Experiment: H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03690 and MMP_RS03695 are separated by 4 nucleotides MMP_RS03695 and MMP_RS03700 are separated by 12 nucleotides
MMP_RS03690: MMP_RS03690 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 695,370 to 696,470
_RS03690
MMP_RS03695: MMP_RS03695 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 696,475 to 697,758
_RS03695
MMP_RS03700: MMP_RS03700 - sodium:proton antiporter, at 697,771 to 699,024
_RS03700
Position (kb) 696
697
698 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7
8
9
10 at 695.623 kb on + strand at 695.623 kb on + strand at 695.625 kb on + strand at 695.786 kb on - strand at 695.790 kb on - strand at 695.791 kb on + strand at 695.795 kb on - strand at 695.795 kb on - strand at 695.795 kb on - strand at 695.978 kb on - strand at 695.978 kb on - strand at 695.983 kb on + strand at 696.189 kb on + strand at 696.189 kb on + strand at 696.756 kb on - strand at 696.787 kb on + strand at 696.857 kb on + strand at 696.884 kb on - strand at 696.973 kb on - strand at 696.974 kb on + strand at 697.175 kb on + strand at 697.317 kb on + strand at 697.372 kb on + strand at 697.382 kb on + strand at 697.402 kb on - strand at 697.556 kb on + strand at 697.614 kb on - strand at 697.889 kb on - strand at 697.932 kb on - strand, within MMP_RS03700 at 697.940 kb on - strand, within MMP_RS03700 at 697.941 kb on + strand, within MMP_RS03700 at 698.235 kb on - strand, within MMP_RS03700 at 698.256 kb on + strand, within MMP_RS03700 at 698.256 kb on + strand, within MMP_RS03700 at 698.257 kb on - strand, within MMP_RS03700 at 698.363 kb on + strand, within MMP_RS03700 at 698.363 kb on + strand, within MMP_RS03700 at 698.551 kb on - strand, within MMP_RS03700 at 698.616 kb on - strand, within MMP_RS03700 at 698.617 kb on + strand, within MMP_RS03700 at 698.617 kb on + strand, within MMP_RS03700 at 698.617 kb on + strand, within MMP_RS03700 at 698.654 kb on - strand, within MMP_RS03700 at 698.658 kb on - strand, within MMP_RS03700 at 698.676 kb on - strand, within MMP_RS03700 at 698.676 kb on - strand, within MMP_RS03700 at 698.677 kb on + strand, within MMP_RS03700 at 698.690 kb on - strand, within MMP_RS03700 at 698.690 kb on - strand, within MMP_RS03700 at 698.691 kb on + strand, within MMP_RS03700 at 698.691 kb on + strand, within MMP_RS03700 at 698.691 kb on + strand, within MMP_RS03700 at 698.691 kb on + strand, within MMP_RS03700 at 698.692 kb on - strand, within MMP_RS03700 at 698.693 kb on + strand, within MMP_RS03700 at 698.693 kb on + strand, within MMP_RS03700 at 698.694 kb on - strand, within MMP_RS03700
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin remove 695,623 + +1.3 695,623 + +0.6 695,625 + +2.6 695,786 - +2.6 695,790 - -0.3 695,791 + +3.6 695,795 - +1.6 695,795 - +3.6 695,795 - +1.3 695,978 - -0.1 695,978 - +2.6 695,983 + +0.4 696,189 + +0.4 696,189 + +0.3 696,756 - -0.1 696,787 + +1.3 696,857 + +2.6 696,884 - +0.8 696,973 - +0.6 696,974 + +2.0 697,175 + +3.6 697,317 + +1.3 697,372 + +2.0 697,382 + +1.6 697,402 - +1.0 697,556 + +0.3 697,614 - +1.3 697,889 - +0.3 697,932 - MMP_RS03700 0.13 +1.6 697,940 - MMP_RS03700 0.13 +0.4 697,941 + MMP_RS03700 0.14 +3.6 698,235 - MMP_RS03700 0.37 +1.0 698,256 + MMP_RS03700 0.39 +0.8 698,256 + MMP_RS03700 0.39 +1.0 698,257 - MMP_RS03700 0.39 +2.0 698,363 + MMP_RS03700 0.47 +2.0 698,363 + MMP_RS03700 0.47 -0.3 698,551 - MMP_RS03700 0.62 +8.0 698,616 - MMP_RS03700 0.67 +7.5 698,617 + MMP_RS03700 0.67 +10.0 698,617 + MMP_RS03700 0.67 +8.2 698,617 + MMP_RS03700 0.67 +9.1 698,654 - MMP_RS03700 0.70 +3.9 698,658 - MMP_RS03700 0.71 +7.5 698,676 - MMP_RS03700 0.72 +7.3 698,676 - MMP_RS03700 0.72 +7.0 698,677 + MMP_RS03700 0.72 +8.6 698,690 - MMP_RS03700 0.73 +6.9 698,690 - MMP_RS03700 0.73 +6.7 698,691 + MMP_RS03700 0.73 +8.2 698,691 + MMP_RS03700 0.73 +8.6 698,691 + MMP_RS03700 0.73 +7.6 698,691 + MMP_RS03700 0.73 +7.1 698,692 - MMP_RS03700 0.73 +7.1 698,693 + MMP_RS03700 0.74 +8.9 698,693 + MMP_RS03700 0.74 +7.9 698,694 - MMP_RS03700 0.74 +7.3
Or see this region's nucleotide sequence