Experiment: H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02445 and MMP_RS02450 are separated by 273 nucleotides MMP_RS02450 and MMP_RS02455 are separated by 12 nucleotides MMP_RS02455 and MMP_RS02460 are separated by 165 nucleotides
MMP_RS02445: MMP_RS02445 - putative DNA binding domain-containing protein, at 456,701 to 458,044
_RS02445
MMP_RS02450: MMP_RS02450 - hypothetical protein, at 458,318 to 458,512
_RS02450
MMP_RS02455: MMP_RS02455 - hypothetical protein, at 458,525 to 459,142
_RS02455
MMP_RS02460: MMP_RS02460 - hypothetical protein, at 459,308 to 462,367
_RS02460
Position (kb) 458
459 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 457.318 kb on - strand, within MMP_RS02445 at 457.319 kb on + strand, within MMP_RS02445 at 457.334 kb on - strand, within MMP_RS02445 at 457.450 kb on + strand, within MMP_RS02445 at 457.519 kb on - strand, within MMP_RS02445 at 457.560 kb on - strand, within MMP_RS02445 at 457.561 kb on + strand, within MMP_RS02445 at 457.564 kb on - strand, within MMP_RS02445 at 457.564 kb on - strand, within MMP_RS02445 at 457.564 kb on - strand, within MMP_RS02445 at 457.564 kb on - strand, within MMP_RS02445 at 457.564 kb on - strand, within MMP_RS02445 at 457.565 kb on + strand, within MMP_RS02445 at 457.565 kb on + strand, within MMP_RS02445 at 457.566 kb on - strand, within MMP_RS02445 at 457.566 kb on - strand, within MMP_RS02445 at 457.566 kb on - strand, within MMP_RS02445 at 457.567 kb on + strand, within MMP_RS02445 at 457.567 kb on + strand, within MMP_RS02445 at 457.605 kb on - strand, within MMP_RS02445 at 457.606 kb on + strand, within MMP_RS02445 at 457.611 kb on - strand, within MMP_RS02445 at 457.611 kb on - strand, within MMP_RS02445 at 457.622 kb on - strand, within MMP_RS02445 at 457.675 kb on + strand, within MMP_RS02445 at 457.716 kb on - strand, within MMP_RS02445 at 457.879 kb on + strand, within MMP_RS02445 at 457.919 kb on + strand at 457.932 kb on - strand at 457.942 kb on - strand at 457.943 kb on + strand at 457.996 kb on + strand at 457.996 kb on + strand at 458.048 kb on + strand at 458.048 kb on + strand at 458.048 kb on + strand at 458.054 kb on + strand at 458.054 kb on + strand at 458.064 kb on - strand at 458.087 kb on + strand at 458.098 kb on + strand at 458.136 kb on + strand at 458.136 kb on + strand at 458.214 kb on - strand at 458.231 kb on + strand at 458.250 kb on + strand at 458.308 kb on - strand at 458.347 kb on + strand, within MMP_RS02450 at 458.361 kb on - strand, within MMP_RS02450 at 458.371 kb on - strand, within MMP_RS02450 at 458.392 kb on - strand, within MMP_RS02450 at 458.392 kb on - strand, within MMP_RS02450 at 458.398 kb on + strand, within MMP_RS02450 at 458.398 kb on + strand, within MMP_RS02450 at 458.403 kb on + strand, within MMP_RS02450 at 458.468 kb on + strand, within MMP_RS02450 at 458.532 kb on + strand at 458.628 kb on - strand, within MMP_RS02455 at 458.629 kb on + strand, within MMP_RS02455 at 458.630 kb on - strand, within MMP_RS02455 at 458.760 kb on + strand, within MMP_RS02455 at 458.783 kb on - strand, within MMP_RS02455 at 458.787 kb on - strand, within MMP_RS02455 at 458.787 kb on - strand, within MMP_RS02455 at 458.960 kb on + strand, within MMP_RS02455 at 458.963 kb on - strand, within MMP_RS02455 at 458.974 kb on - strand, within MMP_RS02455 at 458.974 kb on - strand, within MMP_RS02455 at 458.996 kb on + strand, within MMP_RS02455 at 459.136 kb on - strand at 459.212 kb on + strand at 459.241 kb on - strand at 459.255 kb on - strand at 459.288 kb on - strand at 459.307 kb on + strand at 459.321 kb on - strand at 459.344 kb on - strand at 459.345 kb on + strand at 459.378 kb on + strand at 459.409 kb on - strand at 459.409 kb on - strand at 459.409 kb on - strand at 459.410 kb on + strand at 459.411 kb on - strand at 459.446 kb on + strand at 459.448 kb on - strand at 459.448 kb on - strand at 459.448 kb on - strand at 459.449 kb on + strand at 459.451 kb on - strand at 459.452 kb on + strand at 459.452 kb on + strand at 459.452 kb on + strand at 459.452 kb on + strand at 459.453 kb on - strand at 459.453 kb on - strand at 459.454 kb on + strand at 459.457 kb on - strand at 459.457 kb on - strand at 459.457 kb on - strand at 459.458 kb on + strand at 459.482 kb on - strand at 459.482 kb on - strand at 459.482 kb on - strand at 459.502 kb on - strand at 459.502 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin remove 457,318 - MMP_RS02445 0.46 +0.7 457,319 + MMP_RS02445 0.46 +0.0 457,334 - MMP_RS02445 0.47 +0.2 457,450 + MMP_RS02445 0.56 -0.3 457,519 - MMP_RS02445 0.61 +0.2 457,560 - MMP_RS02445 0.64 -0.1 457,561 + MMP_RS02445 0.64 -0.8 457,564 - MMP_RS02445 0.64 -0.8 457,564 - MMP_RS02445 0.64 +0.2 457,564 - MMP_RS02445 0.64 -1.4 457,564 - MMP_RS02445 0.64 -0.7 457,564 - MMP_RS02445 0.64 -0.5 457,565 + MMP_RS02445 0.64 +1.2 457,565 + MMP_RS02445 0.64 -1.5 457,566 - MMP_RS02445 0.64 -0.0 457,566 - MMP_RS02445 0.64 +0.5 457,566 - MMP_RS02445 0.64 -0.3 457,567 + MMP_RS02445 0.64 -0.3 457,567 + MMP_RS02445 0.64 -1.1 457,605 - MMP_RS02445 0.67 +0.3 457,606 + MMP_RS02445 0.67 -0.6 457,611 - MMP_RS02445 0.68 -0.0 457,611 - MMP_RS02445 0.68 +0.9 457,622 - MMP_RS02445 0.69 -1.1 457,675 + MMP_RS02445 0.72 -0.9 457,716 - MMP_RS02445 0.76 -0.6 457,879 + MMP_RS02445 0.88 -0.3 457,919 + -0.7 457,932 - +0.1 457,942 - -0.6 457,943 + -1.0 457,996 + -0.2 457,996 + -0.2 458,048 + -1.8 458,048 + +0.5 458,048 + -0.4 458,054 + -1.4 458,054 + -0.4 458,064 - -0.4 458,087 + +0.4 458,098 + -0.8 458,136 + -0.5 458,136 + -0.2 458,214 - -0.3 458,231 + +0.2 458,250 + -1.0 458,308 - +0.3 458,347 + MMP_RS02450 0.15 -0.4 458,361 - MMP_RS02450 0.22 -0.2 458,371 - MMP_RS02450 0.27 -0.9 458,392 - MMP_RS02450 0.38 +0.9 458,392 - MMP_RS02450 0.38 -0.5 458,398 + MMP_RS02450 0.41 -0.3 458,398 + MMP_RS02450 0.41 +6.2 458,403 + MMP_RS02450 0.44 -0.1 458,468 + MMP_RS02450 0.77 +0.3 458,532 + -1.3 458,628 - MMP_RS02455 0.17 +0.6 458,629 + MMP_RS02455 0.17 -0.6 458,630 - MMP_RS02455 0.17 -0.4 458,760 + MMP_RS02455 0.38 -0.2 458,783 - MMP_RS02455 0.42 +0.5 458,787 - MMP_RS02455 0.42 +1.4 458,787 - MMP_RS02455 0.42 +1.5 458,960 + MMP_RS02455 0.70 -1.0 458,963 - MMP_RS02455 0.71 -0.4 458,974 - MMP_RS02455 0.73 +0.1 458,974 - MMP_RS02455 0.73 -0.3 458,996 + MMP_RS02455 0.76 +0.4 459,136 - +1.1 459,212 + -0.6 459,241 - -0.9 459,255 - +0.1 459,288 - -0.1 459,307 + -0.8 459,321 - -1.1 459,344 - -1.5 459,345 + -0.5 459,378 + -0.3 459,409 - -0.5 459,409 - -0.3 459,409 - -0.5 459,410 + -0.3 459,411 - -0.0 459,446 + +1.2 459,448 - +0.8 459,448 - -0.1 459,448 - -0.6 459,449 + -0.1 459,451 - -0.8 459,452 + -0.1 459,452 + +0.5 459,452 + -0.5 459,452 + +0.6 459,453 - -0.8 459,453 - -0.9 459,454 + +0.8 459,457 - +0.1 459,457 - +0.8 459,457 - +0.3 459,458 + -2.5 459,482 - +0.1 459,482 - -0.3 459,482 - +0.3 459,502 - -1.0 459,502 - -0.1
Or see this region's nucleotide sequence