298 + +0.4 700,307 + +0.1 700,310 + +0.5 700,317 + -0.6 700,351 - -0.1 700,354 + -0.2 700,358 - +0.7 700,358 - +0.9 700,359 + -0.7 700,359 + +0.1 700,362 - +0.2 700,362 - +0.2 700,363 + -0.7 700,363 + +0.9 700,364 - +0.3 700,364 - -0.3 700,364 - -0.4 700,364 - -0.9 700,364 - -0.1 700,365 + -0.3 700,365 + -0.1 700,365 + -0.0 700,428 + -0.7 700,456 - MMP_RS03715 0.12 -0.1 700,648 - MMP_RS03715 0.44 +0.0 700,648 - MMP_RS03715 0.44 +0.1 700,648 - MMP_RS03715 0.44 +0.0 700,649 + MMP_RS03715 0.44 -0.9 700,649 + MMP_RS03715 0.44 -0.0 700,649 + MMP_RS03715 0.44 -0.2 700,649 + MMP_RS03715 0.44 +0.2 700,653 - MMP_RS03715 0.45 +1.0 700,654 + MMP_RS03715 0.45 -0.4 700,654 + MMP_RS03715 0.45 +0.6 700,656 - MMP_RS03715 0.45 +0.3 700,674 + MMP_RS03715 0.48 +0.8 700,698 + MMP_RS03715 0.52 +1.5 700,698 + MMP_RS03715 0.52 +0.6 700,698 + MMP_RS03715 0.52 -1.8 700,811 + MMP_RS03715 0.71 +0.1 700,838 - MMP_RS03715 0.76 +0.0 700,934 + +0.0 700,938 + -0.3 700,938 + -0.5
Or see this region's nucleotide sequence